U69 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101508

Myc-DDK-tagged ORF clone of viral ORF for Phosphotransferase [Human herpesvirus 6], codon optimized for human cell expression, NP_050248

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101508 represents NCBI reference of NP_050248 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAACGGGGTAGAAACACCACAGGGACAGAAGACACAGCCCATCAACTTGCCCCCCGACCGGAAAA
  GACTTCGGAAGCATGATGGACTTGGCAAGGGTGTTAAAAGGAAGCTGTTTGCCGAGGATTCTAGCCCTCT
  GAAGAAACAGATTCCTGCATGCTCCGATATGGAGACCCTTTCATCCCCTGTCAAGTTTGGATGTAAGAGT
  AGATCCGCGTCCGCGCTGGATGAAAGCTTTGGGAAGTGTAAACACGAGACCGCTTGTGATTGCTCCGCGA
  TCGAAGAATTGCTGTGTCACGAGTCTCTGCTGGACAGCCCCATGAAGCTTTCCAACGCCCATACTATCTT
  CTCCAGCGACAAGTGGAAGCTGGAATTGGAGAAGATAATCGCCTCTAAGCAAATCTTTCTCGACATGTCA
  GAAAACGTGGAGCTCGTAGCATATGGGGAGACACTGTGCAATCTTAGGATATTCGAGAAAATTAGTTCTC
  CATTTTTGTTTGATGTTCAAAGTGAAGAACGGTCCTACTCTGTGGTCTATGTGCCCCACAATAAAGAACT
  CTGCGGCCAATTCTGCCAACCAGAGAAAACGATGGCCAGAGTGCTTGGCGTTGGGGCTTACGGCAAAGTT
  TTTGATCTTGACAAAGTGGCTATCAAGACAGCTAATGAGGATGAATCAGTGATATCCGCCTTCATCGCTG
  GGGTCATTAGAGCCAAGTCCGGCGCCGACCTGCTGTCCCACGACTGTGTGATCAACAACCTTCTTATATC
  TAACTCAGTGTGTATGGACCATAAAGTTTCTCTGTCTCGGACATATGACGTCGACCTGTATAAATTCGAA
  GATTGGGATGTGCGAAATGTGATGAATTATTACAGCGTGTTCTGCAAATTGGCTGATGCCGTGAGATTTC
  TTAATCTGAAATGTCGCATCAATCACTTCGACATTTCCCCCATGAACATTTTTATTAACCACAAGAAAGA
  GATCATCTTTGATGCGGTACTGGCTGACTATAGCCTTTCCGAAATCCACCCAGAATATAACGGCACTTGC
  GCCATCGCTAAAGAGTACGACCGAAACCTGCAGCTGGTGCCAATCTCAAGAAATAAATTTTGCGACATGT
  TCAATCCTGGCTTCAGACCCCTTGTGGCCAATGCGATGATCCTGGTGAACGTATGTGAAGCTTTTGATGG
  CGAAAATAACCCCCTTCGACATTGTAACTTGGACCTGTGCGCTTTTGCTCAGGTGGTCCTCCTGTGTGTG
  CTCAGAATGACTGATAAGCGCGGCTGTAGAGAGGCCCAACTGTACTACGAAAAGAGACTGTTTGCCCTGG
  CCAATGAAGCATGTAGGCTGAACCCACTCCGGTACCCTTTTGCCTACAGAGATGCCTGTTGTAAGGTGCT
  GGCTGAGCACGTCGTGCTGTTGGGACTCCTGTTCTATAGGGACGTCGTTGATATTTATGAAAAGATATAC
  GACTTCTTGGATGAGAGAGGCGAGTTCGGACTGAGGGATCTGTTCGAGGCGACTTTCCTGAACAATAGTA
  AATTGACCAGAAGACAGCCCATCAGAGGCGGGTTGGCCAGTTTGCAGAGTTCCGAATATGGCGAAAAGCT
  GTTGCACGATCTCCGCGCCTTGTTTCTGATAACCTCCAGCGCTGACCTGGATAAGGACACATCCTCTCTT
  TTTCAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101508 representing NP_050248
  Red=Cloning sites Green=Tags
  
  MDNGVETPQGQKTQPINLPPDRKRLRKHDGLGKGVKRKLFAEDSSPLKKQIPACSDMETLSSPVKFGCKS
  RSASALDESFGKCKHETACDCSAIEELLCHESLLDSPMKLSNAHTIFSSDKWKLELEKIIASKQIFLDMS
  ENVELVAYGETLCNLRIFEKISSPFLFDVQSEERSYSVVYVPHNKELCGQFCQPEKTMARVLGVGAYGKV
  FDLDKVAIKTANEDESVISAFIAGVIRAKSGADLLSHDCVINNLLISNSVCMDHKVSLSRTYDVDLYKFE
  DWDVRNVMNYYSVFCKLADAVRFLNLKCRINHFDISPMNIFINHKKEIIFDAVLADYSLSEIHPEYNGTC
  AIAKEYDRNLQLVPISRNKFCDMFNPGFRPLVANAMILVNVCEAFDGENNPLRHCNLDLCAFAQVVLLCV
  LRMTDKRGCREAQLYYEKRLFALANEACRLNPLRYPFAYRDACCKVLAEHVVLLGLLFYRDVVDIYEKIY
  DFLDERGEFGLRDLFEATFLNNSKLTRRQPIRGGLASLQSSEYGEKLLHDLRALFLITSSADLDKDTSSL
  FQM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1689 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050248
• RefSeq ORF: 1689
• Locus ID: 1497069
• MW: 63.9 kDa