U57 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101498

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050238

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101498 represents NCBI reference of NP_050238 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATTGGCAAGCCACCGAAATCCTCCCAAAAATCGAAGCACCCCTCAATATTTTTAACGATATTA
  AGACCTACACTGCTGAGCAGCTGTTCGACAATCTTCGCATATATTTCGGCGACGATCCCAGCCGGTATAA
  CATTAGCTTCGAAGCACTTCTTGGCATCTACTGCAACAAGATCGAGTGGATAAACTTTTTCACAACCCCC
  ATCGCAGTCGCCGCAAACGTGATCAGATTTAACGACGTCTCACGAATGACCCTGGGAAAGGTTTTGTTCT
  TCATTCAGCTCCCGCGCGTGGCCACCGGTAATGATGTAACAGCACCCAAGGAAACCACCATTATGGTAGC
  CAAGCACAGTGAGAAGCACCCTATCAATATATCTTTTGATTTGAGCGCCGCGTGTCTTGAACATTTGGAG
  AATACTTTTAAAAATACAGTGATCGACCAAATCCTGAACATCAACGCCCTGCATACAGTGCTTAGGTCTC
  TGAAAAATTCCGCTGACAGTCTCGAGAGAGGACTCATACACGCATTCATGCAGACACTGCTGCGGAAGAG
  CCCCCCGCAATTCATAGTATTGACCATGAACGAGAACAAAGTTCACAATAAGCAAGCCCTCTCCAGGGTT
  CAGCGGTCCAATATGTTCCAGAGCCTGAAGAATCGGCTCCTGACTAGTCTGTTTTTCTTGAACAGAAATA
  ACAACTCTTCTTACATTTATAGAATTTTGAACGATATGATGGAGTCAGTCACTGAGTCCATTTTGAACGA
  TACTAATAACTACACCTCCAAAGAGAATATTCCTCTTGATGGCGTGCTGCTCGGACCAATTGGCAGTATC
  CAGAAGCTCACCAACATACTCTCCCAGTATATTTCCACCCAGGTGGTCTCTGCCCCAATTTCTTATGGGC
  ACTTTATAATGGGCAAAGAGAACGCAGTCACTGCTATCGCTTACCGCGCTATTATGGCCGATTTCACCCA
  GTTCACCGTCAACGCCGGAACAGAGCAGCAGGATACCAACAATAAAAGTGAGATCTTCGATAAAAGCCGG
  GCATACGCTGACTTGAAGCTTAATACATTGAAGCTGGGGGACAAACTGGTGGCATTTGATCACCTTCACA
  AGGTGTATAAAAATACTGACGTTAATGACCCTCTGGAGCAGTCCCTGCAGCTGACTTTTTTCTTCCCCCT
  CGGGATTTATATTCCTACGGAAACTGGCTTTAGTACAATGGAAACTAGAGTGAAGCTCAACGACACAATG
  GAAAACAATCTTCCTACCAGCGTGTTCTTCCACAACAAAGATCAGGTGGTGCAGAGAATTGATTTCGCTG
  ACATACTTCCGTCCGTATGCCATCCAATTGTTCACGACTCCACCATTGTGGAACGACTGATGAAAAACGA
  GCCACTGCCTACTGGACATCGGTTTAGTCAGCTCTGTCAGCTGAAGATCACTAGGGAGAATCCAACGCGG
  ATATTGCAGACCCTCTATAACCTGTACGAATCAAGACAAGAGGTCCCGAAGAATACAAATGTCTTGAAGA
  ACGAGCTGAACGTGGAGGATTTCTACAAACCCGACAACCCAACTCTCCCCACCGAACGCCATCCGTTTTT
  TGATCTCACCTATATCCAGAAGAACCGGGCCACCGAAGTGCTCTGTACCCCGCGCATAATGATCGGTAAC
  ATGCCACTTCCTCTGGCTCCGATTTCTTTTCATGAGGCCCGAACCAATCAGATGCTGGAGCATGCTAAAA
  CTAACAGCCATAATTATGATTTTACACTTAAAATCGTTACAGAGTCTCTGACTTCCGGGTCATATCCGGA
  ATTGGCATACGTGATCGAAATTCTGGTCCATGGCAACAAACACGCCTTCATGATCTTGAAACAGGTGATT
  AGCCAGTGCATATCTTATTGGTTCAACATGAAGCATATCCTTCTGTTCTGCAACAGTTTCGAGATGATAA
  TGCTTATTTCCAATCACATGGGGGACGAACTGATACCAGGGGCCGCCTTCGCCCACTACAGGAATCTGGT
  GAGTCTTATTAGACTGGTGAAACGCACCATCAGTATAAGTAACATCAATGAGCAGCTTTGTGGTGAGCCT
  TTGGTAAATTTCGCCAACGCCCTCTTTGACGGCCGCCTCTTCTGTCCCTTCGTCCATACAATGCCAAGAA
  ACGACACCAATGCCAAAATTACCGCGGACGATACCCCTCTGACGCAGAATACCGTCAGGGTCCGGAACTA
  CGAGATCTCAGACGTGCAGCGGATGAACTTGATAGACAGCTCCGTGGTGTTTACAGACAATGACAGGCCT
  TCTAATGAGAACACTATACTCAGCAAAATTTTCTATTTCTGTGTCCTGCCGGCTCTGAGCAATAATAAGG
  CCTGCGGGGCCGGCGTGAATGTGAAGGAGCTTGTGCTGGATCTGTTCTACACCGAACCCTTTATATGTCC
  TGACGACTGTTTCCAGGAAAATCCTATCAGTAGCGACGTTCTCATGAGCCTCATCCGCGAAGCGATGGGT
  CCGGGCTACACGGTCGCGAATACATCCTCCATTGCTAAACAACTGTTCAAAAGCCTGATCTATATCAATG
  AAAACACAAAAATTCTCGAAGTCGAAGTCTCTCTCGACCCGGCTCAAAGACACGGCAACAGCGTGCACTT
  TCAGTCACTGCAGCACATCCTGTATAACGGGCTGTGTCTCATCTCTCCTATCACCACACTCAGAAGATAC
  TATCAGCCCATCCCCTTCCACCGCTTTTTCTCCGATCCAGGAATCTGTGGTACGATGAATGCCGACATTC
  AGGTGTTCCTCAACACCTTTCCACACTACCAGAGGAACGACGGTGGCTTTCCACTGCCACCTCCACTGGC
  ACTTGAGTTTTATAATTGGCAGAGGACACCCTTTTCTGTGTACAGTGCCTTCTGTCCGAACAGCCTGCTT
  TCTATTATGACCTTGGCAGCTATGCACTCAAAACTGAGCCCTGTCGCCATAGCTATTCAGTCTAAATCAA
  AAATTCACCCCGGGTTCGCAGCTACCCTCGTGCGGACTGACAATTTTGACGTAGAATGCCTCCTGTACAG
  CAGCAGGGCAGCAACGTCTATAATCCTCGACGATCCAACCGTGACCGCAGAAGCCAAGGATATCGTAACA
  ACTTATAATTTCACCCAGCATCTGTCATTCGTTGATATGGGGCTCGGGTTCAGTTCAACCACAGCTACTG
  CTAACCTTAAACGCATCAAGTCCGATATGGGGAGCAAGATCCAGAACCTCTTCTCCGCATTCCCCATCCA
  TGCCTTCACCAATACGGACATCAATACCTGGATACGACACCATGTGGGCATCGAAAAACCAAACCCCAGT
  GAAGGGGAAGCACTTAATATCATCACGTTTGGGGGGATTAATAAGAATCCTCCATCTATCCTGCTGCATG
  GCCAACAGGCCATTTGCGAAGTAATCCTTACACCTGTTACCACCAATATCAACTTCTTCAAGCTGCCGCA
  CAACCCACGCGGCAGAGAGTCTTGTATGATGGGTACCGATCCTCACAACGAGGAGGCCGCCCGCAAGGCC
  CTGTATGATCATACCCAGACCGACAGCGACACCTTCGCGGCCACGACCAATCCATGGGCCTCTCTGCCCG
  GGTCTCTGGGAGACATTTTGTATAACACGGCTCACCGAGAGCAGCTGTGTTACAACCCGAAAACGTACTC
  CCCCAACGCCCAATTCTTTACGGAGTCTGATATTCTGAAGACCAATAAAATGATGTACAAAGTAATAAAC
  GAATACTGCATGAAGTCCAACAGTTGTCTGAACTCCGACTCCGAGATCCAGTACTCATGCAGCGAGGGCA
  CAGACTCCTTTGTCTCCCGGCCCTGTCAATTTCTGCAAAATGCCCTCCCACTGCACTGCTCAAGCAATCA
  AGCCCTGCTCGAATCTAGGTCAAAAACCGGCAACACACAAATTAGCGAGACACACTACTGTAACTACGCC
  ATCGGCGAAACGATCCCCCTTCAGCTGATCATAGAATCATCAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101498 representing NP_050238
  Red=Cloning sites Green=Tags
  
  MENWQATEILPKIEAPLNIFNDIKTYTAEQLFDNLRIYFGDDPSRYNISFEALLGIYCNKIEWINFFTTP
  IAVAANVIRFNDVSRMTLGKVLFFIQLPRVATGNDVTAPKETTIMVAKHSEKHPINISFDLSAACLEHLE
  NTFKNTVIDQILNINALHTVLRSLKNSADSLERGLIHAFMQTLLRKSPPQFIVLTMNENKVHNKQALSRV
  QRSNMFQSLKNRLLTSLFFLNRNNNSSYIYRILNDMMESVTESILNDTNNYTSKENIPLDGVLLGPIGSI
  QKLTNILSQYISTQVVSAPISYGHFIMGKENAVTAIAYRAIMADFTQFTVNAGTEQQDTNNKSEIFDKSR
  AYADLKLNTLKLGDKLVAFDHLHKVYKNTDVNDPLEQSLQLTFFFPLGIYIPTETGFSTMETRVKLNDTM
  ENNLPTSVFFHNKDQVVQRIDFADILPSVCHPIVHDSTIVERLMKNEPLPTGHRFSQLCQLKITRENPTR
  ILQTLYNLYESRQEVPKNTNVLKNELNVEDFYKPDNPTLPTERHPFFDLTYIQKNRATEVLCTPRIMIGN
  MPLPLAPISFHEARTNQMLEHAKTNSHNYDFTLKIVTESLTSGSYPELAYVIEILVHGNKHAFMILKQVI
  SQCISYWFNMKHILLFCNSFEMIMLISNHMGDELIPGAAFAHYRNLVSLIRLVKRTISISNINEQLCGEP
  LVNFANALFDGRLFCPFVHTMPRNDTNAKITADDTPLTQNTVRVRNYEISDVQRMNLIDSSVVFTDNDRP
  SNENTILSKIFYFCVLPALSNNKACGAGVNVKELVLDLFYTEPFICPDDCFQENPISSDVLMSLIREAMG
  PGYTVANTSSIAKQLFKSLIYINENTKILEVEVSLDPAQRHGNSVHFQSLQHILYNGLCLISPITTLRRY
  YQPIPFHRFFSDPGICGTMNADIQVFLNTFPHYQRNDGGFPLPPPLALEFYNWQRTPFSVYSAFCPNSLL
  SIMTLAAMHSKLSPVAIAIQSKSKIHPGFAATLVRTDNFDVECLLYSSRAATSIILDDPTVTAEAKDIVT
  TYNFTQHLSFVDMGLGFSSTTATANLKRIKSDMGSKIQNLFSAFPIHAFTNTDINTWIRHHVGIEKPNPS
  EGEALNIITFGGINKNPPSILLHGQQAICEVILTPVTTNINFFKLPHNPRGRESCMMGTDPHNEEAARKA
  LYDHTQTDSDTFAATTNPWASLPGSLGDILYNTAHREQLCYNPKTYSPNAQFFTESDILKTNKMMYKVIN
  EYCMKSNSCLNSDSEIQYSCSEGTDSFVSRPCQFLQNALPLHCSSNQALLESRSKTGNTQISETHYCNYA
  IGETIPLQLIIESSI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 4035 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050238
• RefSeq ORF: 4035
• Locus ID: 1497059
• MW: 152.1 kDa