U73 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101512

Myc-DDK-tagged ORF clone of viral ORF for origin binding protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050252

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U73
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101512 represents NCBI reference of NP_050252 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACAGGTTCCAGAACGATACGCTGTTCTCAGAATGGTTCGGACAGTCACTGTCAGACGTCAGAT
  TCCCTGATAACGTAACCGTTTATTCCCAGGCAGATTCCGCTGTTAGCTTCGAAAACGTGAGACAGCCTAT
  CAAGCTGGTCAGAGCCGCAATGGGTTCCGGTAAGACTACTGCCCTGATTCACTTCCTGAAACAGGTGCCA
  AAGGAACTTAGCGTGCTGCTCATTAGTTGTCGGAAGACATTTGCTGCCGAGATCCTGCACCGCTTCACTC
  TCAACGGTCTCGAGGACTTCGAACTGTATTGTGACATCACAGAAAGGCAGATCAATAACCGCAAAGTAAT
  AGTCCAGATCGAGTCTCTGCACCGGCTCACCGAGAACTACGACGTGCTCATACTCGACGAGATTATGAGC
  ATTATCAAGCAGTTCTACTCTAAGACAATGACAAAGACAAAGGAAGTGGACTGTAAGTTTTTGTCACTGA
  TCAAAAACTCCAGCCACGTGATCGCTATGGACGCAACCCTGACACGGCATGTGGTGGAGTTCTTCGCAGC
  CTTTAAGCCAGACACACAGATCGCTCTGATTAGAAATACATTCGTGAGCGCAATGTTCAGTAATCGCGTT
  GCCTACTTTTGCGATACGTTCTTTGGCAAAGAGTTCTCATTCTTCGCACGCCTGGAAGACAAGCTCCGCT
  GGGATAAAAAGCTCTGTCTCTTTTGCAGCACAGTCCTCGCCGCCGAGTATATGCATGATCTGATTCGCAG
  CAGATTTTCTCTGAAAAAAGTGCTGCTGCTGACCTCTAAGCAGGGCAAGTGTTCCAGTATTGAGAGTTGG
  ATCCGGTATGACGTGGTGATCTATACCTCAGTCGTGACTGTGGGACTCAGTTTTGAACCCGTGTATTTTA
  GCTCCCTGTTCGTGTATATCCAGCTGGCAAAGGGTGGCCCAGACATGGTGTCCATCTTTCAGTCTATTGG
  CAGAGTGCGGAGGGTCATCGACGAAGACATCTATATCTATATGAATCCGGTTCTTATCAAGTCATACGAC
  CCGCTGGCTCCTATCGCCATGCCCCCATGCTCAGACTGGTCCGTCGCCGAACAGTCTATTATCAGTGAGT
  CCTGTATTGACTTCAGAGGAAAATGTAGTGGCGCGCACAAGTACAATTTTTGCAGCGTGCTCAAGTGCCT
  TTTTCGGTATCGGCATTACATTGAGAAGACGACCATCACTAGTTTGAGTGATTCTTTGTTTCTGCTGTGT
  TCCCTGTTGTGTGAAAACTCCATCAAGGTAGACATTGTCGGTAACGGATTTCCAATGCGGAAGGAAGTGT
  TCCTGTCATTTCTGCAAATTTTGGTTGAAGAGTGTCATTTTATTGAGAAAAAAATCACACTTCCTGGAGA
  CAACATGACTTTCCAGGAAATAATCTCTTCTAGGGAGACTATTATGAACGGGGACTTTTATGAGAACGGG
  AACCAGCTGCTGCACAAGGATTACATTACCGATATGGGCAAATTTAGGGCTACCTTCCTCTCTCCAGGCG
  TCGATATTTTCATTGCCTCTGATATCGTCTCCGATCTGAAGAATGAATCAAAGAGATACGTGTTCGTGAA
  TGTGTGGCTCCAAAAGTGCGTCAGCGCGGGCGTCGAGTCAACCCGAATCGAGCGGGTGTTTAATGAGCGG
  ATCAAAAGCTACGTGTTGCCTAAATCCTTCTTGTGTGACGAGTACTTCGTGCTCGGCGACATTTCCGGTG
  TGTACGAGTGGGGCATGCTGATCGACCTTGCATTCCTGGCCGAGATGATCCGGAAGGACTTGAAGCTCAA
  GAGCTGCACTGACACTACCACGGACATTAGTGAAGACGACCTGCTGCTCTGCGCCGCCAGGCGCAGCAGC
  GATATCCTCCAGATAATGCAGCTTGTGTTCACCGTCCATGTGCAGTTTTTCCAAAGGTATAGCCTCCAAA
  CCCTTCAATTGTTCAACAAACTGCGGGGCATGCGGATCGTGACAGGGGTGTTCAGCATAGAGAAGTTCTC
  CATAAGTATTTTGCGCCTGTTCTTCAAGTGTGCCTTTAATATGACTCTGTCTGCCTCCAAACCAAGGTAC
  ATCCCTGGAAAAGCATATAGGAACCTGACTAAAAATGATCTGGAAAACATGTTGGATAACTGGGAGATCA
  GCCGGACAAACCTGAAGACTTGCAAGGAACTTCGGAAAGCCCTGACCGAAGCTAGCAGGGCTCGAAGAAA
  GCAGACAATTTACAAACTCCAGGGTAGCGATATCTCACTGTCAGTGTCCGAGGTCGGCGTTTTCGGGCAA
  CATGCCTCACCAGGTGTGTGCGTGAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101512 representing NP_050252
  Red=Cloning sites Green=Tags
  
  MENRFQNDTLFSEWFGQSLSDVRFPDNVTVYSQADSAVSFENVRQPIKLVRAAMGSGKTTALIHFLKQVP
  KELSVLLISCRKTFAAEILHRFTLNGLEDFELYCDITERQINNRKVIVQIESLHRLTENYDVLILDEIMS
  IIKQFYSKTMTKTKEVDCKFLSLIKNSSHVIAMDATLTRHVVEFFAAFKPDTQIALIRNTFVSAMFSNRV
  AYFCDTFFGKEFSFFARLEDKLRWDKKLCLFCSTVLAAEYMHDLIRSRFSLKKVLLLTSKQGKCSSIESW
  IRYDVVIYTSVVTVGLSFEPVYFSSLFVYIQLAKGGPDMVSIFQSIGRVRRVIDEDIYIYMNPVLIKSYD
  PLAPIAMPPCSDWSVAEQSIISESCIDFRGKCSGAHKYNFCSVLKCLFRYRHYIEKTTITSLSDSLFLLC
  SLLCENSIKVDIVGNGFPMRKEVFLSFLQILVEECHFIEKKITLPGDNMTFQEIISSRETIMNGDFYENG
  NQLLHKDYITDMGKFRATFLSPGVDIFIASDIVSDLKNESKRYVFVNVWLQKCVSAGVESTRIERVFNER
  IKSYVLPKSFLCDEYFVLGDISGVYEWGMLIDLAFLAEMIRKDLKLKSCTDTTTDISEDDLLLCAARRSS
  DILQIMQLVFTVHVQFFQRYSLQTLQLFNKLRGMRIVTGVFSIEKFSISILRLFFKCAFNMTLSASKPRY
  IPGKAYRNLTKNDLENMLDNWEISRTNLKTCKELRKALTEASRARRKQTIYKLQGSDISLSVSEVGVFGQ
  HASPGVCVSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050252
• RefSeq ORF: 2340
• Locus ID: 1497073
• MW: 89.6 kDa