U19 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101458

Myc-DDK-tagged ORF clone of viral ORF for immediate-early protein 4 [Human herpesvirus 6], codon optimized for human cell expression, NP_050199

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101458 represents NCBI reference of NP_050199 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCACCGGAGACGAGCTGGCGAGAATGCTGCAGTTCAAGGATAAGATGATCAGCTCAGCCGGGT
  CTGCCCTCCGCTTTGAGAAGGTGGTCCAAGAAGCTATGGCCAGCGGCATTGTTCTGCAGCACATCACATG
  CATTAAGGTGCGCATTTGCGATAATAGCGATATTCTTTCAGACAGGCAACTGAGGTGTCTCCTGATCAAC
  GGGCTGTATCCCTTCGAAGGTCGGATGAGTATGTTCGGCGTGACGGAGGAGTGGGAGGGCGCCTCAGCTG
  CCCCCGAGAGGCAGGTCGTGTTTCTGCTCAGCTCTACAGGCCAAGTGCTCGGATACGAAGACGGTGTTAT
  TTTTTATCTGTCCCCCACCTTCTCCGATTTCTGGACCACCGCTATGGAGTTTTCATGTCAAAATGCAATT
  CTTTCAAATTTCATCGCACAAAAGTCACGCGACGAATACTCCGATCAATTCCAGAAATATTTCACCAGGA
  TGAGACACACCCCGATAAGCCTCACAGGTGTTCTCCCCCGCAGATTCCAGAAAGTTGAGTCAGGCGCTTG
  TGTGGAAGAAGATGCGAGAGCCTCTATGCGGCCCATACAGAGCGACTCCTTTGGTGCCAAGGGACCGTTC
  TGCTGGCCTACAGAAGAGCTTCTCCAACCATCTGCCAAGAAAGATGTGGGTGGCACTGTTTGTATGGCAT
  TGTCCTGTCAAGAAGATAACTCTGCGAGACACTGTACCATATACGGGTTGACAAAGACGCCTGGCATCAA
  GATAATGCTGTCCCGGCACACCCAGACGGACCGAAGCGAAGCAATGTGCGATGCTGCCACTCAAACAGAA
  GATGTAGTGGACAATTCAAGCGAAACGCTCTTTTTGGGCGGCAATTTGGTTCATCAATCAATCTTGGAGA
  CCGAGGTACAGGCTACAGCCAAGAACACCTTCGACGTTAGCGATCCTCGCATTGATAGTGTGTACGACAC
  TACGGTAGTTGGCGCTATGGCAACTGACGACGTAGGATGCAAACATGTCCAGGGAGGAGCCTCCCTTGCA
  CAGGAAAAACCACTTAAAGGTTATTGTATCATCGCGACCCCAAGTGAATGCAAGCCTAATATACACTGGC
  TCAAATCTCCTGAAAACGCTGTACACGAATCCGCCGCCGTGCTGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101458 representing NP_050199
  Red=Cloning sites Green=Tags
  
  MAFTGDELARMLQFKDKMISSAGSALRFEKVVQEAMASGIVLQHITCIKVRICDNSDILSDRQLRCLLIN
  GLYPFEGRMSMFGVTEEWEGASAAPERQVVFLLSSTGQVLGYEDGVIFYLSPTFSDFWTTAMEFSCQNAI
  LSNFIAQKSRDEYSDQFQKYFTRMRHTPISLTGVLPRRFQKVESGACVEEDARASMRPIQSDSFGAKGPF
  CWPTEELLQPSAKKDVGGTVCMALSCQEDNSARHCTIYGLTKTPGIKIMLSRHTQTDRSEAMCDAATQTE
  DVVDNSSETLFLGGNLVHQSILETEVQATAKNTFDVSDPRIDSVYDTTVVGAMATDDVGCKHVQGGASLA
  QEKPLKGYCIIATPSECKPNIHWLKSPENAVHESAAVLR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050199
• RefSeq ORF: 1167
• Locus ID: 1497015
• MW: 42.9 kDa