U58 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101499

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp064 [Human herpesvirus 6], codon optimized for human cell expression, NP_050239

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101499 represents NCBI reference of NP_050239 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCACACCGGTAATTGTGAGACCCTCATCGTGAACTCATGCTTTGGATCCACATGCGCTAGATCCA
  TACCTGTCTTTATCGACAGCTGCGACCTCACTGCCGAAGTATCAAGAGATGAAGAAACGCGCCTCGCCAG
  AAGTGTCCCAGTCGTGCTCGAGAAAATTGAATCAATTATCGAGAAGATTTTCCAGACCAGCGGACCCAAC
  ATCGTTCATGACAAAGATAGGGCGAAAATTGCACTCTGTCGGTTGCTCCTCGGGCCTGTGGCTGTACCAT
  GCTTCTGCGAAGAATGGGATACCAATGACTATCTCAGTAAGTCTGGCTGTAAATGCCTGGGACCAATACT
  TTATATCCACACTTCCCGCTGTCGCTGTTCTGACATTCCCGTATTTAAGTTCAGCATCATGAAAGACTAC
  TATGCTTCTCATGTTTTTCGAGGACTGCTCTCTCTCAAGGAGTGGAATACCCACCTGCCAAATGTGCTGT
  GTACTTGCGAACTGAGTATGAGTGATAGGTACGTCGCTACAGTGTTCCCCAAGCAGAATAGCATCTATCT
  GGAGTACTATCCTTATTTCCTGTGCTATCTGTGCAGGTATCTGACGGTCATCGAAATTGAGCAATGCACT
  AACGACCTGATTAGCCTGCTGGGACCAAAAGTGGCTCAACGGGTAATCATACACTTTAAGCTGCTTTTTG
  GTTTCAGGCATAAACCCCACATGGGCACCGTCGACTCCTGGTTTTGGGAAAATTTCTTTATGCTTGAGCT
  GCACAAGCTGTGGCTCACTGTGGTTAAACACAATAGAGTGACCACAGACTTCTTTAACGTGGTGTACGAG
  AAGATTCAGAATTATAAGCAATATGCCATAAAAACGCTGAGAATGAGTTCCAAAGCGGTACCTGCTATTC
  AGCGCTTCTGTCTGGCCAAATTCAAGCAGCAGCTGCTGTACCTGAACATTAAAGTGACCGTAAAAAAGAA
  TAAGCGCGAGTTGTGTCTGAACGGATTCGTGTACGGCAAAACACTTTATGTGGTGGAGAGTAGCCAGCTC
  ATCTTCCGGAACCTGTTGTTGCTTTATTACGACTACTCCCTGCCCGATGAGTGCAAAACTAAAGAGGAGA
  ATGTGCTCACCACACACTACATCAGGGTCATATCCAGGTTGTCCTTCAAGCAAAGCCGGTCCGCCGTCCC
  GCCCGGAGTCAAGCCCGACTTCACATTTGTTGCCCAGTTGCCTAAACGAAAGGAACTCCCAAATGTTCCA
  GGCGGAATTGATTTTGCCGAGATCACCTCCGTGCGGCACGGCGCAGTTATCCTCAATGCATTCAACACAA
  ATAAAGTCATGAATCTCAAAGCCACAATTAGCAAAAGGGCGAATTTCGTGTATCACCGAATCCCTAAGAC
  TATGACCCACAGTTTTGTCATGTACAAGCACACTTTCAAGGAACCAGCATTCACCGTGTCCACCTTTGTG
  TCTAACGACGACCTGGACATGTCCAGCCTGAATATCAACATCCGGGGACCCTATTGCGACTTCCTGTATG
  CTCTGGGGGTTTATAAGATGCATGTTTCCATTCAGGATCTGTTCCTCCCAGCATTTGTGTGCAACTCTAA
  CAATTCCGTCGACCTGCAGGGCTTGGAGGACCAAGATGTCGTCCGGAACAGAAAGAAGAAGGTCTACTGG
  ATAACAAATTTCCCCTGTATGATTAGCAATGCAAACAAAGTCAATGTCGGATGGTTTAAAGCAGGCACTG
  GAATCATCCCCAGGGTGAGTGGCAAAGACTTGCAGAACGTGCTTCTTCAGGAGCTCAATAATGTACGCGA
  GATCCCTGGGCTCGTGTTCGATATGGATCTCCACCAATTGCTCGTCCTGCTGGAACAGCGAAACTTGCAC
  CAGATACCATTCCTTGTCAAACAATTTCTGATTTTTCTCCGGCTGGGCCTCCTTATGGGTTACGGAAACT
  CAAGGAGGAATAAAGTGCACGACATCATGCTCCACCTTATATCAAACGGTCTGTTCGACTTTAATAAGAA
  CAGCGTCGCTAACACCAAAATCAAGCATGGCTGCGCACTTGTGGGCACCAGACTCGCGAACAATGTGCCT
  AAGATAATCGCCCGCCAGAAGAAGATGAAACTGGATCATATGGGGAGGAACGCCAATAGTCTCGCCGTGT
  TGAGATTCATCGTCAAATCCGGCGAACATAAGAACAAGACAGTTTTCATTAAGCTCTTGGAGTATCTCGC
  AGAGACCTCTACTGCTATCAATACCCGAAATGAGGTCGCTCGCCTGCTGCTGACCCTTACCACTAACATG
  AAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101499 representing NP_050239
  Red=Cloning sites Green=Tags
  
  MQHTGNCETLIVNSCFGSTCARSIPVFIDSCDLTAEVSRDEETRLARSVPVVLEKIESIIEKIFQTSGPN
  IVHDKDRAKIALCRLLLGPVAVPCFCEEWDTNDYLSKSGCKCLGPILYIHTSRCRCSDIPVFKFSIMKDY
  YASHVFRGLLSLKEWNTHLPNVLCTCELSMSDRYVATVFPKQNSIYLEYYPYFLCYLCRYLTVIEIEQCT
  NDLISLLGPKVAQRVIIHFKLLFGFRHKPHMGTVDSWFWENFFMLELHKLWLTVVKHNRVTTDFFNVVYE
  KIQNYKQYAIKTLRMSSKAVPAIQRFCLAKFKQQLLYLNIKVTVKKNKRELCLNGFVYGKTLYVVESSQL
  IFRNLLLLYYDYSLPDECKTKEENVLTTHYIRVISRLSFKQSRSAVPPGVKPDFTFVAQLPKRKELPNVP
  GGIDFAEITSVRHGAVILNAFNTNKVMNLKATISKRANFVYHRIPKTMTHSFVMYKHTFKEPAFTVSTFV
  SNDDLDMSSLNINIRGPYCDFLYALGVYKMHVSIQDLFLPAFVCNSNNSVDLQGLEDQDVVRNRKKKVYW
  ITNFPCMISNANKVNVGWFKAGTGIIPRVSGKDLQNVLLQELNNVREIPGLVFDMDLHQLLVLLEQRNLH
  QIPFLVKQFLIFLRLGLLMGYGNSRRNKVHDIMLHLISNGLFDFNKNSVANTKIKHGCALVGTRLANNVP
  KIIARQKKMKLDHMGRNANSLAVLRFIVKSGEHKNKTVFIKLLEYLAETSTAINTRNEVARLLLTLTTNM
  KT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050239
• RefSeq ORF: 2316
• Locus ID: 1497060
• MW: 88.7 kDa