U15 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101540

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp021 [Human herpesvirus 6], codon optimized for human cell expression, NP_050196

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101540 represents NCBI reference of NP_050196 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTATGGAAGAGACAGCGACTCCAGGAGTGCAGAGAGTTGTGCCCACTGCCCGCTCTTATGTCCC
  TTAGCAACATCCTTTCCAATACAGAAATTATTTACGTAAAATATTTGTTTAAGATGGATTTCTCTACAAT
  GTACCGATTCATTCTGCCCGCTCTCACCCTGTCCATGACCGTGACCAAATCCGTGGTCATCGAGATGCTC
  TTTATTCTCAAACGGTGGGAAGAGATCAATCAGTTTTTCAGACTGAACATCCGAAAGGTCAATGACTGCT
  TCGTGGTTGCCCAGTTCACCAATATACCCGTGAAAAGAAAAATCATTGTGCTGCTCTATATGCTGACAAG
  CAGACAGGAAAAGCAGCTGTTCCTGAATATGATTTACGCTTTTCTCGAAAAATCTCACCTGAGGCTCGGG
  GACGATGAAGAACAGAACGCGATAAGGTTTTTTAGCTACGTCGACGAGCTCCACCTTACCCGCGATGTGC
  TTCTTGAGATTATTTACAAGCTGAAAAATACTGAAATAAATCAGACTATGGAATTGCTGCTCTCCTACAA
  CGAATTGGCCGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101540 representing NP_050196
  Red=Cloning sites Green=Tags
  
  MDVWKRQRLQECRELCPLPALMSLSNILSNTEIIYVKYLFKMDFSTMYRFILPALTLSMTVTKSVVIEML
  FILKRWEEINQFFRLNIRKVNDCFVVAQFTNIPVKRKIIVLLYMLTSRQEKQLFLNMIYAFLEKSHLRLG
  DDEEQNAIRFFSYVDELHLTRDVLLEIIYKLKNTEINQTMELLLSYNELAG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050196
• RefSeq ORF: 573
• Locus ID: 1497101
• MW: 22.9 kDa