U65 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101504

Myc-DDK-tagged ORF clone of viral ORF for tegument protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050245

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101504 represents NCBI reference of NP_050245 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATCAGTACATTTTCCATTGGTGACCTTGGATACTTGCGCAATTTCCTCCAAAATGAGTGCAATT
  GGTTCCGCATCTGTAAGAAAACGTTTTATAGAGAGTACAGAAGCGTCGCGACCTCAAGTCCAATCTTTAG
  TTTGAAAAATAAGCCTAAGAAATACTGTATGCATTGTGAGATGGTGGTACTTAAGAGAAGCCATGAGTTC
  ATGTTCTCTCTGGCGGTAAATGGGATCCACTTTGGCCAGTTCCTCACCGGGATTATGAAATTTAAGAAAA
  AGCAGGTGGCTGAGGGGCTCTGCTATTATGTGTTGGAGTTGGGATCTATTAGTCCCGTAGACCTGAGCTT
  TATCCCAAAGTACAACAGTGATTGCGTGACTTCAATGCATTGCGTGACTCCAGAGTTGATTTATGAAAAC
  TGCAGTATCGTGTGTCCCGAGGAGGCAAGCCGGCTTACCGTGAAGGGCCTCGGAGACAATAAGTTGATTC
  CACTGGGGGGATGCGGAGTGTGGTGCCTCAAAAATGGTGGAGACCTCTACATCTATGCTTTCGTCCTGGC
  TTACGATCTGTATGTCGCTTGCTATGATAAGACAATATTCCCAAGCTTGGCCAAAATCGTTTTCGACATG
  ATAGCCTGTGACTCTGAGGATTGTGTATTCTGTAAAGATCATAACAAGCATGTGTCCCAGGCGGGCCACA
  TCGTCGGGTGTGTCTCAAATCAGGAGACATGCTTTTGCTACACGCCCTGCCAAAAAAAAATGACCGACAT
  CAATAACCCCGAACTGATCTCTCTCCTGTGCGACCAAGAAATTAACAAAATCGACATTATGTACCCAGAG
  AAGAAGGCCTCCCTGTCACTCGATATCAATTCATACGTGCACGGATATCTGGGCGATGAACCCTGTGCTC
  TCAAATGTGTCAATTGGATGCCAATTCGGATCTCATCCGCTCTCAGCCGACTTATCATTTTGTCATGCCC
  TGTTTGTAAACGGGTCGTGATGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101504 representing NP_050245
  Red=Cloning sites Green=Tags
  
  MAISTFSIGDLGYLRNFLQNECNWFRICKKTFYREYRSVATSSPIFSLKNKPKKYCMHCEMVVLKRSHEF
  MFSLAVNGIHFGQFLTGIMKFKKKQVAEGLCYYVLELGSISPVDLSFIPKYNSDCVTSMHCVTPELIYEN
  CSIVCPEEASRLTVKGLGDNKLIPLGGCGVWCLKNGGDLYIYAFVLAYDLYVACYDKTIFPSLAKIVFDM
  IACDSEDCVFCKDHNKHVSQAGHIVGCVSNQETCFCYTPCQKKMTDINNPELISLLCDQEINKIDIMYPE
  KKASLSLDINSYVHGYLGDEPCALKCVNWMPIRISSALSRLIILSCPVCKRVVMD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050245
• RefSeq ORF: 1005
• Locus ID: 1497065
• MW: 37.9 kDa