U43 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101484

Myc-DDK-tagged ORF clone of viral ORF for helicase/primase complex [Human herpesvirus 6], codon optimized for human cell expression, NP_050224

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101484 represents NCBI reference of NP_050224 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTATAGTGGTTTTTGCGACCGAGTACGACGCTGCCAATGTGATCTTCTCCATTTTGTGCAGATCCC
  CATCCGAGCACTTGATTTTCCCAATTATCGTCAAGTACAAGCCTAGCAACAATGTGAGCTTCTGCCTGCA
  GACCCAGAAGTGCAAAAATTCCAAGCGGATTGATACAGTTTTCGTCTGCCACGCAGAGAAACTGAACCTC
  TCACACTACATCCAGACCGCATCCCCCATAAAGGCCGAGGACGTGGCCAATTCACTGAATGACAAGGAGA
  CAGAACTTCTGTATGTCGACATGATTTTGTCACAGACCGGGAAAGAGAAAGAAGATGTGGAGTTCAAGTA
  CATGGCCTACTTTCACAAAAGCCTGATCATTAAATATCTCACAGGTAAGTTCCTTCTGCCTACTTCTCCA
  TTTTGGTTCCTGAGCACTTATGGACAAACGGAAGGCCTGCTGTTGTTGACTATGTATTACTACCTGTTCG
  AAGAGCAAAAATCCACGATAACCACCACTAAAAATTACGTCCAGTGTTTTACAGAGAACACCGGCAGCAT
  GGTGTTCACCTACTCCTCAATGTCAGAATTTATTAATATAACCCTCAAGTCAAAGTTTCGCAAGCTGTTT
  GCGGACTTCGCCACTTATGCCAGGCAAAAGAATCTGAGGGATAAAGAAGAATTCAAGTACCTTGATACTC
  AGATTAACCTTTTCAGAAAGTCCTCCCATCTGACAAATACCTTTCGAGTGCACTACATTTACATAGCATA
  TAACACCGCCCTGGAGACTACAAAGTTCGTGAATTATTGCAACATCACGAGCTATGATTCTAACCTGCCC
  ATTGGCCAGCAGTGTCAGAGGAATGTGCATATTCTCGGAAATTCCCTGCATGAAAATCTGTTGTGTATCA
  TGAAGCAGTACTTTAACGCCGATTGCTATTTTAAAACCTATATTGATATTAAACGGCTCAAGAACCCGGA
  TCTTAACGTGACCGAGTATGAGTACGCACTGGCCTCCAAGAAAAAAACTATTCAGGCTCTCACATCAGAG
  CAAATCACGCGCGCAATCGCTAAATGCAACAAAAACGGCGAGGGTTTGTTTTCCCCAGTTAAACCAGGGC
  TCCAGGGCTTGCTTGAAATATCTGCCAGCGATAAATATGTCCAAATTCAGGACAAGAGAATTTACCGCAG
  ACAGCATCTCCACAAAGATTACCACCGCCCATTCCCAGTGTTCAGAGTCCAACTTCTTCACAAGAACATT
  TTTTGTTTTGGGAATTCCGAGGACTGGTATGAGAACATGGGGTTCAATAGGATCTTGCAGTACTTGCCGG
  ACGAGTACATAAGCGATGAAGCCCTGACGCGAGCGATATGGCTGCAGGACACTCATTTCCTGTGTGACGA
  CTTCGAGAAGCAGTTTTACACCACCAGACATGAGATCTTCAACGAGAGAATACCAGTCACAAACTATATT
  GGAGATCTCGACCTCCCCCTCCAGGACACAGCCACTATAACTGAGGAGACATTCTTTTCTATGTGTCGGC
  TTATCCGATTGACCTTGATTAACGCCTGGAAGAAAATTTTCCCCAGTATCGATACCCATACCCATCCCAT
  CTTCTTCTTCAAGACCCAGTGTGACACTACAAACGACGCACTGGATTACACTGAGGACCCCACTGAGATT
  AAGCAGTTCTGTATTTGCAGAAAGAAGATCGGGCTGCGAATTTCTATTCCTCTGCCCAACGGCACTGCCA
  TTGCCGGGGGCGAACCCCTGAAACAGCTCTCTAAAATTCTGAACCACGTTATGTGCTTGGACCAGGAGCT
  GAGCCAGATCCTTAATTCATTGACATTTCCTGGAGAATGCTTTGACATTGGAATCTACCATACCGGTCAC
  TGCATAAGAATCGGCTACATGTATAAAACCGACATGGATAAAGGCAAGATGCTGCACGGCCGGCTGACCC
  CTATCTTTATCGTGCCCGAGGGCTACAGAAATTCCTGCAAGACGTTCATTCAGATGCAGATGGACCTTAA
  CAATCTGTTGCATCATGGCACCAAGAAAGCTCCTATCGAAGAACTGATATATTCTATTACAGACAAGGGC
  TGTCCCAAAGAGAACCTGTCCTTCATGGATCTGAAGAGTAGACAGCTCTGGAATAAAGTGAACATTGCTA
  CAGATACTTTGATCACCAAGTACCTCAACACTCACGGATTTAACAATAATGCGACATCAGCTGATGATTC
  TCTCCTGAGCTTCATTCGCCTGATTGGATGGCCCATAATTAAAACTCAACTGATCACCCATTACGAGACC
  AGAATCGCCCAGCAATTCTCCCAGGTTACCTTCCTGAAGATCGACTCCAAAAACCTGCAGATTAAAAAGA
  CACAGTTTGGCCGAGTGAGCGATTTTAGTTGTCTGAATAGACAGCACAGGGGGAATAGAGACAATGTACT
  GGTGTATATACAGTTGAAAGCCGATGGAAACCGACTGATCCTCATCCTGTGGAGCACTTGCTTCGCTACT
  AAATGTCAGAGCAACTCCAAACAGGTCCACTGCAGTATCGCACTGGAACAACTGAAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101484 representing NP_050224
  Red=Cloning sites Green=Tags
  
  MTIVVFATEYDAANVIFSILCRSPSEHLIFPIIVKYKPSNNVSFCLQTQKCKNSKRIDTVFVCHAEKLNL
  SHYIQTASPIKAEDVANSLNDKETELLYVDMILSQTGKEKEDVEFKYMAYFHKSLIIKYLTGKFLLPTSP
  FWFLSTYGQTEGLLLLTMYYYLFEEQKSTITTTKNYVQCFTENTGSMVFTYSSMSEFINITLKSKFRKLF
  ADFATYARQKNLRDKEEFKYLDTQINLFRKSSHLTNTFRVHYIYIAYNTALETTKFVNYCNITSYDSNLP
  IGQQCQRNVHILGNSLHENLLCIMKQYFNADCYFKTYIDIKRLKNPDLNVTEYEYALASKKKTIQALTSE
  QITRAIAKCNKNGEGLFSPVKPGLQGLLEISASDKYVQIQDKRIYRRQHLHKDYHRPFPVFRVQLLHKNI
  FCFGNSEDWYENMGFNRILQYLPDEYISDEALTRAIWLQDTHFLCDDFEKQFYTTRHEIFNERIPVTNYI
  GDLDLPLQDTATITEETFFSMCRLIRLTLINAWKKIFPSIDTHTHPIFFFKTQCDTTNDALDYTEDPTEI
  KQFCICRKKIGLRISIPLPNGTAIAGGEPLKQLSKILNHVMCLDQELSQILNSLTFPGECFDIGIYHTGH
  CIRIGYMYKTDMDKGKMLHGRLTPIFIVPEGYRNSCKTFIQMQMDLNNLLHHGTKKAPIEELIYSITDKG
  CPKENLSFMDLKSRQLWNKVNIATDTLITKYLNTHGFNNNATSADDSLLSFIRLIGWPIIKTQLITHYET
  RIAQQFSQVTFLKIDSKNLQIKKTQFGRVSDFSCLNRQHRGNRDNVLVYIQLKADGNRLILILWSTCFAT
  KCQSNSKQVHCSIALEQLKN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050224
• RefSeq ORF: 2580
• Locus ID: 1497045
• MW: 100.0 kDa