U100 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101531

Myc-DDK-tagged ORF clone of viral ORF for spliced envelope glycoprotein [Human herpesvirus 6], codon optimized for human cell expression, NP_050271

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101531 represents NCBI reference of NP_050271 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCGCCGAGACGGAGCGCTCCAATTCTTGTGTGCGCGATCTCTATGGCCACAGCCCTGAGCAATG
  CGACCGTTCACCGAGACGCTGGTACTGTAGAGAGCACCCCTCCGCCTGATGACGAGGATAACTATACAGC
  TAAATACTACGATGACTCTATTTACTTTAACATTTACGATGGAACAAACCCCACCCCAAGGCGGCGGACG
  TTGCCGGAGATCATAAGTAAGTTTAGTACTTCCGAGATGTCCCGACTGGGAGGCCTCAAAGCTTTTGTGC
  CCGTAGATTATACCCCCACTACAACACTGGAGGATATAGAGGACCTGCTCAATTATGCTATCTGTGACGA
  TAATAGTTGTGGGTGTCTTATTGAAACAGAGGCAAGGATCATGTTCGGGGACATCATTATTTGTGTACCA
  CTTTCAGCAGAATCCCGGGGGGTGCGCAACCTTAAATCACGCATTATGCCTATGGGTCTCTCTCAAATCC
  TGTCCAGCGGGCTCGGACTGCATTTTAGCCTCCTCTATGGAGCCTTCGGCAGCAACTACAACAGCTTGGC
  TTACATGGAGCGCCTGAAACCTCTTACCGCAATGACCGCAATTGCATTCTGCCCAATGACCTCAAAACTG
  GAGCTCAGGCAGAATTACCGCCTCGAGAAGGCACGATGTGAACTGATCGTGAATATCGAGCTGCTGAAAA
  TCCAAAACCACGGGGGACAGACCATAAAGACACTCACCTCCTTTGCAATCGTTCGAAAGGACTCCGATGG
  CCAGGACTGGGAGACATGTACACGCTTCGCATCCGTTAGTATCGAGGATATTCTGAGAAGCAAACCGGCC
  GCGAACGGAACCTGCTGCCCACCTCGCGATGTGCATCATGACAGACCTACCTTGCAGTCCTCAAATTCCT
  GGACCAGGACCGAGTATTTTGAACCCTGGCAGGATGTGGTGGACGCCTACGTACCCATCAATGATAATCA
  TTGCCCTAATGATAGCTACGTAGTGTTTCAGACACTTCAGGGCCATGAGTGGTGTTCAAGGCTTAACAAG
  AACGATACTAAGAACTACCTCTCCAGTGTGCTTGCTTTCAAAAATGCTCTGTACGAGACAGAGGAGCTGA
  TGGAAACCATCGGGATGCGATTGGCTTCCCAGATACTTTCATTGGTGGGTCAGCGGGGGACCTCTATACG
  GAATATTGACCCTGCCATTGTGTCCGCCCTGTGGCATAGTTTGCCGGAGAAGTTGACCACTACTAACATT
  AAGTACGACATTGCTAGTCCGACTCACATGAGCCCTGCACTGTGCACAATTTTCATCCAGACAGGCACCT
  CAAAGCAGAGATTCAGAAACGCCGGTCTTCTGATGGTCAATAATATATTTACCGTCCAAGCCAGATACAG
  CAAGCAGAACATGTTCGAAAAAAAGATCTATGGATACGAGCACCTGGGACAGGCACTTTGTGAAGATGGA
  GAGATTCTGTTCCAGAATGCAGGACAAAAATTCTGTAGACCATTTACAGATAACAGAACAATCGTCTATA
  CAATGCAGGATCAGGTTCAGCGGCCCTGGTCCGTAACATGGATGGATTTTAATCTGGTGATATCCGATTA
  CGGCCGAGCCGTGATAGAAAATCTGACAGAATCAGCTATGAGCGCACATAAAAATGGACCAAGGTATCTG
  CAGATGGAGACCTTCATCAGCGACCTCTTTCGATACGAGTGCCATCGCGACAACAGGTACGTACTCGAGA
  AGAAACTTCAGATGTTCTACCCTACCACGCACATGAATGAGCTGCTGTTTTACCCTAGTGACCCCACTCT
  GCCCTCACCATACGGTAATGGACACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101531 representing NP_050271
  Red=Cloning sites Green=Tags
  
  MRPPRRSAPILVCAISMATALSNATVHRDAGTVESTPPPDDEDNYTAKYYDDSIYFNIYDGTNPTPRRRT
  LPEIISKFSTSEMSRLGGLKAFVPVDYTPTTTLEDIEDLLNYAICDDNSCGCLIETEARIMFGDIIICVP
  LSAESRGVRNLKSRIMPMGLSQILSSGLGLHFSLLYGAFGSNYNSLAYMERLKPLTAMTAIAFCPMTSKL
  ELRQNYRLEKARCELIVNIELLKIQNHGGQTIKTLTSFAIVRKDSDGQDWETCTRFASVSIEDILRSKPA
  ANGTCCPPRDVHHDRPTLQSSNSWTRTEYFEPWQDVVDAYVPINDNHCPNDSYVVFQTLQGHEWCSRLNK
  NDTKNYLSSVLAFKNALYETEELMETIGMRLASQILSLVGQRGTSIRNIDPAIVSALWHSLPEKLTTTNI
  KYDIASPTHMSPALCTIFIQTGTSKQRFRNAGLLMVNNIFTVQARYSKQNMFEKKIYGYEHLGQALCEDG
  EILFQNAGQKFCRPFTDNRTIVYTMQDQVQRPWSVTWMDFNLVISDYGRAVIENLTESAMSAHKNGPRYL
  QMETFISDLFRYECHRDNRYVLEKKLQMFYPTTHMNELLFYPSDPTLPSPYGNGHY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050271
• RefSeq ORF: 1848
• Locus ID: 1497092
• MW: 70.0 kDa