U62 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101501

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp067 [Human herpesvirus 6], codon optimized for human cell expression, NP_050242

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U62
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101501 represents NCBI reference of NP_050242 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCGCCCTCAATGAGATTAAGGATGACTTTGAGAACTGTGAAACTAAAAATGATCTTTTCAAAA
  TTATAGATAAAATAAGTAAAAATTGCAACTTCATTGTCGAACAGGTCGAAAGTCTGCCCCGGAGGGTAGA
  TTCAGCCGCTATTCTCTTTGACAATTTGGCAGTAGAAATCTTTAACGACGTCATCTACAGACAGAACGGA
  GACGGGGTTGCAGCGAAGATCCGGCAGGGTAACGGACAGAATATCGATACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101501 representing NP_050242
  Red=Cloning sites Green=Tags
  
  MNSALNEIKDDFENCETKNDLFKIIDKISKNCNFIVEQVESLPRRVDSAAILFDNLAVEIFNDVIYRQNG
  DGVAAKIRQGNGQNIDT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 261 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050242
• RefSeq ORF: 261
• Locus ID: 1497062
• MW: 9.8 kDa