U49 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101490

Myc-DDK-tagged ORF clone of viral ORF for putative fusion protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050230

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101490 represents NCBI reference of NP_050230 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTCACCGGTTTGCCTGATATACGGAAGAAGATTGGACAGTTCCACCATCTCAGGATCTATAAGC
  AAATTTTGAGTCTTCAAGGTAACTTCGCTAGACTGAATTATTTCCTGGGCGACGTTTTTCCAGCCAACCT
  CAGATCTGCATCTGTCTCTGTGTTTTTCGAGGTGAGACTGGGGCCACGGATTCCCGACTGTATCGTTCTG
  CTCAAGTCTGTGGACGCTAAGGACGAATTCGCTTTCCACTGCTACTTCTTTGAATTTAAGACCACTCTCG
  GCAAGAGTACAATGCAAAGTGTGCATCACAACTGCATCCACCAGGCTCAGTACCTGCAGGGACTCCGGCA
  GTTGCACCAATCTATCTCTTTCCTGGACCAATATCTTATTGCCGACGAAGTCCTGTGGAACGTGGTCCCC
  GTTATTTGTTTTTTCAGACAATGGGGACTTAAGCTCGACTTTTTCAAGAAATTTTCCGGAAAGACAAAGC
  GCCTCTCATTCTCTTTCATATGCGATCTCTTCGCACGCAGTCAGGACGGCGCTGTGCAATCTCTCCTTAG
  CATCCCCAATTATACCAACTTTCGGCGGGCCTGTCAGAAACATACGGACTTGTATAGACGGCGCTATCAG
  AAGGCAAGTAAGTCAGTGCTGACAAAGACCAGCGGTGAGAATCGAAGCCGCGCGAGCCGCCAGGTAGCGA
  AGAATGCCCCAAAAAACAGGATCCGGCGCACAGCCAAGAAGGACGCAAAGCGGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101490 representing NP_050230
  Red=Cloning sites Green=Tags
  
  MSLTGLPDIRKKIGQFHHLRIYKQILSLQGNFARLNYFLGDVFPANLRSASVSVFFEVRLGPRIPDCIVL
  LKSVDAKDEFAFHCYFFEFKTTLGKSTMQSVHHNCIHQAQYLQGLRQLHQSISFLDQYLIADEVLWNVVP
  VICFFRQWGLKLDFFKKFSGKTKRLSFSFICDLFARSQDGAVQSLLSIPNYTNFRRACQKHTDLYRRRYQ
  KASKSVLTKTSGENRSRASRQVAKNAPKNRIRRTAKKDAKRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 756 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050230
• RefSeq ORF: 756
• Locus ID: 1497051
• MW: 29.3 kDa