DR6 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101537

Myc-DDK-tagged ORF clone of viral ORF for transactivator [Human herpesvirus 6], codon optimized for human cell expression, NP_050277

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DR6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101537 represents NCBI reference of NP_050277 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACCCGGCACACACAAACGAGAGATGGGCGCATTGCAATTAGACGAGACGGTGCGAGGCTGGCAC
  ATGCAAGGGCCAGGGCCAGATTCGAGTGGCTCTTGTTGGCCAGGGGCAGGCCCAGTAAGTTGTACGGGTA
  CACCTCAAGGCATAGAGGGGAACGGATACATCTGCCTTGGCCCCGATACTGGTGTCTCGAACTGCATCCT
  GACCCCTACAGGGACGCAAGATCAGCCACTGTCTGGGGCCACCGATGGGGTTGGCCACCTACACATGTGC
  GACCCAGATCAGTGCAGGACTGTGCGCTGGACTCCTCCCTCTATGTGTGTTGCGGCTATGGGGAGAAACT
  GCAGCCAGTAGGATTTGTATCAAGTTACCTCACACACAGTCCGCTCGACACTCTGCGAGTGCTGCTGGTT
  GGTCGAGACGGCGCCGTGTATGTGCACCATATGAGGGCCGCTCGCTTGTGTCGACTTGCGTCTAACGTGA
  CAGAATTCGCCCGCCGGGGATTGCAACGGGATCCCGTTGCCTACGAAGAAGACCTGGAGCTGCCCGATAG
  GCGAATGTGTGGCACCAACGTGCGGCACCTTTTCGATGTGATTGCAGCAGCTGCTGACGAGCATGACCTG
  CTGACAGTTGGAGGGCTTTGTCAGACACACGCTGGGGTCTCCTGTGAGCTGCTGGAGACCGTGAGGGACC
  CTTGGACCGCTGTCCCAGGGGTGCGAATGACATTGACTGTGGCTAGAGCCCAGTATCGGTTGTGGCCCGA
  TGCAAGAAGGCAGCTGCGGCTCCACCTGTATGCTGGCCACCCCCTGGGACCGTGGATCGTCTGTGCCGTT
  CTGAGTCGGGAGCGAGAGACTCAGACACCGAGCCCTCCGATCGGGAGTGGCGGTGTTATACTGGGAAATG
  TGCCTACCCCAGGCCCGAGGGAAGTGGAGACTGCCTGGGTGATCGTGACAGTAGCTGGCCCACTTTTGTC
  ATTTTGGCCCGATAACGGAAAAATCTGCCGCCTCGCAAATTCATTTGCAGCCCTTTGGAGAATGGGACCA
  CGCGCCATGAGGGGCCATTGGACATACAGCGCTCCGGGCCGACATTTGCCTGGCGACGCTTGGCCGCTTT
  GCGAGCACGTTAGACCGCCTGTGGGGAAACTGCCCCGGCAACGCGCCTACCTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101537 representing NP_050277
  Red=Cloning sites Green=Tags
  
  MTTRHTQTRDGRIAIRRDGARLAHARARARFEWLLLARGRPSKLYGYTSRHRGERIHLPWPRYWCLELHP
  DPYRDARSATVWGHRWGWPPTHVRPRSVQDCALDSSLYVCCGYGEKLQPVGFVSSYLTHSPLDTLRVLLV
  GRDGAVYVHHMRAARLCRLASNVTEFARRGLQRDPVAYEEDLELPDRRMCGTNVRHLFDVIAAAADEHDL
  LTVGGLCQTHAGVSCELLETVRDPWTAVPGVRMTLTVARAQYRLWPDARRQLRLHLYAGHPLGPWIVCAV
  LSRERETQTPSPPIGSGGVILGNVPTPGPREVETAWVIVTVAGPLLSFWPDNGKICRLANSFAALWRMGP
  RAMRGHWTYSAPGRHLPGDAWPLCEHVRPPVGKLPRQRAYLD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050277
• RefSeq ORF: 1176
• Locus ID: 1497098
• MW: 44.3 kDa