U50 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101491

Myc-DDK-tagged ORF clone of viral ORF for viron protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050231

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101491 represents NCBI reference of NP_050231 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGTGCAATCTGTTCTATCAGTATCCAATTACACCCATACTCGAGGGCCACGTGCGAAACATCT
  TGATTTGTACTGAAGAGGACATTAGGAGACTGCAGTCCCAGTCCAGCCTTAGACTGAGAGAGAAAATCGA
  TCAGGGACATAGGGATAAACTGCTGCGGATGAGGCTGAAAACAGAGCTGGACGCGCTCCAGCGGAAAATG
  CAGAAGGACAGCGATGTTCTCAATTCCCATTTGAAGGCCATTGAGGATGCCCTCCTGTTCACTAACGATG
  GAGAGGTGAATGTTGAGACTAAGGCGGACACACAACTTCTGCCCAAAAGTCCCGAAAGATTGGAAAAATT
  TAATCAGGTGGCCATTACCCCACTGGACCCTTTCATTAGATTTACCGACGATTTTCGGGGCGAGATGATC
  AACACCTTTTTCAACAACGCTCAGATGTGGAATTTCACCTTTGGCAGTTGGTTTTACAAGCTTAAGCGGG
  TTTTCTACAATGAACCTGGTTTGCGAAGAGCTCTTAAGATTACTAACGTGGACAGCTTGACCATTTCTAA
  AGAGCTGCTCACTGTTACAGTCAACGCTCTCGAGCAAGCCACAGTGTATCCGATATTTGGCAGTGAGATG
  AGCGATCTGGAGGCAGCCCTTTGCATCCTCGCAGCTTTTTATAGTACCTATGAGAATAGCCAGATCGATG
  AGAGAACAACGCTCGTTGACATCATCACTCTCCTGCCGGTGATCTTTAGACTGCTTGGATCAGAGATCAC
  GGCCCTTAAGAATGTATCACCTTCAGGCACCTATTTCGGGTTCAACGATCCCTCCTGTATGAAGTTTTTT
  GTACCAATGCGGAAGGGTAAACACTACGCAGAGAATACGTTTGGGAATCATGTGCTGGTTAAGATGTTGC
  TTGGCAGAGGCGTGATGCAGAAGATTCCCGGAGAGAAGGATTCACAGAATTTCGATGTGGAGGCCCGACT
  GCATGGTGCCATTAAAAACGACGTACTGGTGTATTGGACTTACCAGCTGATGAGGCCTAAACTCGGCAAC
  AATGTTCCCATATTCATCCATGACCAACATTATCTCCGGTCAGGGCTGGTCGCCATAGAGTCCCTGTTCC
  TGCTGTGGAGGATCTTGAATTCTGAGTCCCTGTTCAACAAGCGGGTGGGAAAATTTCTTCTGACCTCAAT
  ATTCCCACAGCTGGAAAACGTCGACTTCGCCGAGAACAATTTTGAGGCCGGTAATATACAGAATTTCGAA
  TACCTCATGCATCACTATGTTGTGCCAATGTACAACCTGCAGAACGATATTTCAATTTCAACACTTTTCC
  CAGGGCTGGTGGCAGTGTGCGTTAATGAATCTGTGAGACTGGGCTGGGAGCATAAGTGCGCGGGTGCGCC
  TTCCGACGCCGTGCAGGTACAGTCAAAGGAAAATCCATTTGTTGAGTATATACGAGCCCAGATGGAACAA
  CAGGCCGACGTCGCCATCCTCGAGAAACACGATTGCATCCTCTTCCATTTTGAGAATGGGCTCAACATCA
  CCCTTAGCTTCACCCTCCCTCGACAGCGGCTCTTCGCTATGGCCTCTTCCCTGTTCAATGTTAATGATAC
  CTATGATTTCATCTATTTCCTCGTGCTCGGCTTCTTGCCCATCCCTGCCATTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101491 representing NP_050231
  Red=Cloning sites Green=Tags
  
  MAQCNLFYQYPITPILEGHVRNILICTEEDIRRLQSQSSLRLREKIDQGHRDKLLRMRLKTELDALQRKM
  QKDSDVLNSHLKAIEDALLFTNDGEVNVETKADTQLLPKSPERLEKFNQVAITPLDPFIRFTDDFRGEMI
  NTFFNNAQMWNFTFGSWFYKLKRVFYNEPGLRRALKITNVDSLTISKELLTVTVNALEQATVYPIFGSEM
  SDLEAALCILAAFYSTYENSQIDERTTLVDIITLLPVIFRLLGSEITALKNVSPSGTYFGFNDPSCMKFF
  VPMRKGKHYAENTFGNHVLVKMLLGRGVMQKIPGEKDSQNFDVEARLHGAIKNDVLVYWTYQLMRPKLGN
  NVPIFIHDQHYLRSGLVAIESLFLLWRILNSESLFNKRVGKFLLTSIFPQLENVDFAENNFEAGNIQNFE
  YLMHHYVVPMYNLQNDISISTLFPGLVAVCVNESVRLGWEHKCAGAPSDAVQVQSKENPFVEYIRAQMEQ
  QADVAILEKHDCILFHFENGLNITLSFTLPRQRLFAMASSLFNVNDTYDFIYFLVLGFLPIPAII
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050231
• RefSeq ORF: 1665
• Locus ID: 1497052
• MW: 63.8 kDa