U68 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101507

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp072 [Human herpesvirus 6], codon optimized for human cell expression, NP_050247

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101507 represents NCBI reference of NP_050247 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTGAAAGATTACCTTAGGCAGTCCATCTCTAAGGATCTGGAGGTGCGACACCGGGACTCTTTGA
  AGATCAGACTTGGGGAGCGGCACCCTCTGTCCGTTCATCAGCATATGATTGCCGCCCGGCAAATAATTAA
  GAGCGACAATGCCGAACACCAGCACGTTATCTCTAGTCTGAGTGGCTTCCTGGACAAACAGAAGAGTTTC
  CTCAAAGTCCAACAGCGCGCTCTGAAGCAGCTGGAGAAACTGGATGTAGACGAAATCATTGACACCGCAG
  CTGAAGTTAAAGCGGTCTCTAATAACATCAAAGAAACCCTTATGGCCTCCACCGAACTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101507 representing NP_050247
  Red=Cloning sites Green=Tags
  
  MSLKDYLRQSISKDLEVRHRDSLKIRLGERHPLSVHQHMIAARQIIKSDNAEHQHVISSLSGFLDKQKSF
  LKVQQRALKQLEKLDVDEIIDTAAEVKAVSNNIKETLMASTELE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 342 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050247
• RefSeq ORF: 342
• Locus ID: 1497068
• MW: 13.1 kDa