U84 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101523

Myc-DDK-tagged ORF clone of viral ORF for putative glycoprotein [Human herpesvirus 6], codon optimized for human cell expression, NP_050263

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101523 represents NCBI reference of NP_050263 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACAGAGGTAATAAGAACACTTCTAAGACCCGACCCACCGTGAAATCCCGACGAACTCGGAAAA
  CCACCAAGCCATCTACTGAGAGGAAGAAAAATGTGACCACAAAGACCACGATCAGGAACAATCGCCTGCA
  GAACAATCTGTCCAGCCAGACCGTCCAGACCGACAAGCCCGTTGATTCAGCAACCGCTAGAGCCTTCCCG
  CAGCATACAAGCCCCGACATCAAATTTAATAAAGAAAAATACCGAAATAACGCTATCTACGCCCACTCCA
  AGCTGAGGAAAATCTCCAACGCTGTTTTTCACCAGGATAAGGAAATTAACCGGTTTTCAGCGTTCACCGA
  CAAACCTCTGGCTGACCTTTCTTTTAATATGCCTGTCAAGACCTCTACAACTCAGAAGAAAGAGATCGTA
  AAATCAACTGAGTGTCAACGCCAGGACCCGAACGCCGTAAATGCTTTCAAGATCTTTCAGAGTTATGAGA
  CCAGCCCTCCTTATTCCCCTTGCAGTCCCAAAAACTTTATGAGCGAAATATATAGGCTGTTTCGCTGCTA
  TAATGCCAGCATCGTTCAGATTCAGGTTTACAGTCGGAACAAGATTCTCATTAACGCAATGCAGGAGAAA
  TTGATGAGCATAGGCAATATGGTAATAAACGTGGGCGAACAGATTATTAGCGAGTCTGTTCATCATGAGC
  CAGTAGTGGCTATCGCCATCAACCAGTTTTTTAGAGGCACCGTCCCGCATACAGATGCTTTGAGAAAGAA
  CGCGGTCGCCCCCATTCCAGTCAATGCGAGCAAAAAGAAGCTCGTGGGAATCTGCAGCCTGATGATCAAT
  GCGCCCACTGAGAAAGATCTGCTCTGCGCTGCCCATGTATGCATCAACTTCGCAATCTGTCACCCGAATG
  AAATAACCCTTAACATTGCCAAACTCACCCAGCCTATGTTGCTCCGCGAGCATATCACCATCTACAACAG
  CTTCAACAAGTACATCTATTGGGACGACTATGGCAAACTCCTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101523 representing NP_050263
  Red=Cloning sites Green=Tags
  
  MKDRGNKNTSKTRPTVKSRRTRKTTKPSTERKKNVTTKTTIRNNRLQNNLSSQTVQTDKPVDSATARAFP
  QHTSPDIKFNKEKYRNNAIYAHSKLRKISNAVFHQDKEINRFSAFTDKPLADLSFNMPVKTSTTQKKEIV
  KSTECQRQDPNAVNAFKIFQSYETSPPYSPCSPKNFMSEIYRLFRCYNASIVQIQVYSRNKILINAMQEK
  LMSIGNMVINVGEQIISESVHHEPVVAIAINQFFRGTVPHTDALRKNAVAPIPVNASKKKLVGICSLMIN
  APTEKDLLCAAHVCINFAICHPNEITLNIAKLTQPMLLREHITIYNSFNKYIYWDDYGKLLN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050263
• RefSeq ORF: 1026
• Locus ID: 1497084
• MW: 39.0 kDa