U27 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101468

Myc-DDK-tagged ORF clone of viral ORF for Polymerase processivity factor [Human herpesvirus 6], codon optimized for human cell expression, NP_050208

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101468 represents NCBI reference of NP_050208 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGGGGTCTCGCGATCATCATCGCGACCATCGAGATCATAGAGAGCACCGAGAGCTCAGGGAAC
  CTCCTACTCTTGCTTTCCACATGAAGTCCTGGAAGACCATCAACAAACCCCTGAAGGCATTCACCAAGCT
  GTTGAAAGAGAATACAACAGTTACTTTCACTCCTCAGCCTAGCATAATAATACAGTCCGCCAAGAACCAT
  CTGGTGCAGAAACTCACAATCCAGGCTGAATGTTTGTTTCTGTCTGATACAGAGCACTTCCTCACCAAAA
  CCATTAATAACCATATTCCACTGTTCGAGTCTTTCATGAACATCATATCAAATCCCGAGGTGACCAAACT
  GTACATTCAGCACGACAGTGACCTTTACACTCGAGTGCTGGTAACCGCATCCGACACTTGTACTCAAGCA
  TCCGTGCCCTGCGTGCACGGACAAGAGGTGGTTCGGGACAGCGGAAAGAGCCCTCTTAGAATCGACCTGG
  ATCATTCCACCGTATCTGAGGTTCTGAAATGGCTCTCTCCGGTCACAAAGACCAAACGATCCGGAAAGAG
  CGACGCCTTCATGGCTCATATTATCGTACAGGTCAATCCACCCACCATCAAGTTTGTGACGGAAATGAAC
  GAGCTCGAATTCTCCAACTCTAACAAAGTTATCTTCTATGATGTTAATAATATGAGATTTAACCTGAGTG
  CAAAGAATCTTCAGCAGGCCCTGTCAATGTGTGCTGTTATCAAGACATCCTGTAGTCTCCGCACAGTGGC
  TGCCAAGGACTGCAAGCTGATCCTGACGTCAAAGTCAACTCTGCTCACTGTGGAGGCGTTTCTTACCCAG
  GAGCAGCTCAAGGAAGAGAGCAGGTTCGAGCGCATGGGCAAGCAAGACGACGGGAAAGGGGACCGCAATC
  ACAAAAACGAAGATGGCTCAGCTTTGGCTTCTAAACAGGAGACCCAATATAAAATTACAAACTATATGGT
  GCCCACGAAAAATGGTACCGCTGGGAGCTCACTGTTTAACGAAAAAGAGGATAGCGAAAGTGACGACAGT
  ATGCATTTCGAGTATTCTTCCAACCCCAAACGGCAGCGCTGCGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101468 representing NP_050208
  Red=Cloning sites Green=Tags
  
  MERGSRDHHRDHRDHREHRELREPPTLAFHMKSWKTINKPLKAFTKLLKENTTVTFTPQPSIIIQSAKNH
  LVQKLTIQAECLFLSDTEHFLTKTINNHIPLFESFMNIISNPEVTKLYIQHDSDLYTRVLVTASDTCTQA
  SVPCVHGQEVVRDSGKSPLRIDLDHSTVSEVLKWLSPVTKTKRSGKSDAFMAHIIVQVNPPTIKFVTEMN
  ELEFSNSNKVIFYDVNNMRFNLSAKNLQQALSMCAVIKTSCSLRTVAAKDCKLILTSKSTLLTVEAFLTQ
  EQLKEESRFERMGKQDDGKGDRNHKNEDGSALASKQETQYKITNYMVPTKNGTAGSSLFNEKEDSESDDS
  MHFEYSSNPKRQRCVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050208
• RefSeq ORF: 1098
• Locus ID: 1497029
• MW: 41.7 kDa