U66 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101505

Myc-DDK-tagged ORF clone of viral ORF for Putative terminase [Human herpesvirus 6], codon optimized for human cell expression, NP_050241

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U66
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101505 represents NCBI reference of NP_050241 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTAGAACATGCGACATTACCCACATTAAAAACAATTACGAAGCAATCATCTGGAAAGGTGAGCGAA
  ATTGCAGTACGATCAGCACAAAGTACCCCAACTCAGCCATCTTCTACAAGAAACGGTTCATAATGCTTAC
  TCCCGAACTTGGCTTTGCTCATTCTTATAACCAGCAAGTGAAACCTCTGTATACTTTCTGCGAGAAGCAG
  CGCCACCTGAAAAATAGAAAGCCCCTGACTATTTTGCCTAGTCTGACACGCAAGCTCCAGGAGATGAAAT
  TCCTGCCAGCCTCTGATAAGAGTTTCGAGAGTCAATACACCGAATTCCTGGAGTCATTCAAGATACTGTA
  CAGAGAGCCGCTGTTCCTTCAGATAGACGGGTTTATCAAAGACTTCCGCAAGTGGATCAAAGGTGAATTC
  AACGATTTCGGTGACACTCGGAAGATTCAGCTGGAGCCTTTTCAAAAGAATATTCTGATCCATGTTATCT
  TTTTCATCGCCGTGACTAAACTTCCCGCGCTGGCCAATCGGGTGATCAATTATCTGACACACGTGTTTGA
  CATCGAATTTGTGAACGAGTCAACACTGAATACCCTTAAACAGAAGACAAATGTCTTCCTCGTGCCGCGG
  CGGCATGGAAAGACCTGGTTCATCGTTCCAATCATCTCATTTCTGCTGAAGAATATCGAAGGGATCTCAA
  TTGGGTACGTAGCCCATCAAAAACACGTGTCTCACTTTGTAATGAAGGAAGTCGAATTCAAATGCCGCCG
  AATGTTCCCTGAGAAGACTATCACATGTCTTGACAACGTTATCACCATTGACCATCAGAACATCAAATCC
  ACGGCGCTTTTCGCTTCTTGCTACAACACCCACTCAATCCGCGGTCAAAGTTTCAACCTGCTTATAGTGG
  ATGAATCACATTTCATCAAAAAAGATGCCTTCTCCACCATCTTGGGCTTCCTGCCCCAAGCCAGCACCAA
  GATTCTGTTTATATCTTCAACAAACAGTGGCAATCACTCTACAAGTTTTTTGATGAAGTTGAACAATTCC
  CCGTTCGAAATGCTGTCAGTCGTGAGCTACGTGTGTGAAGACCATGCCCATATGCTCAATGAGCGGGGCA
  ATGCAACAGCTTGCTCTTGCTACAGACTTCACAAACCAAAATTTATCAGTATCAACGCAGAGGTCAAGAA
  AACTGCAAACCTGTTTCTGGAGGGTGCTTTTATCCACGAAATTATGGGCGGAGCAACTTGCAACGTGATT
  AACGATGTATTGATCACCGAACAAGGACAAACCGAATTCGAATTTTTCCGCTACAGCACTATTAATAAAA
  ACCTCATTCCATTTCTGGGTAAGGACCTGTATGTGTACCTCGACCCCGCCTATACGGGAAACCGCCGCGC
  CTCAGGTACCGGTATCGCAGCCATTGGAACGTACCTGGATCAGTACATTGTTTACGGGATGGAACATTAC
  TTCCTTGAATCCCTCATGACCAGCTCTGATACCGCCATCGCCGAATGCGCGGCCCACATGATCCTGTCTA
  TCCTGGACTTGCACCCTTTCTTTACTGAGGTCAAGATTATCATCGAGGGGAATAGCAATCAGGCTAGTGC
  TGTAAAGATCGCCTGTATAATTAAGGAGAATATCACAGCTAACAAAAGTATTCAAGTCACTTTTTTCCAC
  ACACCCGACCAGAACCAGATTGCCCAGCCGTTCTATTTGCTGGGAAAGGAGAAAAAACTGGCGGTCGAAT
  TTTTCATCTCCAATTTCAACTCAGGCAACATAAAGGCCAGTCAAGAGCTGATTTCCTTTACAATCAAGAT
  CACTTATGATCCTGTGGAGTATGCCCTTGAGCAGATCCGAAACATTCATCAAATAAGTGTGAATAACTAT
  ATCACTTATTCCGCTAAAAAGCAGGCATGCTCTGACGATCTCATCATCGCTATTATTATGGCTATTTACG
  TGTGCTCCGGTAATAGCAGTGCATCCTTTAGAGAAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101505 representing NP_050241
  Red=Cloning sites Green=Tags
  
  MLRTCDITHIKNNYEAIIWKGERNCSTISTKYPNSAIFYKKRFIMLTPELGFAHSYNQQVKPLYTFCEKQ
  RHLKNRKPLTILPSLTRKLQEMKFLPASDKSFESQYTEFLESFKILYREPLFLQIDGFIKDFRKWIKGEF
  NDFGDTRKIQLEPFQKNILIHVIFFIAVTKLPALANRVINYLTHVFDIEFVNESTLNTLKQKTNVFLVPR
  RHGKTWFIVPIISFLLKNIEGISIGYVAHQKHVSHFVMKEVEFKCRRMFPEKTITCLDNVITIDHQNIKS
  TALFASCYNTHSIRGQSFNLLIVDESHFIKKDAFSTILGFLPQASTKILFISSTNSGNHSTSFLMKLNNS
  PFEMLSVVSYVCEDHAHMLNERGNATACSCYRLHKPKFISINAEVKKTANLFLEGAFIHEIMGGATCNVI
  NDVLITEQGQTEFEFFRYSTINKNLIPFLGKDLYVYLDPAYTGNRRASGTGIAAIGTYLDQYIVYGMEHY
  FLESLMTSSDTAIAECAAHMILSILDLHPFFTEVKIIIEGNSNQASAVKIACIIKENITANKSIQVTFFH
  TPDQNQIAQPFYLLGKEKKLAVEFFISNFNSGNIKASQELISFTIKITYDPVEYALEQIRNIHQISVNNY
  ITYSAKKQACSDDLIIAIIMAIYVCSGNSSASFREI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050241
• RefSeq ORF: 1998
• Locus ID: 1497066
• MW: 76.3 kDa