U10 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101453

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp016 [Human herpesvirus 6], codon optimized for human cell expression, NP_050191

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101453 represents NCBI reference of NP_050191 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATTGTGACATACAAGACTGCCTCCGCCCGGAGTCCGACGGTAACGTGGTCCTCTGGATTTGGGA
  GAGCTATTGCCAGCATACAGAAACGGCATCAGGAGAATATCAGAAAGCCCCTGCGCTTTTATAGCGGCCT
  GCTGCACTGTCTGATCAAACAGTACGAGCATTGTCTTGTGCCCCCCAACAAGTCTATACGGTTTGATAAA
  GGCAAGATCGAGGTGGCTGCATTGATTCTTGACTTGGGACATCAGGTGCTGGGCCGACAGATACATGTGC
  GGCAGAGGATTTATTCTTGGACCAGTATCACCCTGCCAAAGCTCTTTACCCCCCGGGAGCTCTACTTCCT
  TGTGGCGAGCCCGGAGGATGAAGACATCGTGTTCAACCCCACAATAACTAAAGGAGGGTGGATCAGCGGC
  AGTTTTAGTTATCCGGTGGAGTATAGAAGCAATTTCTCCTTGACTGGCATGTCCGCCAACGTGCTCATGG
  TGCCCTTCGTGCCTTACCGCTACCCGTTGAACTATGCCCGCTTCATCAGTTCCATCGACCTGATGATCCT
  GAATGAACAGTTTCCCGAACATGAGTGTGGCGATATTCAGATCTTGAAACAGAGAAACTACCTCTACCTG
  GGAGTTATCAAGAACCTTACCTGGAAAAAGTCCGTCACTGGGACAGGGCAGACAGCCCCACACCGAATAC
  TGAAGGCCTCTTTTATCGGGAGCTGGCCCGATACCTCCCTGCCTGACCGCGTGGCTCTCCGATTTTTTAA
  CAATACGAGGTTCACTATCCACTGCCACGAGTTCGCGATAAACATCGAGAACTTGGGCCTTGTGAAGAAC
  AAGGAGAAGGTGTTTGGAACACTTGCCACAGTCTGCTGTGAGCAAATCCCTTCCCTGCTGACGACTGAGA
  ATCTGCCCAGATATCTGATAGTTCAGTTCGAAGTCGTCACACAGATTGAGGATCCCGAGCCGCTTCTTTT
  CTCCAGTAATCCCAAGCTCTACTTCACCGGTGACGTGCTTAACGCTACTATGCAGCTGCAACACAACCCC
  AATTACTACGATCTCCTCGTGCACGCACCTTACGACATTCATTTCTATCCCTCTCGGTGCCACATTGTAA
  TTCTGCCTATCCGGTATTTTACCAGAGGCGACAAACAGATTCTCATTAGCGGCTACCAGAACGAGGGGTT
  CTTTGAGACCCAGGTGATGCTTTGGGCACCAGGGACACCACTTCACATCACCCTCAGGTCATTTTCTCCC
  AATCTGATCCTCCCTCAGTCCACGCCCATCGCCACTCTGTTCTACGTGGAGAGAATGACATCCCAGAATA
  CAGAGCAAAAGGACGTAATAGCCAAGCTGTCCGAGAATGGTCACTTTATAGGAAACCTGAAACTGCCCCG
  CGAGAACTTCCTGCATCACGACGCCATAACAGACCTCAGCCTCGCCGCCATCCCAAAGGATTCCGCCACC
  CCAGGCCCCGGCACCGTGAGTAGTTCTGTGTCTCCGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101453 representing NP_050191
  Red=Cloning sites Green=Tags
  
  MEIVTYKTASARSPTVTWSSGFGRAIASIQKRHQENIRKPLRFYSGLLHCLIKQYEHCLVPPNKSIRFDK
  GKIEVAALILDLGHQVLGRQIHVRQRIYSWTSITLPKLFTPRELYFLVASPEDEDIVFNPTITKGGWISG
  SFSYPVEYRSNFSLTGMSANVLMVPFVPYRYPLNYARFISSIDLMILNEQFPEHECGDIQILKQRNYLYL
  GVIKNLTWKKSVTGTGQTAPHRILKASFIGSWPDTSLPDRVALRFFNNTRFTIHCHEFAINIENLGLVKN
  KEKVFGTLATVCCEQIPSLLTTENLPRYLIVQFEVVTQIEDPEPLLFSSNPKLYFTGDVLNATMQLQHNP
  NYYDLLVHAPYDIHFYPSRCHIVILPIRYFTRGDKQILISGYQNEGFFETQVMLWAPGTPLHITLRSFSP
  NLILPQSTPIATLFYVERMTSQNTEQKDVIAKLSENGHFIGNLKLPRENFLHHDAITDLSLAAIPKDSAT
  PGPGTVSSSVSPS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050191
• RefSeq ORF: 1509
• Locus ID: 1497010
• MW: 57.2 kDa