U67 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101506

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp071 [Human herpesvirus 6], codon optimized for human cell expression, NP_050246

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101506 represents NCBI reference of NP_050246 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGTAATGTCCCAGGTCAGGTCCATGGAACCAGACCTGACCCTGGCAGCTGTTTATCAGGCAGCTG
  CAAATCTTACAGAACAAGATAAGGAGATCTTCTCTGAAGCCGTGAAAACTGCATTCAGTGTGTGCAGTAG
  CGCCGCCCCCTCTGCGCGGCTCCGGATGATAGAAACACCTACGCAGAACTTTATGTTCGTCACCAGCGTT
  ATCCCAAGCGGGGTCCCTTCTGGGGAGAAGAAGACAAAACTCAACATTGACGCTGCACTGGACAATCTCG
  CTCTTTCTTTCGCTAATAAGAAGTCCAAAAAAATGGCCCGGACTTACCTGCTGCAGAATGTTTCCCGGAC
  CCAGGATCAGCAGGTGGCCATAAGCGGAACTTATATCCTCTACACCAAGAAGCACATTGAAACCTCACTG
  ATGCTGGATAAGACAAAGCTTGTCAAGCAGATACTGGAGTACGCCGAGACCCCCAATCTGCTGGGGTACA
  CCGATGTTCGAGACTTGGAGTGTCTGCTGTGGCTGGTTTTTTGCGGTCCTAAGAGCTTTTGCCAGTCAGA
  TAGTTGTTTCGGATACTCCAAGACCGGGTACAACGCGGCTTTCCCCAACCTGTTGCCACCCTATCTGTAT
  GAGTGTGGCCAGAACAACGGTCTGTTCTTCGGAATCGTCCAAGCGTACGTATTCAGCTGGTATAGCGATT
  TTGACTTCTCTGCACTTGAGATATCAGAGCGAGCGCGGCGCAGGATTCGGAGTCTTCTCTACGATTTGAA
  GCAGAAATTCTCTGAGCAGGAGATTTCCGTTCTGCCAGTGGCCAGCCAGATGTGCATCTTCTGCGCACTG
  TATAAACAGAACAAGCTCTCCCTGGAGTACGTGTCTGGAGATCTCAAGACAAGCGTTTTTTCTCCCATCA
  TTATCAAGGACTGCTTGTGCGTGCAGACAACTATCTCAACGACCCAGATGCTGCCAGGTACTAAATCCTC
  CGCCATTTTTCCTGTATACGATCTCCGCAAGCTGCTCTCAGCCTTGGTGATCAGCGAAGGGAGCGTAAGG
  TTTGACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101506 representing NP_050246
  Red=Cloning sites Green=Tags
  
  MWVMSQVRSMEPDLTLAAVYQAAANLTEQDKEIFSEAVKTAFSVCSSAAPSARLRMIETPTQNFMFVTSV
  IPSGVPSGEKKTKLNIDAALDNLALSFANKKSKKMARTYLLQNVSRTQDQQVAISGTYILYTKKHIETSL
  MLDKTKLVKQILEYAETPNLLGYTDVRDLECLLWLVFCGPKSFCQSDSCFGYSKTGYNAAFPNLLPPYLY
  ECGQNNGLFFGIVQAYVFSWYSDFDFSALEISERARRRIRSLLYDLKQKFSEQEISVLPVASQMCIFCAL
  YKQNKLSLEYVSGDLKTSVFSPIIIKDCLCVQTTISTTQMLPGTKSSAIFPVYDLRKLLSALVISEGSVR
  FDI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050246
• RefSeq ORF: 1059
• Locus ID: 1497067
• MW: 39.6 kDa