U44 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101485

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp050 [Human herpesvirus 6], codon optimized for human cell expression, NP_050225

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101485 represents NCBI reference of NP_050225 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTTGGTTACTGGGGAGTCATGGGCAACTGCTTCAGACGACTGTCTCTCTTCGGCCAGCCCACTC
  AGGCCAATTACGAGATGCTGTGCTCCAGCGATGATCTGGAATCAGAGGAATTGACCTACTTCCTCGATAT
  GACATACAAAGATTTCGGCGTGTATCAGAACGATATCATAAGTCACCAGAAGGATACTGAAACCATGAAG
  ACCCTGCTCGGACTGCTGCCCATGTACAAGAAAACTAAGCTCAGGCACACCATCATGGAAAGGTGCCTGT
  CAAATTGTCCCAACCACGTGAAGGATGCGCTGTGCGTGGAGTTGATGAAAGCGGAGAAAATTCTGCAGAC
  AATGGATGTGGTCTTCATGAAAACTCTTATCGGCGAGTTCTCTATGTGTACCGATAACCTCAATCAGCTC
  TTGAATAAGTTTGCAACTGATCAGTCCACCCTGAGTGACGTGGAGAAAATCAACTCACTGATCGAGATCG
  ACGGAGAGAATTCCAAGCGGCTGCTCGTCGAGCTCGACCCTATCCTGCATGAGGAGACGGGGCTTTACCA
  GGCATTGCCGAACGTCGTCACCGAAGCCCCAAGCGAAAAGGTTAAGTCCATCCATGTCGAGAGTGAGGGC
  GAGAGTGTGTGGTCTAGTGTGACAGAAGGCGGCATCATGAAACAAGAGAAAGGTACCGGAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101485 representing NP_050225
  Red=Cloning sites Green=Tags
  
  MPLGYWGVMGNCFRRLSLFGQPTQANYEMLCSSDDLESEELTYFLDMTYKDFGVYQNDIISHQKDTETMK
  TLLGLLPMYKKTKLRHTIMERCLSNCPNHVKDALCVELMKAEKILQTMDVVFMKTLIGEFSMCTDNLNQL
  LNKFATDQSTLSDVEKINSLIEIDGENSKRLLVELDPILHEETGLYQALPNVVTEAPSEKVKSIHVESEG
  ESVWSSVTEGGIMKQEKGTGV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 693 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050225
• RefSeq ORF: 693
• Locus ID: 1497046
• MW: 26.2 kDa