B1 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101443

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp003 [Human herpesvirus 6], codon optimized for human cell expression, NP_050178

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101443 represents NCBI reference of NP_050178 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAAAATATGAAGACCAAGAAAACCAAAAAAAGAGGGAGAAAAGAGGGTAATACCCCCGAAACCG
  AAAGGCGAATGGAACCAGCCCGGTCCAGAACTTCAGCTATCCCCTCAGGACTTAGACGCAGAAGCGGCCC
  CAGTACCCCCCTGAGACCTGGACCAGAAGTGCGCCATGCCCCCACATGGAGAACCGCGTCTGCCACGACC
  GCCGATTCCCATAGGATTTCTCCACCTTACACCCCCAGCAGTAGAGGTCGCCACATCCACACAAGGGGCG
  CCCGCACACGAACCCGGGAGACCTCAGCAGCCGAAATTAACGGGGTCTACGCAAGGGCCGTGACCCGAAA
  GACTAAACGATCCGAGACCATCGACAGGCTGCTTCTGTCAGTCCTGCCCGGCCACGGACCTCATGCATCT
  CTGCGGAGCCACTTGCGAGCACGGAGCGCTTTGCGACCGCCACCCGATCCTCCGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101443 representing NP_050178
  Red=Cloning sites Green=Tags
  
  MQKNMKTKKTKKRGRKEGNTPETERRMEPARSRTSAIPSGLRRRSGPSTPLRPGPEVRHAPTWRTASATT
  ADSHRISPPYTPSSRGRHIHTRGARTRTRETSAAEINGVYARAVTRKTKRSETIDRLLLSVLPGHGPHAS
  LRSHLRARSALRPPPDPPR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 477 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050178
• RefSeq ORF: 477
• Locus ID: 1497000
• MW: 17.8 kDa