U40 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101481

Myc-DDK-tagged ORF clone of viral ORF for transport/capsid assembly [Human herpesvirus 6], codon optimized for human cell expression, NP_050221

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101481 represents NCBI reference of NP_050221 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCCTCCAAAGTCTGTGCGTTCTCTGTGCAAGACTTAATGAGTGCGCCTTGGATCTCGAGTGCC
  TCAAATTCTGTGACCCGGTTATCGTCCTGTCCGATATGGCGAACTTCAAGAAGAACGGCATCGTGATCCT
  GCATTTGTATCAGACCTTCTTTGAAGGTATTAAGGAGCAGAACCTGTTGTGTGCTAGCGCACTGACCGTG
  TACATGCAGGTGCTTTTGAAGGCCATGTATGAACAGGTGTTGTTGCTCGATGCGGCTCTCGAGTCTTTCA
  TGGTGGATCAGGATCGCAAGAAATATTTTGAGAAGGTCCTGTGTCTGAAAAGGTGTGCCGAGCATCTGTC
  AATCAACATTTCACTTAACAATGGCGTCGAATTCATTGTTCAGCTCTCCACTCTCAACGATATAGAGCAG
  CTGATTTCCAAAATTAACAGTGTGTACGCTCTGCTGTTGCCTCAGGAAGGACTTCAAATCTGTGGTAAAA
  TTATTGACTTGCTGACTATAATGTGCGGAGCCTGCATGGTGGCCAAGCCCGAATCTTACCTGGAGACCAA
  AACATGTATGAAGTGCTACGAAGAACTGACCCTCACTCCCAATCAGGGGAAGTCTCTGCGGAAAAGACTT
  CATGGCAAATTCTGTAACCACCTGACCGAACAGAAGGCCTTCTTTAATATTGAAAAGAACATAGAGACTA
  TAGAAAAGGATCTGGGGGAAGCAATCCTTAACTATGGGACCATTCAGTCCGTCGTCACCGAAATTAAGAA
  AATCTTTAAACAGCAAAGAAGCGCGGAGTCTCTGCACGTGTCCGATGCCGAAAAAACTCTGAAGAAATAT
  AACATATTCAGTAAGGTTCCAGACGTGATATACAGTCTTTCCGAATTCACTTATTGGAGCAAGATCTCAG
  AGACCATCGTGCGCAATGTAGCGATAACACTGCAGCAGCTTAATTCTTGCCACACACTTTACAAGCAACT
  TCAGAATGATGTGTCCCTCTATCTCTACGGGGAAGTGAGTGAGGATTTCCTTGCTCTCAGCGAAAACCTT
  CTCACCCACGATGAAAGACTCTACGTGGGTAGCATCTACGTGTCTCCGTCTAGGCTCATAGACCTGGTGA
  CGGGGCTGTCCATCAAGAATTTGGAAGAATCCCCCATTTTCAAAAGGCTGGCGGAGGAGGATGAGGTACA
  GCACAAGATCAAGTCCCTGCTGCACGATATCCGGGACCCACAAACGACAGAGACGCCCGGACGGCTCAAC
  ACCATCAACTGTATGCTCCAAACCCATAATCTGCAGCAAGAAGTTCTGGCCCGGAAGAAAGCTTACTTCC
  AGAAGGTGAGCGAATCCGGCTATAATAGAGTTATGGCCTGTATCCGGGAACAGGAAAGCCTCATTAACAA
  AGTAGTCTCCGTCAACGTGTATGGTAATTTCATATTTGAGGCCCTGTCAAAAATCATGAACGGATTCGTG
  CTGAGGAAGATGTACTTGGACGGCAGCATTCGGGTCGATTCCTGCACATACGACGAGCACCTTTATATTA
  AGAATAACCTTATGCCTAAAAAGCTGCCTTTGGAGCTCCTGCCTGACTTGAGCGAGATAATGTACACTCT
  CCTTACCGGTCCTCTCAGCGATTTCCACAAGTCTGCTTATCCACTGCCTGCAAATATCAGTATGGCCTAC
  GGATGCGACCACGCAGAGATGTTGCCTCATATGAAAGAAGACCTGGCTAGATGCATTGAGGGCACCATTC
  ACCCTTCAGTCTGGATGGTCTGCGAATACAACGAATTCTTCAACTTCTCCGGCGTGACAGACGTAAACGA
  CATGCAAAAAAAGATGTGGAATTTCATTAGAGAGTTGACCCTCTCTGTAGCTCTCTACAATGACGTGTTC
  GGCAAGCGACTCAAGATTGTGCGCATTGACGAGGAAGAAGACCTCAACGGCAATGTGGTGCTCACTTTCA
  ACCACGAATCCCCCCTGCTTTTTCATACTGGAGGCGGTATGACTAAGTTTAAAGACGTGTATTCCTTGCT
  CTACTGCGATCTGCAGGCCCAGCTCAGCAGAGAGACGGTGGACGTACCAGAAGGCGTTTCATACTCCGTC
  CGCACTCCAAACCTCCTGGACTTGGTTCGAGAGAACGAGCAGGACGAAAGCATTATTCCTGGGTGTTTGT
  TCGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101481 representing NP_050221
  Red=Cloning sites Green=Tags
  
  MNSLQSLCVLCARLNECALDLECLKFCDPVIVLSDMANFKKNGIVILHLYQTFFEGIKEQNLLCASALTV
  YMQVLLKAMYEQVLLLDAALESFMVDQDRKKYFEKVLCLKRCAEHLSINISLNNGVEFIVQLSTLNDIEQ
  LISKINSVYALLLPQEGLQICGKIIDLLTIMCGACMVAKPESYLETKTCMKCYEELTLTPNQGKSLRKRL
  HGKFCNHLTEQKAFFNIEKNIETIEKDLGEAILNYGTIQSVVTEIKKIFKQQRSAESLHVSDAEKTLKKY
  NIFSKVPDVIYSLSEFTYWSKISETIVRNVAITLQQLNSCHTLYKQLQNDVSLYLYGEVSEDFLALSENL
  LTHDERLYVGSIYVSPSRLIDLVTGLSIKNLEESPIFKRLAEEDEVQHKIKSLLHDIRDPQTTETPGRLN
  TINCMLQTHNLQQEVLARKKAYFQKVSESGYNRVMACIREQESLINKVVSVNVYGNFIFEALSKIMNGFV
  LRKMYLDGSIRVDSCTYDEHLYIKNNLMPKKLPLELLPDLSEIMYTLLTGPLSDFHKSAYPLPANISMAY
  GCDHAEMLPHMKEDLARCIEGTIHPSVWMVCEYNEFFNFSGVTDVNDMQKKMWNFIRELTLSVALYNDVF
  GKRLKIVRIDEEEDLNGNVVLTFNHESPLLFHTGGGMTKFKDVYSLLYCDLQAQLSRETVDVPEGVSYSV
  RTPNLLDLVRENEQDESIIPGCLFDE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050221
• RefSeq ORF: 2178
• Locus ID: 1497042
• MW: 83.0 kDa