U56 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101497

Myc-DDK-tagged ORF clone of viral ORF for capsid protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050237

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101497 represents NCBI reference of NP_050237 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCGTGTACTGTACCTTTGATCATAAGCTGTCCCTGAGTGACATCTCCACGCTTTGTAAGCTTA
  TGAACATCGTCATCCCGATCCCCGCCCACCACCACTTGATCGGGTCCGGCAATTTGGGTCTCTATCCAAT
  CGTCTCCTCCAATAAGGATTATGTGCATATTCGGAATGTTCTTCGCACAATGGTTGTCACAATCCTCCAG
  AAGGTGGAGGGAAACCAACTCGTACTGCGAAAGCCCATGACCGGGCAGCAGTATGCTATCAAAAATACCG
  GCCCATTCCCCTGGGAGAAGGGTGACACACTCACCCTGATCCCACCCCTGTCAACCCATAGCGAGGAAAA
  GCTCCTGAAGCTTGGCGACTGGGAACTGACAGTGCCATTGGTGGTGCCCACAGCTATTGCCGCCGAGATA
  AATATCCGCCTGCTTTGTATCGGCCTGATTGCGGTCCATCGGGAGTATAATGAAATGCAAACTATTATTG
  ATGAATTGTGCTCCATTCAATACAGAGATGTACTGATCAAACTGCCCGACATCGTTAATGACAAACAGAG
  TATGTACTCTATGAAGACGGCATGCATCTCACTGAGTATGATCACCGCCATGGCGCCAGACATTGTGAGA
  ACATACATCGACAGGTTGACATTGGAGGACCACTCTATGTTGCTCATTAAATGCCAGGAGCTGCTTTCCA
  AGAGGACCACCCTTTCCACCCAGAGGTGCGGTCAACTCCATGCAACTGATATCAAGGATGAACTCAAAAA
  AATCAAGAGCGTGCTCACGATGATAGATCAAATCAATAGCCTCACCAACGAAAAAACCTACTTTGTCGTC
  TGTGATGTCTCTGCCGACAATCGAATGGCAACATGCATCTACAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101497 representing NP_050237
  Red=Cloning sites Green=Tags
  
  METVYCTFDHKLSLSDISTLCKLMNIVIPIPAHHHLIGSGNLGLYPIVSSNKDYVHIRNVLRTMVVTILQ
  KVEGNQLVLRKPMTGQQYAIKNTGPFPWEKGDTLTLIPPLSTHSEEKLLKLGDWELTVPLVVPTAIAAEI
  NIRLLCIGLIAVHREYNEMQTIIDELCSIQYRDVLIKLPDIVNDKQSMYSMKTACISLSMITAMAPDIVR
  TYIDRLTLEDHSMLLIKCQELLSKRTTLSTQRCGQLHATDIKDELKKIKSVLTMIDQINSLTNEKTYFVV
  CDVSADNRMATCIYKN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050237
• RefSeq ORF: 888
• Locus ID: 1497058
• MW: 33.5 kDa