U9 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101452

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp015 [Human herpesvirus 6], codon optimized for human cell expression, NP_050190

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101452 represents NCBI reference of NP_050190 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCCGAAAGCTCTGGAAAACGGTGGTTCAGCTGTTTAGCAAGAGCAAGAGCGAAGAATGCAATA
  CCGAAGCCGGCACAATGGAGGTTTCATGCCTTAAGTACGGAGTTCAAGGTCTTAACGCCGATTGTAGCTA
  CGTTAAAAGCCAATGCATCAAGCTTAGTGAGTGCGAGTGTCTCTACACTTTCGCTTCTGATGTGTGCAAG
  GAGGACTTCCACAATAGCGAGGAGATGAAGGTGTTCGTCGTGCAACACAGCCAGGAGATTGTGGGGGGCA
  CTGACTTCTCCGTCCATGCCGAAGAATCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101452 representing NP_050190
  Red=Cloning sites Green=Tags
  
  MAVRKLWKTVVQLFSKSKSEECNTEAGTMEVSCLKYGVQGLNADCSYVKSQCIKLSECECLYTFASDVCK
  EDFHNSEEMKVFVVQHSQEIVGGTDFSVHAEESV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 312 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050190
• RefSeq ORF: 312
• Locus ID: 1497009
• MW: 11.6 kDa