U29 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101470

Myc-DDK-tagged ORF clone of viral ORF for capsid assembly and DNA maturation [Human herpesvirus 6], codon optimized for human cell expression, NP_050210

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101470 represents NCBI reference of NP_050210 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCTAAGTCTAGTGCTAGAGCGGCCATCGTGGATACCGTCGAGGCCGTGAAAAAAAGAAAGTACA
  TCTCTATAGAAGCCGGTACCCTCAATAACGTAGTGGAGAAAGAGAGAAAATTCCTTAAGCAGTTCCTGTC
  AGGCAGGGAAAACTTGCGCATCGCCGCACGGGTGTTTACTCCGTGTGAATTGCTTGCACCCGAACTGGAG
  AACCTGGGGATGCTGATGTACCGGTTCGAGACTGACGTGGACAATCCAAAGATTCTTTTCGTCGGTCTGT
  TCTTTCTGTGTTCAAATGCTTTCAACGTGTCTGCTTGCGTCAGGACCGCTCTGACTACAATGTATACTAA
  CAGTATGGTTGACAATGTCCTCTCTATGATCAATACCTGTAAGTATCTTGAGGATAAGGTCTCACTGTTC
  GGCGTGACATCTTTGGTCAGTTGCGGCTCTAGTTGTCTCCTGAGTTGCGTGATGCAGGGCAACGTCTACG
  ACGCTAACAAGGAGAACATACATGGCTTGACTGTGCTCAAGGAAATTTTCTTGGAGCCCGACTGGGAGCC
  CCGACAACATAGTACCCAGTACGTGTACGTGGTCCACGTGTACAAGGAGGTGCTGAGTAAGTTGCAATAC
  GGGATTTACGTGGTTCTCACATCCTTCCAGAATGAAGACCTCGTTGTGGATATTCTCCGGCAATACTTTG
  AGAAGGAGAGATTTCTGTTCCTGAATTATCTGATCAATTCTAATACTACACTGTCCTACTTCGGAAGTGT
  ACAACGCATAGGAAGATGCGCCACAGAAGACATCAAGTCCGGCTTCCTGCAATACCGAGGTATTACGCTG
  CCTGTCATCAAGCTGGAAAACATTTTCGTCGATCTGTCTGAGAAAAAGGTGTTCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101470 representing NP_050210
  Red=Cloning sites Green=Tags
  
  MNSKSSARAAIVDTVEAVKKRKYISIEAGTLNNVVEKERKFLKQFLSGRENLRIAARVFTPCELLAPELE
  NLGMLMYRFETDVDNPKILFVGLFFLCSNAFNVSACVRTALTTMYTNSMVDNVLSMINTCKYLEDKVSLF
  GVTSLVSCGSSCLLSCVMQGNVYDANKENIHGLTVLKEIFLEPDWEPRQHSTQYVYVVHVYKEVLSKLQY
  GIYVVLTSFQNEDLVVDILRQYFEKERFLFLNYLINSNTTLSYFGSVQRIGRCATEDIKSGFLQYRGITL
  PVIKLENIFVDLSEKKVFV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050210
• RefSeq ORF: 897
• Locus ID: 1497031
• MW: 34.1 kDa