U81 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101520

Myc-DDK-tagged ORF clone of viral ORF for Uracyl-DNA glycosylase [Human herpesvirus 6], codon optimized for human cell expression, NP_050260

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U81
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101520 represents NCBI reference of NP_050260 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGCTGCAGTGGATGTTGGACCACGTGCAAGACGAAGAGAAAAACTACGAAAACCTTTCAATCG
  ACGACCAGCACAGCCTTTTTGGCATCAACAGAGACTGGCTGTCCTTCCTTCAACTCAGCAAACTCGAAAT
  CACTCACTTGAAACATGTGTATAAACTTGTGGACAATGACCGCGTGCATCTGACAGTTCACCCGTCAAGC
  GATAATGTCCATGCATGGAGCTTTCTGTGTAAGCCGACCGACGTGAAGGTGGTTATTCTCGGCCAGGACC
  CTTACCCAGACGGCCGGGGCTGCGGACTGGCTTTCGGAACTGTTAAAGAGTGCAGCATTCCTGAGTCTCT
  GAAGAACATTTTTAAAGAACTCGAGCGGAGTATCCCAAATTTCTCTCCACCCGACAATGGCTGCCTGAAT
  TCCTGGTGTAGGGAAGGAGTTCTCCTGCTGAATAGCATCTTCACTGTCGTGCATGGCCTGCCCATGAGTC
  ACGAGGCCTTCGGGTGGCAAACTCTGAGCTACAAAATAATTAGTCGACTTTCCGAGCAGATGAACTCTCT
  GGTCTTTCTGCTGTGGGGGAAACACGCACGCAAACTTTCCTACCTGATCGACGCCCAGAAACACCTGGTC
  CTCGAGTCAGCCCATCCAAGCCCTAAGGTCAAATCAGCCCGAATGCCCTTTATTGGGTGTAACCACTTTG
  TGCGCACCAATCTTTTTCTGACTGAGCACGGTAAAGATCCCATAAATTGGAACATTCTGAACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101520 representing NP_050260
  Red=Cloning sites Green=Tags
  
  MALLQWMLDHVQDEEKNYENLSIDDQHSLFGINRDWLSFLQLSKLEITHLKHVYKLVDNDRVHLTVHPSS
  DNVHAWSFLCKPTDVKVVILGQDPYPDGRGCGLAFGTVKECSIPESLKNIFKELERSIPNFSPPDNGCLN
  SWCREGVLLLNSIFTVVHGLPMSHEAFGWQTLSYKIISRLSEQMNSLVFLLWGKHARKLSYLIDAQKHLV
  LESAHPSPKVKSARMPFIGCNHFVRTNLFLTEHGKDPINWNILNE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050260
• RefSeq ORF: 765
• Locus ID: 1497081
• MW: 29.2 kDa