U54 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101495

Myc-DDK-tagged ORF clone of viral ORF for virion transactivator [Human herpesvirus 6], codon optimized for human cell expression, NP_050235

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101495 represents NCBI reference of NP_050235 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCAGCAACCCTGCAGTGGTCCAGCTATGTGCTCCAGCTGAGACTGACTACAGCCGCCATACTTA
  AACCTGGAGAGTTGCGGTATTTCAAATGCGGCCTGGGAATTTGTCCGCCCTCTAGCTCCGTGGTATGCAC
  TTGTAGAGACGAGAGCTCATTCGCCGCCAGCCCATTCACCTACATAGACCCTAAGGATTACGGAAACATC
  CCCTTTGCTGTCCATAACATTTCTGACCTTGACCTGGACCTGGGAAGACTGCCTATCGTCCTGAATATAT
  TCGCACTGCCCTATGCCAACGTCACTGTGTCCAATCTTCCCGTTCAGCGCATCGAGGCATATGATCGGCA
  TGCCATTCCTCACGGTCAGTGTGAAGCTCAGTTTATCCTGTATGGACCTCTCACTAGAATTAAGATTTAT
  GCGGCCGATATCAGATGGACCGAAAAGACCCCTGAAGAACCTACCCGGTATCTCTTCAATGCTGACATAT
  GGATTAACCTGCAGAATACTCCTCTCGACCAGATTTTCAATTCTGCCAAGATCGAGTTTATTTCTCACAG
  ACATGTGTCAATAAGCCGCATCCTTTTGTGTGGCAATCTCCTCATATTGAAGACCGTTTACGAGAACGAC
  TACCTCTTGGACGACACCTTTTTCCCAGAGAAACTGAACATTCAGCTGAGCCTGACTCCTCACACAGCAG
  ACATTACCATGTCCCAGAATCAGGAAACCTTGCTTAAGTGTAACGTGGGATCAATCACCGTCGGAGCTAC
  TAAAAACATCGTGTCCAACCTCATTACACCCTTTCACTGTTCTTTCCATACCATATTCGAGAGCAATAGC
  AACTTCACCGGGTTCTTCATTCCAAAACTGCTGCATGGAATTAGCATTACGACTTGCATATGGGACGAAA
  CACGGCCCCTCTATATTACCATGAAGGCCCAGAAAAAAAACTGTAGAATTAACTATTCCCAAGAGCTGGG
  GAAAGTTTTCTTCTTCCCCAAGCAGATCCTTCCTCCCGGAAACAATATCGAGTTCAGTTGGACAGAGATG
  TCCAACATATATGTTAACATCGAAAGCCCTAATGGACCCGTAAAAGACATTGTCGCACTGGCGAGTCAGC
  CAGTCAGTCGCGCCCCTAGTCTCACTACCGTGGCCCATGTGACGCCCGAGATCTCAATGGGAATCTTCTT
  GAAGTCCCTCCGGATCGCTTTTGACAAAGAGCACCTCGTGCCCCTGCATTTTTTCCTCAAACCTGGCGAA
  TCAACGCGCATGGAATTCATGCCCCCAGAGACACCACAATCTCTGACCATTTTGGAAGGGGACGTCGGAA
  TTCGCTTCATCCCTTGCCATAATAATTACTCCCACAGGAGCAGCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101495 representing NP_050235
  Red=Cloning sites Green=Tags
  
  MQPATLQWSSYVLQLRLTTAAILKPGELRYFKCGLGICPPSSSVVCTCRDESSFAASPFTYIDPKDYGNI
  PFAVHNISDLDLDLGRLPIVLNIFALPYANVTVSNLPVQRIEAYDRHAIPHGQCEAQFILYGPLTRIKIY
  AADIRWTEKTPEEPTRYLFNADIWINLQNTPLDQIFNSAKIEFISHRHVSISRILLCGNLLILKTVYEND
  YLLDDTFFPEKLNIQLSLTPHTADITMSQNQETLLKCNVGSITVGATKNIVSNLITPFHCSFHTIFESNS
  NFTGFFIPKLLHGISITTCIWDETRPLYITMKAQKKNCRINYSQELGKVFFFPKQILPPGNNIEFSWTEM
  SNIYVNIESPNGPVKDIVALASQPVSRAPSLTTVAHVTPEISMGIFLKSLRIAFDKEHLVPLHFFLKPGE
  STRMEFMPPETPQSLTILEGDVGIRFIPCHNNYSHRSSP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050235
• RefSeq ORF: 1377
• Locus ID: 1497056
• MW: 51.8 kDa