U45 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101486

Myc-DDK-tagged ORF clone of viral ORF for putative dUTPase [Human herpesvirus 6], codon optimized for human cell expression, NP_050226

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101486 represents NCBI reference of NP_050226 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCTGTGATCTCCGAAAAAATCAGTGAAACAATCACCCTGCAGAGACAGACTTCTAGTAGATACA
  TTGAATTCTTCGTGTTCAGGAATGTGGACATCAATGAGCTTTGGACTACGGACATTTCCGAGGATAAGAC
  CCACGACGTTTGGCCCGCAATTAATGAAAAATCTTTCAAAAAATTCCTGGAGAATGAACTGACCAGTTAT
  CAGAGGCCCATCTCCCTCCTTGGTATACCCCAGAATGGCACCGTATCTAAAACCTGCAAGAGGGAGAAAC
  AGAGAGAGACGGATTGCGTAAATTATGTGAGGAAACATGGCAACCCCGTGACGTTCTATCCAAGACACAG
  AGCGAAGAGGAACGCCAACACGGACACGTGCATCAGTGAGGAGCCGTCCATCCTTGTCTCACACCACAGG
  AACAGCAAGATGGATGTGTTCATGGATGCAAACAAAATTACCCTTGTCAATCGGGAGCTGATTTGGGTCC
  CTCACGATCAAGTCCGGATCGTGAAGCTGGACATCAGTCTCTGTATCCCCGATGGGTTTTTTGGGGTCAT
  CATCGGCCACAGCAACGACGTGTTTTGTGAATGTATCACTGAAATCATAACTGATGAGACGGACATCAGC
  GTCTTCCTCATGAATCTCTCCGAACACTCTCTGATGCTGCTGCCTGGGGATGTAGAGTTTTCAATTAACT
  TTCTCCCATGCTATATTCCGGAGCCATGGGAGATGATAAACCTGAGCCCCCCTGAGAGTGCAGTGTTTCA
  TCTGAAGACCTGCCGAGAGTTTATCATCAAGCCTAATTCCTACACCATTCAGTGCTTTGACGCTATGTAC
  GTGTGTGCAGACGAGCTTAAGGCACTGATGATTCCCAGTAAAGAGATAATAAAACTGGGTCTCCTTATTG
  AAACTTATATATGGAATAAGGACACCATCCCATCCATAAAAATATTTAACTCCACACGGAAAACCATTTA
  CATACCGACTGGCATCTGTATAGCTCGAATCATATTTACCTGCGGGCACTTTTGCCTGTCCCTCATGCCA
  GAACGGGCAATTAACCGATTGCAGGTGTTGGACGCAAGCTCATTTTTCCTGTTTCACTATGCAGCATTCT
  CTAACGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101486 representing NP_050226
  Red=Cloning sites Green=Tags
  
  MYSVISEKISETITLQRQTSSRYIEFFVFRNVDINELWTTDISEDKTHDVWPAINEKSFKKFLENELTSY
  QRPISLLGIPQNGTVSKTCKREKQRETDCVNYVRKHGNPVTFYPRHRAKRNANTDTCISEEPSILVSHHR
  NSKMDVFMDANKITLVNRELIWVPHDQVRIVKLDISLCIPDGFFGVIIGHSNDVFCECITEIITDETDIS
  VFLMNLSEHSLMLLPGDVEFSINFLPCYIPEPWEMINLSPPESAVFHLKTCREFIIKPNSYTIQCFDAMY
  VCADELKALMIPSKEIIKLGLLIETYIWNKDTIPSIKIFNSTRKTIYIPTGICIARIIFTCGHFCLSLMP
  ERAINRLQVLDASSFFLFHYAAFSNA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050226
• RefSeq ORF: 1128
• Locus ID: 1497047
• MW: 43.4 kDa