U7 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101461

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp013 [Human herpesvirus 6], codon optimized for human cell expression, NP_050187

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101461 represents NCBI reference of NP_050187 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAATTTCCGCCTGATCCTCGAAACAGAGGGGGGTAAAAGGGTGTTGTGTGAGAGCATCGTGCTTC
  ACTTTGCCTTCATGTTTCAGACTGAGATAGTGCCCGTATGTAACGATGAGTTTACTCTGAGCAGCTGTGT
  GCCGACTCTCGACTTTGACGTGGATGTGTTGTCAGCCGCCTATGGGGACGGTCTGGAAATCTCTTCACCC
  GGCCTGCGATGTTGCATTGCATGGCCGCCAATGTATGCCTTGACTCTTGGCGAGTTCTACCACTTCCACA
  CGCATCGATGGGTCTCCGCATACGACTGGTCTAGCCTCCTGGATACTGATGAGTTTTTGAGCGCCATCGG
  CTACGCTCACCCTATTTATAGAGATCCCAATCCAGATTATGACCCCTACGTTATGCACTCTTCCACTGGA
  AAAACCATTGCCGTCGATACCGTCACTGAGAAGGTGTATATCATAGCAGAATCATTGGTGCAGTTCTTCA
  ATATCGGACTCAGACAGTTCCCCCCTTTTGCCGAGGCTGAACTGGACCCCGAACAGGAAAAAATGTGGTT
  CGGCGAAACGAAATGTGGCAGGGAAGAGTTCATCCTCCTGCAGCGGAATCTGCCTGCCATGAAAGACTAT
  GTGGCTAAACACTGTGGAAAGAGGATCAGGGTGGATGCTTTCCAGGATTTCGACTTCAGTTTCTGCAGTC
  TGAGCGACATTTACTATTTGACTGGGCCTGGAATACTGGAGAAAATCACCGAGAAGGACTATGCAATCAT
  CGGGACTTGTGCCCGAAGCCAGGCCGAGCCCAACTGCCGCGCCGCAATCGTAATGGGCAGCAATTGTCAT
  ATATATATTTACGTGGAAAATCGGATCTCTAAAGTCTCAAAAAGCCTCCGGACCTTTATCCGCCGCGGAT
  TCGATGAGCTTCTCTATAAAGAAAAGTATTCCCTGGACTGGAACGACGATACACTGTTCTACATCTCAGA
  TACCGAGACTGAAAATCTGAACCGAATGCTGAATGGAGAGCTCCCAGTCCTGCGGAGCAAGCCTCGACAC
  ATGTGCGTTAGGAAGGATCGCCTTGTGAAGGATAGGTCCAAAATTCTGTTCGCCGTGAGGTTGGATGAAG
  AGGATAGCCTCACGGTTAAGTTCATAACAAAGTTTTTGACTCCCGTCTTCGTGGGCCGACTCCCGGCAAC
  GTCCCGATTCGTAGTGCCAGTCTCCTGCGCCAGACTGACGAATGGACTTCAGGGCACGGCCGCAGCCCGG
  TTCGGGATCAAGGGCCTGCACCCGTCATCTGATTGCGTGGTCTGGAATCGCTTGATTGATTATGAATACG
  AGACTTATAAGTATCCTAGCACCTACGTCCGGGCAGACCAGATTGCGGATATGGTAAAAAACCTGAAGTT
  CATGGATAATTTCGATGAAAAGTGGCAGTGCATTACCAAATTGGCATTCATTGGACTCTACGCTGGGGCC
  AGCCTGTTCAATTTCGCCAGCAAACCGACCCTTGGCTACTGGTGCCGGTACCTTTGCGAATATGCGTCAA
  TGCTGCTTTTCCAGTTTGAGTCAAAGCTGAAGGAACTGACAAAGGAATCAACCCGACAGCTCGGAGGGTA
  TAACCTTTGCCACTGGGGGCAGGAACTGAAAGACTGCCTCGAAAACAAGAGTGACGTATTTTTCCGGTAC
  GATTTTTTTGAGCGAATAGAGTCTTGCTTGATAGAACATTTTATGCTCTTGTGCGGCTGTGTCGAATGTC
  GACGCATGTTCATCATGTATAATAAAAGGGGGCGGAAGTGTGATTTTGGCCATTGTGTTCGGATCCAGTG
  CTTCCCTATGATCGGCAGTATTCGACTTCCAGCGTTTCTTCACCTCGGGGAACCTTACTCCGTGTCCCTC
  TCCAGCTTGATTGCCAAAGACCTGGGCCTGTCTATGATAGAGGGCCAGATAGAGCATAGTAGATTGCCCA
  TCAGTCTGCAAATATCTGTTACCCCTGACAAGAAAGCGTTGCTTACCTTTTTGACCAATATCGTGTTTAT
  CGTGTTCGTCGTCAACACACTTTATCGGGTGATCAATGCAGAGCTTGACATCTACTACGACCTTTTCACC
  GAAGAGGTGGGCAAGCTCTGCGTTACAATGGAAGAAGAGATGAAGCTGGGGAGAAACGGCTGTCTGGGTG
  ATCTTTGTTATTTTTCCAGCATGAAACAAATGAAGGAGATAGTGCGGTGTCCAGGGGAGAAGTCCCAATT
  CATTCTGAAGTGCTGGGAGGCGCTGAGGATCGGGTTTTCCGTGCCCGCATACAAGGATTATGACGAAACG
  AGATTCATGGAGATGTTTTTCCTCCACCACCTGCACATTAAAAGGTTCCACGAGCACAATGACCGCGATT
  TGGTGTCTAGTGATAATCTGATCCCGGGGTTTTTCATCGTGAACACCCACGAAGAGAACTTTCTTCAGCG
  ATTGCAACGGGTTGTCCTGCCTGTAGCCGAAGACTACCTTACAAACACACGGTGCATTAATGGAACGATG
  GCTTTCTTTTTTAGCGGGCTGAAGTATTTTGGGTCTGGGAACCATAGGGGTGTACAGATCTCCCCCGAAA
  AGGACGTGAGGGCAATTGGCTACAAACTTGGATCATTGGACGTGCTGCGAGACGACTATAAGTACTACGA
  ATACGCCCCTGACTGTGCCGGCGAGCTCAATGGACACGACGGTGACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101461 representing NP_050187
  Red=Cloning sites Green=Tags
  
  MSNFRLILETEGGKRVLCESIVLHFAFMFQTEIVPVCNDEFTLSSCVPTLDFDVDVLSAAYGDGLEISSP
  GLRCCIAWPPMYALTLGEFYHFHTHRWVSAYDWSSLLDTDEFLSAIGYAHPIYRDPNPDYDPYVMHSSTG
  KTIAVDTVTEKVYIIAESLVQFFNIGLRQFPPFAEAELDPEQEKMWFGETKCGREEFILLQRNLPAMKDY
  VAKHCGKRIRVDAFQDFDFSFCSLSDIYYLTGPGILEKITEKDYAIIGTCARSQAEPNCRAAIVMGSNCH
  IYIYVENRISKVSKSLRTFIRRGFDELLYKEKYSLDWNDDTLFYISDTETENLNRMLNGELPVLRSKPRH
  MCVRKDRLVKDRSKILFAVRLDEEDSLTVKFITKFLTPVFVGRLPATSRFVVPVSCARLTNGLQGTAAAR
  FGIKGLHPSSDCVVWNRLIDYEYETYKYPSTYVRADQIADMVKNLKFMDNFDEKWQCITKLAFIGLYAGA
  SLFNFASKPTLGYWCRYLCEYASMLLFQFESKLKELTKESTRQLGGYNLCHWGQELKDCLENKSDVFFRY
  DFFERIESCLIEHFMLLCGCVECRRMFIMYNKRGRKCDFGHCVRIQCFPMIGSIRLPAFLHLGEPYSVSL
  SSLIAKDLGLSMIEGQIEHSRLPISLQISVTPDKKALLTFLTNIVFIVFVVNTLYRVINAELDIYYDLFT
  EEVGKLCVTMEEEMKLGRNGCLGDLCYFSSMKQMKEIVRCPGEKSQFILKCWEALRIGFSVPAYKDYDET
  RFMEMFFLHHLHIKRFHEHNDRDLVSSDNLIPGFFIVNTHEENFLQRLQRVVLPVAEDYLTNTRCINGTM
  AFFFSGLKYFGSGNHRGVQISPEKDVRAIGYKLGSLDVLRDDYKYYEYAPDCAGELNGHDGDE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050187
• RefSeq ORF: 2709
• Locus ID: 1497021
• MW: 104.2 kDa