U39 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101480

Myc-DDK-tagged ORF clone of viral ORF for Glycoprotein B [Human herpesvirus 6], codon optimized for human cell expression, NP_050220

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101480 represents NCBI reference of NP_050220 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAAATGCGGGTGTTGTTCCTGGCAGTGTTTCTGATGAACAGTGTTCTGATGATTTATTGTGATT
  CCGATGATTATATACGCGCAGGATACAACCACAAATACCCGTTCCGGATCTGCTCTATTGCCAAAGGGAC
  AGATCTCATGCGCTTCGACAGGGACATCTCCTGCTCCCCCTATAAGAGTAATGCTAAAATGTCCGAAGGC
  TTTTTTATCATATATAAAACAAATATCGAAACGTATACATTCCCAGTCAGGACCTATAAGAACGAGCTGA
  CCTTTCCTACTTCTTACAGAGACGTGGGGGTGGTGTACTTTCTGGATCGAACGGTAATGGGGTTGGCAAT
  GCCTGTTTATGAGGCAAATCTTGTGAACAGCAGAGCCCAGTGTTACTCCGCCGTGGCCATCAAAAGGCCC
  GATGGCACCGTGTTCTCTGCCTATCACGAAGACAACAATAAAAACGAGACACTGGAGTTGTTCCCGCTCA
  ATTTTAAGTCTGTGACCAATAAAAGGTTCATTACCACTAAAGAACCATACTTTGCTCGAGGCCCACTTTG
  GCTGTATAGCACTTCAACTTCCCTGAACTGTATCGTCACTGAGGCCACTGCAAAGGCTAAGTATCCCTTT
  AGCTACTTTGCCCTGACGACGGGCGAAATAGTGGAGGGGAGCCCTTTCTTCGATGGTAGCAACGGTAAAC
  ACTTTGCCGAACCCCTCGAAAAACTGACCATTTTGGAAAATTATACAATGATTGAAGATCTGATGAACGG
  AATGAACGGAGCTACAACCCTGGTAAGAAAAATCGCGTTCTTGGAAAAAGGAGACACATTGTTCAGTTGG
  GAGATCAAGGAAGAAAACGAGTCCGTGTGCATGCTGAAACACTGGACTACTGTCACACACGGCCTGCGCG
  CCGAAACAGATGAGACTTATCATTTCATTTCCAAGGAGCTGACCGCAGCATTTGTGGCTTCTAAAGAGAG
  CCTGAACCTCACCGATCCAAAACAGACGTGTATAAAAAATGAATTTGAGAAGATCATCACAGATGTGTAC
  ATGAGTGACTATAATGACGCTTACTCAATGAATGGCTCTTACCAGATTTTCAAAACCACAGGGGATTTGA
  TATTGATTTGGCAGCCACTCGTACAGAAAAGTCTGATGGTACTGGAACAGGGCTCAGTTAATTTGAGGAG
  ACGCAGAGATCTGGTGGACGTGAAGAGTAGGCACGATATTCTGTACGTGCAGCTTCAGTATCTGTATGAT
  ACTTTGAAGGACTACATTAATGATGCGCTGGGAAACCTGGCCGAGAGCTGGTGCCTGGATCAGAAGCGAA
  CAATAACCATGCTGCACGAGCTCAGTAAAATTTCTCCGAGCTCCATTGTCTCCGAGGTCTATGGAAGGCC
  TATCTCCGCCCAACTGCATGGAGACGTTCTCGCTATTAGTAAGTGTATCGAGGTTAACCAGTCATCAGTC
  CAGCTGTATAAATCAATGAGGGTGGTGGACGCTAAGGGAGTCAGAAGCGAAACTATGTGCTATAACAGAC
  CTTTGGTGACATTTTCTTTCGTGAATAGCACACCAGAAGTGGTGCTGGGACAATTGGGTCTCGACAACGA
  GATTCTCCTGGGTGATCACCGCACTGAGGAGTGTGAGATCCCCTCTACTAAGATCTTCCTCTCCGGAAAC
  CACGCCCACGTTTACACAGACTATACTCACACGAATAGCACCCCCATTGAGGACATTGAGGTTTTGGATG
  CTTTTATAAGACTGAAAATCGATCCACTTGAAAATGCCGACTTCAAACTGCTCGACCTGTATAGCCCGGA
  CGAACTCAGTAGAGCTAACGTCTTCGATCTCGAGAATATCCTTCGGGAGTACAACAGCTATAAGAGTGCT
  CTGTATACGATAGAGGCGAAAATAGCTACAAATACCCCATCCTACGTTAACGGCATCAACTCCTTCCTCC
  AGGGGCTCGGAGCTATAGGCACTGGGCTGGGCTCCGTTATCAGCGTCACTGCAGGAGCGCTGGGTGATAT
  CGTGGGAGGAGTGGTGAGTTTTCTGAAAAACCCTTTTGGTGGAGGTCTTATGCTGATCCTCGCCATAGTT
  GTTGTGGTGATAATAATCGTGGTGTTTGTTCGACAGAAGCATGTATTGAGTAAGCCAATTGATATGATGT
  TCCCGTATGCCACCAATCCTGTTACGACGGTTTCCTCTGTCACAGGAACGACGGTGGTGAAGACACCTAG
  CGTGAAGGACGCAGACGGCGGCACCTCCGTGGCAGTGAGCGAGAAGGAGGAGGGGATGGCAGATGTGTCA
  GGACAAATCTCCGGGGACGAATACTCACAGGAGGACGCGCTCAAGATGTTGAAGGCCATCAAATCACTGG
  ACGAGAGCTATAGAAGGAAACCCAGCTCCTCAGAAAGTCACGCCTCTAAACCCTCACTGATTGACAGGAT
  CCGGTACCGAGGATACAAATCTGTTAACGTGGAAGAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101480 representing NP_050220
  Red=Cloning sites Green=Tags
  
  MSKMRVLFLAVFLMNSVLMIYCDSDDYIRAGYNHKYPFRICSIAKGTDLMRFDRDISCSPYKSNAKMSEG
  FFIIYKTNIETYTFPVRTYKNELTFPTSYRDVGVVYFLDRTVMGLAMPVYEANLVNSRAQCYSAVAIKRP
  DGTVFSAYHEDNNKNETLELFPLNFKSVTNKRFITTKEPYFARGPLWLYSTSTSLNCIVTEATAKAKYPF
  SYFALTTGEIVEGSPFFDGSNGKHFAEPLEKLTILENYTMIEDLMNGMNGATTLVRKIAFLEKGDTLFSW
  EIKEENESVCMLKHWTTVTHGLRAETDETYHFISKELTAAFVASKESLNLTDPKQTCIKNEFEKIITDVY
  MSDYNDAYSMNGSYQIFKTTGDLILIWQPLVQKSLMVLEQGSVNLRRRRDLVDVKSRHDILYVQLQYLYD
  TLKDYINDALGNLAESWCLDQKRTITMLHELSKISPSSIVSEVYGRPISAQLHGDVLAISKCIEVNQSSV
  QLYKSMRVVDAKGVRSETMCYNRPLVTFSFVNSTPEVVLGQLGLDNEILLGDHRTEECEIPSTKIFLSGN
  HAHVYTDYTHTNSTPIEDIEVLDAFIRLKIDPLENADFKLLDLYSPDELSRANVFDLENILREYNSYKSA
  LYTIEAKIATNTPSYVNGINSFLQGLGAIGTGLGSVISVTAGALGDIVGGVVSFLKNPFGGGLMLILAIV
  VVVIIIVVFVRQKHVLSKPIDMMFPYATNPVTTVSSVTGTTVVKTPSVKDADGGTSVAVSEKEEGMADVS
  GQISGDEYSQEDALKMLKAIKSLDESYRRKPSSSESHASKPSLIDRIRYRGYKSVNVEEA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2490 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050220
• RefSeq ORF: 2490
• Locus ID: 1497041
• MW: 93.1 kDa