U52 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101493

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp058 [Human herpesvirus 6], codon optimized for human cell expression, NP_050233

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101493 represents NCBI reference of NP_050233 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCAAATAGGACAGTATATAAAGCTCAATGATGCTGTTCCAAACCTCATCCTGCACATCACAACAA
  AACTCCTCAGGAACGAGAATTTGACGAGTTTCAAGCAGGAGGAACTCCTTCTGATTCAGCATGTGTGCAC
  GTCCATGCTGTCACACGGGATCAAGATTCTTCTCCTTAGGGAATCACTCTACAATAGTGGGATTGGCGAT
  ATAGTAATCCTGAATAGGAAGATCAGCAATAATTATTGGTTTAGGCTGTTCTCCATCCTTAAACAACACA
  GCGACGCTGAACTTCTTAGACACATGTTCAATGAGTCACATAGTGCCTACATCTCAAAAAAACTGCATTA
  CTCTGGCAACGTTTCACACATCATAAATTTCCTCTTTATGGATGAGTTCGGCTTGAGCTTGAAAATTCCA
  GAAGAGATCATTTGTGAGGGCAACATTGTCTTTTCCGTGGGCGCCATCTATAATCATCGGCTCCTTAAGA
  TATGTAGATTCTTTAACCGCTTCTGGGGCGACCAGGAGAGGGAACCTGCCGTGCGACTGATCTGTAAGCA
  CCTGTGGTTTGCCTACTTGATTATGTTTGGGAAATTCGAAATCTCAACGCTTGCGTACAATCAGCAGAGA
  GCTGAGCATAAGGCAGGCCTGTTTAGCTTCCTCCAAAACGACTTCAAGGTCTTCTGCGGCATGTCTGAGA
  ATCCCCAGCTGTTGGACTCTAGCGCAATATTTGACCTCACAGGCATCTCAGCCGAGGATCTGTTTTCCTA
  TGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101493 representing NP_050233
  Red=Cloning sites Green=Tags
  
  MTQIGQYIKLNDAVPNLILHITTKLLRNENLTSFKQEELLLIQHVCTSMLSHGIKILLLRESLYNSGIGD
  IVILNRKISNNYWFRLFSILKQHSDAELLRHMFNESHSAYISKKLHYSGNVSHIINFLFMDEFGLSLKIP
  EEIICEGNIVFSVGAIYNHRLLKICRFFNRFWGDQEREPAVRLICKHLWFAYLIMFGKFEISTLAYNQQR
  AEHKAGLFSFLQNDFKVFCGMSENPQLLDSSAIFDLTGISAEDLFSYE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050233
• RefSeq ORF: 774
• Locus ID: 1497054
• MW: 30.0 kDa