U63 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101502

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp068 [Human herpesvirus 6], codon optimized for human cell expression, NP_050243

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U63
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101502 represents NCBI reference of NP_050243 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGGCACTTCCACGCAAATACGATAGAGTCACAGGTCGCATCCTGACACATAAAAATAACCAGA
  TGTGCACTACAGAGTGTAGCCAGATGTATAATCTGCATAATCCCATAACTTTTGAACTGGGCCTCGGGAA
  CGTTTTCGTGTGTATGCGATGCTTGACCGTACACCATTGCGACATGCAGACTGATTGTACTATTGTCAAC
  ACCCATGAAGGATACGTATGCGCCAAAACAGGCCTGTTCTACAGCGGATGGATGCCCACTTATGCGGATT
  GCTTCCTCGAGCCCATTTGCGAGCCTAACATAGAGACAGTCAATGTTGTCGTTGTCCTCCTGAGCTACGT
  GTACAGCTTTCTTATGGAAAACAAAGAACGGTATGCCGCTATCATCGACAGTATCATAAAGGACGGCAAG
  TTTATCAAGAATGTGGAGGATGCCGTGTTTTACACCTTCAATGCCGTTTTTACAAACTCTACATTTAACA
  AGATACCGCTTACCACAATCAGTCGGCTCTTTGTACAGCTGATCATCGGTGGCCACGCCAAAGGGACAAT
  ATACGACAGCAACGTCATCCGCGTGAGCAGACGCAAGAGAGAGGACAGCTTGCTGAAGAAGATGCGCCTG
  GAGTACGGCAACGCCCTTATTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101502 representing NP_050243
  Red=Cloning sites Green=Tags
  
  MEMALPRKYDRVTGRILTHKNNQMCTTECSQMYNLHNPITFELGLGNVFVCMRCLTVHHCDMQTDCTIVN
  THEGYVCAKTGLFYSGWMPTYADCFLEPICEPNIETVNVVVVLLSYVYSFLMENKERYAAIIDSIIKDGK
  FIKNVEDAVFYTFNAVFTNSTFNKIPLTTISRLFVQLIIGGHAKGTIYDSNVIRVSRRKREDSLLKKMRL
  EYGNALIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050243
• RefSeq ORF: 654
• Locus ID: 1497063
• MW: 25.0 kDa