U46 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101487

Myc-DDK-tagged ORF clone of viral ORF for putative membrane /secreted protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050227

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101487 represents NCBI reference of NP_050227 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTGCAAAAAAGGCGCTCGGCAGCGGCTGTATGTGTCCTTGTGGCTCTTTTATATTCTGGTGTTCG
  CCGCAGCCACCGAGATGGATTTCTACTCATCCGAATGTCACTCTCACACTTATGAAATAGTCTTGAACAG
  CTTTTCCAGTATCTGGCTCCTTATAAACTTGTTTCTGCTCCTGTGCTCATTTGCTATCTTCCTGAAGTAT
  TGGTGTTACAAGACTTTCGCCAGTGAAACCGTCAAAGGATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101487 representing NP_050227
  Red=Cloning sites Green=Tags
  
  MSCKKGARQRLYVSLWLFYILVFAAATEMDFYSSECHSHTYEIVLNSFSSIWLLINLFLLLCSFAIFLKY
  WCYKTFASETVKGY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 252 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050227
• RefSeq ORF: 252
• Locus ID: 1497048
• MW: 9.9 kDa