U22 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101463

Myc-DDK-tagged ORF clone of viral ORF for glycoprotein [Human herpesvirus 6], codon optimized for human cell expression, NP_050202

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101463 represents NCBI reference of NP_050202 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCCCCAGGGGTGGAGTCTGGCCTGGGTCAGTGTTTTGTACGTGAGTGTCATTCCATCTTTGCACA
  TCATCAACAACGAGAACTCCGTATTTATCGGCACACACTCAGAAACAGAGCTTCGGCACTGGCTTATTTT
  CGTCAAAATGGCCCAGAGATCCGGGACAGCCTGGTGGCGGATGGCCTCCGTGCCGATCAATGCATACTTC
  GAAAGAGACATTGCATTCCTGTTTAATCCAAGATGTGTGATCGAGACTGCTCTGGGCTCCAAGATCCTGT
  GCCGGTATAATAAAAATATCGGCGTTGTGTTTGTAGATAACGACACCACCTGTAATGTCTCCTTCCCTAG
  TGGGGTCCAGCTCCAACTCCTGAATCAGTCCGTAATGGAGAGCATACGGACCAAGACTTACGTTGTCGAC
  TATGCTAGAAAAACCACCGAACGCGGAGATTGCTTTATTTCTGTCGCCTTCTGCAGGAAGGAGAGGAGGC
  GCTTTCTCCCCCGGTACGAGAGGTTTGTGTACTATTGCATCTCCGTTTATCTCTTTGCCGTCGCTGTGTT
  CTGCTCTTGTTGGTTTGCCCTCGACCCACTCTTTAATATGTGGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101463 representing NP_050202
  Red=Cloning sites Green=Tags
  
  MVPQGWSLAWVSVLYVSVIPSLHIINNENSVFIGTHSETELRHWLIFVKMAQRSGTAWWRMASVPINAYF
  ERDIAFLFNPRCVIETALGSKILCRYNKNIGVVFVDNDTTCNVSFPSGVQLQLLNQSVMESIRTKTYVVD
  YARKTTERGDCFISVAFCRKERRRFLPRYERFVYYCISVYLFAVAVFCSCWFALDPLFNMWA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 606 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050202
• RefSeq ORF: 606
• Locus ID: 1497024
• MW: 23.5 kDa