U48 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101489

Myc-DDK-tagged ORF clone of viral ORF for glycoprotein H [Human herpesvirus 6], codon optimized for human cell expression, NP_050229

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101489 represents NCBI reference of NP_050229 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTTCAGGCTGTGGGTGTTTGTCCTGCTGACCCCCTGCTATTCATGGAGACCATGGACTATCAGCG
  ACGAGAGCCACTGTAAAAATGGTAATTCCGAAAATCCTATCGTCCGACCAGGCTTCATAACCTTCAATTT
  TTACACCAAGAACGACACACGCATCTACCAGGTGCCCAAGTGCCTGTTGGGAAGCGACATTACATACCAC
  CTGTTTGACGCCATAAACACTACAGAATCCCTCACCAACTACGAAAAGAGGGTGACCCGGTTTTACGAGC
  CTCCTATGAATGATATACTCAGACTGTCAACCGTTCCTGCTGTGAAGCAATTCAACCTGGACCACTCTAT
  TCAACCCCAAATCGTATACAGTCTGAATCTCTATCCCAGCCACGGGATCTACTATATACGGGTGGTCGAG
  GTGCGGCAAATGCAGTATGATAATGTGTCCTGCAAACTCCCTAACAGCCTCAACGAGCTTATATTTCCAG
  TCCAGGTGAGGTGCGCCAAGATAACCAGGTACGCCGGCGAGAACATATACACTCACTTTTTCACACCAGA
  CTTCATGATTCTTTACATCCAGAACCCGGCTGGAGATCTTACTATGATGTACGGAAACACAACCGATATT
  AACTTCAAAGCTCCATATCGAAAAAGCTCCTTTATATTTAAACAGACGCTTACCGACGATCTGCTGCTTA
  TTGTTGAGAAGGACGTGGTCGACGAGGAGTATAGGTTCATTTCTGACGCCACCTTCGTGGATGAAACCTT
  GGACGATGTGGACGAGGTCGAGGCTCTGCTGCTCAAGTTTAACAATCTGGGCATTCAGACTCTGTTGCGG
  GGGGATTGTAAAAAGCCAGATTACGCTGGCATCCCACAGATGATGTTTCTGTACGGTATTGTCCACTTCT
  CATATTCCACTAAGAATACTGGGCCAATGCCTGTATTGCGGGTCTTGAAAACACACGAAAACCTGCTGTC
  AATTGACTCCTTTGTTAATCGGTGCGTAAACGTATCCGAAGGCACGATACAGTATCCCAAAATGAAAGAA
  TTTCTGAAGTACGAGCCTTCCGACTACTCATATATTACCAAGAATAAATCAATCCCGGTGAGCACCTTGC
  TGACGTATCTGGCTACTGCCTACGAGACGAATGTAACAATCAGTCGGTACAAATGGAGTGACATCGCCAA
  CACACTGCAGAAGATATACGAAAAGCACATGTTTTTTACGAATCTGACATTCTCTGATAGGGAAACCCTG
  TTTATGCTGGCAGAGATTGCGAACTTTATTCCTGCGGACGAACGAATGCAGCGACACATGCAGCTGCTCA
  TTGGCAACCTGTGTAACCCTGTGGAAATCGTTTCCTGGGCTCACATGCTGACGGCCGATAAGGCACCTAA
  TTTGGAAAACATCTATAGTCCCTGCGCGTCACCAGTGAGGAGGGACGTTACCAATTCATTTGTCAAGACT
  GTTCTCACGTACGCAAGCCTGGACAGGTACCGGTCAGACATGATGGAAATGCTTTCTGTCTATCGACCAC
  CCGACATGGCGAGGGTGGCCGCCATCCAGTGCCTGTCACCTTCCGAGCCCGCTGCATCCTTGCCGCTCCC
  CAATGTGACCTTTGTGATTAGTCCCTCCTACGTTATTAAGGGCGTAAGTCTGACCATCACCACTACCATT
  GTTGCGACTTCAATCATCATAACAGCTATACCCCTCAATTCTACATGTGTCTCTACCAATTACAAATACG
  CCGGCCAGGACCTGCTTGTTCTGAGAAATATATCTAGCCAAACATGCGAATTCTGCCAGAGCGTGGTGAT
  GGAATACGATGACATTGATGGACCCCTGCAGTACATCTACATCAAAAACATCGATGAACTGAAAACACTG
  ACCGATCCTAACAACAATCTGCTCGTGCCAAATACCCGGACCCATTACCTCCTCTTGGCCAAGAACGGGA
  GCGTCTTTGAGATGAGCGAGGTTGGCATCGACATAGATCAGGTGAGTATCATTTTGGTGATTATTTATGT
  CCTCATTGCGATTATTGCCTTGTTCGGCCTTTACAGACTCATCCGCCTTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101489 representing NP_050229
  Red=Cloning sites Green=Tags
  
  MLFRLWVFVLLTPCYSWRPWTISDESHCKNGNSENPIVRPGFITFNFYTKNDTRIYQVPKCLLGSDITYH
  LFDAINTTESLTNYEKRVTRFYEPPMNDILRLSTVPAVKQFNLDHSIQPQIVYSLNLYPSHGIYYIRVVE
  VRQMQYDNVSCKLPNSLNELIFPVQVRCAKITRYAGENIYTHFFTPDFMILYIQNPAGDLTMMYGNTTDI
  NFKAPYRKSSFIFKQTLTDDLLLIVEKDVVDEEYRFISDATFVDETLDDVDEVEALLLKFNNLGIQTLLR
  GDCKKPDYAGIPQMMFLYGIVHFSYSTKNTGPMPVLRVLKTHENLLSIDSFVNRCVNVSEGTIQYPKMKE
  FLKYEPSDYSYITKNKSIPVSTLLTYLATAYETNVTISRYKWSDIANTLQKIYEKHMFFTNLTFSDRETL
  FMLAEIANFIPADERMQRHMQLLIGNLCNPVEIVSWAHMLTADKAPNLENIYSPCASPVRRDVTNSFVKT
  VLTYASLDRYRSDMMEMLSVYRPPDMARVAAIQCLSPSEPAASLPLPNVTFVISPSYVIKGVSLTITTTI
  VATSIIITAIPLNSTCVSTNYKYAGQDLLVLRNISSQTCEFCQSVVMEYDDIDGPLQYIYIKNIDELKTL
  TDPNNNLLVPNTRTHYLLLAKNGSVFEMSEVGIDIDQVSIILVIIYVLIAIIALFGLYRLIRLC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050229
• RefSeq ORF: 2082
• Locus ID: 1497050
• MW: 79.6 kDa