U41 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101482

Myc-DDK-tagged ORF clone of viral ORF for major DNA binding protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050222

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101482 represents NCBI reference of NP_050222 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACGAAAATGAAACTGTCGTTTCCGCTCCCGTCTCCACTGCCGCCTGGATCTACGTCTTTCCAA
  AGGAAAAGGAACTGCTCGACGTCCTCTCCGTGCTGAGCCTTATGGAACGGAACAGCCCAGTCGTAATATC
  ACCCCTGTTGATGAATCTGACTGTAGAGAATGACTTCAGCACCACCGTAAAGACACCTATCACCAATTTC
  GGGGGAACCATCCTCACCAAGATTACCTCCTTCATGCCTGTGTGCTTCTTCTTTCACGGGACAGAGCAAT
  TGGTTGGGATGGCCGAGGATCATGGCGATCTTATCCGGTTGTGTGAGCAGACCCGACAGAAATTCCATTT
  GCAGTCTTTTGAGGTTCCCACTGCTAGAAAGGTTATCGACATCAAAGCGTTGTGCTCCGCCGTAGGCAAA
  GACGCCGATTCTGTGATCTGTCACGTAGCTTGCGGCAACGGATTCAAGGAGCTGCTGTTTGCCGGCTTGC
  TCATTCCTTGCGTGGAAGAGCAAATTCAGGTTCAAGTGGGCGAGTATTCTTGCGTAAAGATCCCGTTGTA
  CAGTGCTACACTGTTTGAAACCGAGGAAACCATCTCCCTGTCCAGTTGTACTGAATTCATACAGGAACGG
  GGGTTTTTTCTTCCCGCCTTGTCAGAGACTCTGTTCTATTATGTGTTTACCTCTTGGGGTACAACCCTGC
  GATTCAGTAATACCAAAGAGTTGATCGACGCAGGGCTGAAGCAGTTTACCCAGGATGGCGAGCAGACAGT
  CAAACTGGCCCCTCACAAGACCTACCTCGGAATCAGCGGACAGAAAATTAGCGCAGTTGAGAAGGACTTT
  TTGATGTTGGTCGACAGTGTCGTGACCGAGCTTTCTTTCAGTCACGTTGCGGAGTACCTGGACAGCGTCT
  ACGACCCATCCCAAATTATGAATTTTAACGATTGGCCTATTATTCGCAACAGCGAGACGCATGCAGAAAG
  GATGGCCCAGCTGACCAACCTGAAGCTGCATCTGAGCTCCCATCTTGCTGTACTGATTTTCGCACCCAAT
  AGCATATTGTATTGCTCAAAATTGGCTTTCATCCCCAACGTCAAACAGGCCTTCAATTCAGTGATGACTC
  AAGAGCTCCTGCTGCGCTCTCTCTCTTTCTGTAACGCTCTGTCTAGCCTGACCGAAGACGTCTACAATGA
  CAATCGGAAGATTATCAAGTGCGATAGCACCAGCGGTAAAGATGATAAATTTTCTGCTAATCACTTGGCC
  TATGCCTGTGCTACCTGCCCCCAATTGTTGAGTTATGTTGTCTGGAACCTCAACCGGATGTCCGTGTACA
  ACGCGGGAAATGCGTACACGGAGATTTATAACCATCTGGTGAACTGCTCTGCAAATCTGTGTGAGTTCTG
  TGATGGTAAGTGCTGCCAGAGCTGCATAGGCACTGCCATGGTTCGGGTGGGGACGAGACTGCCCGCCATT
  CCCAAGAATGTGAAGAAAGAGCCCTTGGTGATGTCCATGTTTAGCCGGTACTACGCTGAGGTGGATATCC
  TCGGATCCTTTGGCCGGAAGCCGGTGTCCGAACTGAAAGAAATCGGCAAAGACCAGCAGAATACACTCAG
  TCTCGATCGCGGTAAGTTTGTCTCCCAGATATTCGATTACTGTAAGAAGAATTCTCTCATCGATCCAGTC
  ACCGGAGAGGATACGTTTAATGTGCGGAGTAAGAAAGACTTTGTCTCCATTATTCATGGGCTGACTCAGT
  GTATTGAGGAGTGTGTGAGTAGGTGCATCGTAGAGATGCGCAGGACCCAGACCCCCAGAGAACAGATCGA
  GAACTGCTTGCAGAGCTTTAACGTCGACACCACCCCATACGCTACAGCTTTCTCACCCTTTCTGACATTC
  GCCTACTACAAAGTGATACTGACTGTGCTGCAGAATCTGGCCCTGATCGTGGCAAGTGGGCACGTCGTGG
  ATCGACCATGCACCGGGAACAGTATTAGCAAGTGGCTGGTGCAGCAATACCAGTCCTTGTACGGCACCTT
  TCACTCATCCTATCTCAAGAAGGGCTTCCTTAATACCCGGACCGTGAAGGTTGCTTCAAACGTCGATATG
  GAGCAAATCCTGGACTGTGACCTGTATAAATCTGGGAAGTATGTCAAGACCACCATCCAAGCGAAACTCT
  GTAGGCTCTCCATGCAGTGCCTCAGGGATTTCCGGATTAAGAATAGACCTTTTAACAAATCCTCCAAGAC
  GGCGCACAATAACCCTTATTTTAAAAAGAATGTAAAACATAAAAAGAACCCTCTGTCAGGTTGCATTTCC
  TTTTTGCTGTTCAAATACCACGACAAGTTGTTCCCTAATGTTAAGATCTCATGTCTCGAGCTCTGGCAGC
  GCTTCCTGCTGAATAACGTTCCCAAGACACTGGACATAGGCAATCCTGAGGAAGTGAAAACATTCATTAA
  GTTTGCCTTTTCCATCACTAATACTTATGATGAGATTGACATCATTGATATACAGCCGGAATGCCTGTCA
  ACATTTATCGATTGCTATTTTCACAATAAATTTCTCAGCGCCCTGGGATTCCATGACTATTTGACTTCCT
  TGCACGGACTCACCTCTAAACTGGTTACCCAGAACCCCGTATTGTTCCCGGTGGTACTTGACAAGCAGCC
  CAAATTTTCAAGTATACAAGAGTACCTGGTTTATGTGAAGAAGTTGGTTCTGGACGGTGTCCCTAATCCC
  GTGATTGCTAGCTTGTCTAAGGAGCCAAATTTCGGCACTATTTTTACCAGTAGGAGTTTGGTTACCTTTG
  GTCTGACATTGGAGAAGTTTGTGAGTCTCGCAAACCGCGAGTATTTTCAGTTTGGGCAACTCGGCTGGAT
  TGGAGGATCCGGGGTCGACCGGAACCTGAATCCAACATCATCTGCCCTGCAAGATTTTAGATTTATGAGA
  CAGAAGACTATTATCGCCACAAAATTCTCTGAGGTGATCGTGAAGAAGGTACGCAGGGAGACTATAATGT
  TCGATACCGAGGTAGTGAAAGGCAAAGTGCTGTCAATTGTGGAGAACCTCACAAACGATATAGATCCCGA
  GTTGCTGATCATTGCCGAGGTTATGCGGGACCGGGAGGATAAGCCCACGATGGATGACATGCTGTTCTTT
  GTAGACGGGAGAGAGGCCCTCGCCGCCTCAATCATGCTGAAACTGAACCACCTGGTGGATATGAACGTGC
  GGGATTTTAGCATCGCCAATCTCCAGTCCGTATTCGAAGCAGTGTCCTCTAACGATGCTCCTGTCTACGA
  TTTTTCTGAAATCCTGGCTGAAGAGGATGACCAGGCCAGCGGAGTTCTTAAGTGTGACGAAACGGAGACC
  GAAACTGACGAACCTATGACTAAAAAGAACAGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101482 representing NP_050222
  Red=Cloning sites Green=Tags
  
  MADENETVVSAPVSTAAWIYVFPKEKELLDVLSVLSLMERNSPVVISPLLMNLTVENDFSTTVKTPITNF
  GGTILTKITSFMPVCFFFHGTEQLVGMAEDHGDLIRLCEQTRQKFHLQSFEVPTARKVIDIKALCSAVGK
  DADSVICHVACGNGFKELLFAGLLIPCVEEQIQVQVGEYSCVKIPLYSATLFETEETISLSSCTEFIQER
  GFFLPALSETLFYYVFTSWGTTLRFSNTKELIDAGLKQFTQDGEQTVKLAPHKTYLGISGQKISAVEKDF
  LMLVDSVVTELSFSHVAEYLDSVYDPSQIMNFNDWPIIRNSETHAERMAQLTNLKLHLSSHLAVLIFAPN
  SILYCSKLAFIPNVKQAFNSVMTQELLLRSLSFCNALSSLTEDVYNDNRKIIKCDSTSGKDDKFSANHLA
  YACATCPQLLSYVVWNLNRMSVYNAGNAYTEIYNHLVNCSANLCEFCDGKCCQSCIGTAMVRVGTRLPAI
  PKNVKKEPLVMSMFSRYYAEVDILGSFGRKPVSELKEIGKDQQNTLSLDRGKFVSQIFDYCKKNSLIDPV
  TGEDTFNVRSKKDFVSIIHGLTQCIEECVSRCIVEMRRTQTPREQIENCLQSFNVDTTPYATAFSPFLTF
  AYYKVILTVLQNLALIVASGHVVDRPCTGNSISKWLVQQYQSLYGTFHSSYLKKGFLNTRTVKVASNVDM
  EQILDCDLYKSGKYVKTTIQAKLCRLSMQCLRDFRIKNRPFNKSSKTAHNNPYFKKNVKHKKNPLSGCIS
  FLLFKYHDKLFPNVKISCLELWQRFLLNNVPKTLDIGNPEEVKTFIKFAFSITNTYDEIDIIDIQPECLS
  TFIDCYFHNKFLSALGFHDYLTSLHGLTSKLVTQNPVLFPVVLDKQPKFSSIQEYLVYVKKLVLDGVPNP
  VIASLSKEPNFGTIFTSRSLVTFGLTLEKFVSLANREYFQFGQLGWIGGSGVDRNLNPTSSALQDFRFMR
  QKTIIATKFSEVIVKKVRRETIMFDTEVVKGKVLSIVENLTNDIDPELLIIAEVMRDREDKPTMDDMLFF
  VDGREALAASIMLKLNHLVDMNVRDFSIANLQSVFEAVSSNDAPVYDFSEILAEEDDQASGVLKCDETET
  ETDEPMTKKNRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 3396 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050222
• RefSeq ORF: 3396
• Locus ID: 1497043
• MW: 127.8 kDa