U4 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101449

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp009 [Human herpesvirus 6], codon optimized for human cell expression, NP_050186

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101449 represents NCBI reference of NP_050186 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGCTCGACCACGACATCTACAAGGGTCCTGTTCGAGAAAGGGTAACATACACTATACCCAATC
  ACCCCTATCTTTCCCTCACTGTTCACCACAGTCGCGAACTGGATGTGGACCTCAAAGATATTACAGAAGA
  AATGATCATTGACAGCGGGACCCTGACCGCTGAGGACCTGTTCATGACTAGAGGACTCAGATTCTGTGAC
  GACAGTGTGCTTTGGGCTGCCCTGGCTGAAAAGGTATCCGTGGAGTTCCAGCGCCGGGGAAAAACTACTA
  TGGATTTGTCAGCATTTATGTCCCTCCTGGAGCGGCTCACTTTTGAGGACGAGACAAGCGCCTTTTACCG
  GCTGCTGACCAAGTGTACCGCGCTCGCCCACTTCAGCATACTGGAGTACATCGTGGACGGCGAAAAGAGA
  GATACTATCTCAGCCCATTTTAACCGGCTCCTCGAATTGCTGGACTCTCTGTTCCTGCAATTCCTCATGC
  TTAGGAACCGATCTGAAAGTAATACGTTGCTCACACTTTTCGACATCCTGCCTAACCCAAAGGAAATCTA
  TGGAGATGCTGCAAGCCCCACGTCCGTCCTCTTGAAGCACTTCCTGAGCGACTACATTTTTGTGGCCTAT
  GCCTCCTCCTACAGATTCGTGTCTAGCGTATTGTGCTCTTGCGATCAGTGTCGCGCCAGCTTGTTCCGCA
  TCTATCTGGGTAAGAAAGATAGCCGGGCAGTGAATATTTCTGACATCTCCGAGTTCGATCCCGCCGACCT
  CATCCTGGGTCAGCTGCACCTGGACGCTAGGGAAGCCGTTGCCTTGCGCGCTGAGATTGACCGGGACCTG
  GGATCTTCTCTGATCCTGAATAGTATGGAGAGAAGGCACCTCCCAATCCTCCACGACAAACAGCTCGGGC
  GCGGACATTTTGAGAGGAACATTCTCAAGGTTTATTGTAATATTGTCCTGTGCCTGTTTCTCTTGCGCCG
  AGTAAAACAAAGAATCGTCTCCGATCTGACAGCTATTGCGAGGTTCTTCGTCAAAGCAATTTCTGAGATG
  GAACGCTGCATCGATACTGTCTCAGGATTGCACGGTGTTAGAATAAAGCTTCTCGTGGTGTCCAGTCAGT
  TTGAGATAAAAGACCCAATGCGCGTTCCACGGCTGTGCTTCTTTTTCCTTGAGATCGTAACTATCCTCGT
  TTCCGGCTACGATAAGGAGCGGCACCGAGTGCGGGCACTGCTTGAGCATCTGTGTCTGAAAAAGATTTGT
  CTTGGCGACCTGTGTGCAAGCATGAGACTCGCACGCGAGATCCGCCACGATGTGTTGGAGGGCTTTCTGA
  ACACCCTGGAGCTGTCTTACATGAGCAACCCAGTGCGGTTTTTCCGATTTCACGATTGCAACTTGTATGG
  CACACACATGTGGTCCGGCGTTTACCCTAACGTCGTTCCTTACGCAGTCAGAGGGGGGGGGTCATTCGAC
  ATCAGGTTGCAAGAGCATCGCAGACGGCAAAAAAGGATAGTCCTCAGAAGGAGGTTGATTCGCAGGGTCC
  AGCAGAGGGGATTGGACATCCGAGAGGCAAGACGGGTCCCGAAGCTCGCTTGTGTGACTTTTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101449 representing NP_050186
  Red=Cloning sites Green=Tags
  
  MELLDHDIYKGPVRERVTYTIPNHPYLSLTVHHSRELDVDLKDITEEMIIDSGTLTAEDLFMTRGLRFCD
  DSVLWAALAEKVSVEFQRRGKTTMDLSAFMSLLERLTFEDETSAFYRLLTKCTALAHFSILEYIVDGEKR
  DTISAHFNRLLELLDSLFLQFLMLRNRSESNTLLTLFDILPNPKEIYGDAASPTSVLLKHFLSDYIFVAY
  ASSYRFVSSVLCSCDQCRASLFRIYLGKKDSRAVNISDISEFDPADLILGQLHLDAREAVALRAEIDRDL
  GSSLILNSMERRHLPILHDKQLGRGHFERNILKVYCNIVLCLFLLRRVKQRIVSDLTAIARFFVKAISEM
  ERCIDTVSGLHGVRIKLLVVSSQFEIKDPMRVPRLCFFFLEIVTILVSGYDKERHRVRALLEHLCLKKIC
  LGDLCASMRLAREIRHDVLEGFLNTLELSYMSNPVRFFRFHDCNLYGTHMWSGVYPNVVPYAVRGGGSFD
  IRLQEHRRRQKRIVLRRRLIRRVQQRGLDIREARRVPKLACVTFI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050186
• RefSeq ORF: 1605
• Locus ID: 1497007
• MW: 62.0 kDa