U47 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101488

Myc-DDK-tagged ORF clone of viral ORF for glycoprotein O [Human herpesvirus 6], codon optimized for human cell expression, NP_050228

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101488 represents NCBI reference of NP_050228 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCATATCTCAAGACTGGGGCTCTTCCTTGCCCTGTTCGCTATTGTGATGCATTCCGTGAACCTGA
  TTAAATATACTTCAGACCCTCTGGAAGCCTTCAAGACCGTGAACCGCCACAATTGGTCTGATGAGCAGCG
  CGAGCACTTTTACGACTTGAGAAATCTCTATACTACTTTCTGCCAGCGGAACCTCAGCCTTGATTGCTTT
  ACACAGATTCTGACCAATGTCTTTTCCTGGAACATCCGGGATCTTCAATGCAAGTCAGCGGTGAATCTGA
  GCCCACTCCAAAACCTCCCGAGGGCCGAGACCAAAATTGTCCTTAGCAGTACTGCTGCAAATAAAAGCAT
  CGTGGCCTCAAGTTTCTCCCTGTTCTACCTGCTCTTTGCTACTCTTTCAACATATACCGCTGACCCCCCA
  TGCGTAGAGCTGCTGCCATTCAAGATTCTGGGAACCCAGTTGTTCGACATTAAACTGACCGATGAGTCTC
  TTCAGATGGCTATTTCTAAGTTTAGCAATAGCAACCTCACGAGGAGTCTGACACCATTCACTCCCGAGAT
  CTTCTTTAACTACACCAGTTTCGTCTATTTCCTCCTGTACAATACAACAAGCTGTATTCGCTCAAACGAC
  CAGTACTTTGAGCATTCACCGAAACCTATAAATGTGACCACCTCATTTGGACGAGCTATAGTTAACTTTC
  ATTCCATTCTCACGACCACTCCGTCCAGCACTCCTAGCAGCACATCAGCCTCCATAACATCACCTCATAT
  TCCATCCACCAACACCCCAACCCCGGAGCCGTCACCTGTGACCAAGAATTTTACTGAGCTTCAGACAGAC
  ACGATCAAAGTAACCCCTAACACCCCCACTATCACCGCGCAAACGACAGAGTCAATCAAGAAGGTCGTTA
  AGCGCAGCGACTTTCCTCGGCCAATGTATACTCCCACGGACATTCCAACTCTGACCATCAGACGGAACGC
  AACTATCAAAACCGAGCAGAACACAGAGAACCCTACTGAAAATCCAAAATCTCCCCCAAAACCTACGAAC
  TTCGAGAACACTACTATTCGGATCCCCGAAACCTTTGAGAGCACCACTGTTGCCACCAATACTACACAGA
  AGCTTGAAAGTACGACATTCGCAACTACCATCGGAATAGAAGAAATATCTGATAACATTTATTCATCACC
  CAAGAACAGCATATATCTGAAAAGTAAGTCACAACAGAGTACCACAAAGTTCACAGACACTGAGCACACG
  ACGCCCATCTTGAAGTTCACGACCTGGCAAGATGCTGCCCGCACATATATGAGCCACAATACCGAAGTGC
  AGAACATGACTGAGAATTTCATAAAGATTTCCCTGGGCGAGACTATGGGGATCACACCAAAAGAACCCAC
  TAATCCGACACAGCTCCTTAATGTTAAGAATCAGACAGAATATGCCAACGAAACTCACAGTACAGAAGTA
  CAGACTGTGAAAACTTTTAAGGAGGATCGGTTCCAGAGGACTACATTGAAGTCTAGCTCCGAGCCCCCAA
  CAGTACAGACCCTTAGCGTTACCCCAAAGAAGAAGCTGCCAAGCAATGTCACTGCTAAAACCGAGGTGCA
  GGTGACTAATAATGCACTGCCATCATCTAACAGTTCTCACTCAATAACTAAAGTTACTGAGGAACCTAAG
  CAAAACCGCATGTCTGCGAGTACTCATGGAGAGATTAATCACACCGAGATCCCACGCATGACCCCAATCC
  TGAATGCCCACACCTGGGAAAAGTCCACAACTCCGCAATGGCCCTTCACTGCAGAGACAAGTCTCACGAC
  CTCTAGCAAGTCTGCTATCCTGACGTGGAGCAACCTTCTCACTACACCCAAGGAGCCGCTGACAAATACT
  TCTCTCAGAAGCACTAACCACATTACGACCCAGCTGACGACCTCTAACCGCACCCAATCCGCTAAGCTGA
  CTAAGGCGCATGTCTCTTCCCAAACCACAAACATTTATCCACAAACAATAACTGAAAGAAGTACCGACGT
  GAAAAAAAAGAGTTCCACGGAGTCAAGAGAGGCTAATAAGACTCTGCCTGGCAACGACTATCGCGTAACA
  GATAAAAACAGCCATAACCATCCGGACAACCTGACTACCAAGGCTTACAGCACACAAAATGCGACCCACT
  ATACCTATAATGAGAGACACGACCTCAACAACACAGATAGCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101488 representing NP_050228
  Red=Cloning sites Green=Tags
  
  MLHISRLGLFLALFAIVMHSVNLIKYTSDPLEAFKTVNRHNWSDEQREHFYDLRNLYTTFCQRNLSLDCF
  TQILTNVFSWNIRDLQCKSAVNLSPLQNLPRAETKIVLSSTAANKSIVASSFSLFYLLFATLSTYTADPP
  CVELLPFKILGTQLFDIKLTDESLQMAISKFSNSNLTRSLTPFTPEIFFNYTSFVYFLLYNTTSCIRSND
  QYFEHSPKPINVTTSFGRAIVNFHSILTTTPSSTPSSTSASITSPHIPSTNTPTPEPSPVTKNFTELQTD
  TIKVTPNTPTITAQTTESIKKVVKRSDFPRPMYTPTDIPTLTIRRNATIKTEQNTENPTENPKSPPKPTN
  FENTTIRIPETFESTTVATNTTQKLESTTFATTIGIEEISDNIYSSPKNSIYLKSKSQQSTTKFTDTEHT
  TPILKFTTWQDAARTYMSHNTEVQNMTENFIKISLGETMGITPKEPTNPTQLLNVKNQTEYANETHSTEV
  QTVKTFKEDRFQRTTLKSSSEPPTVQTLSVTPKKKLPSNVTAKTEVQVTNNALPSSNSSHSITKVTEEPK
  QNRMSASTHGEINHTEIPRMTPILNAHTWEKSTTPQWPFTAETSLTTSSKSAILTWSNLLTTPKEPLTNT
  SLRSTNHITTQLTTSNRTQSAKLTKAHVSSQTTNIYPQTITERSTDVKKKSSTESREANKTLPGNDYRVT
  DKNSHNHPDNLTTKAYSTQNATHYTYNERHDLNNTDST
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050228
• RefSeq ORF: 2214
• Locus ID: 1497049
• MW: 83.0 kDa