U76 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101515

Myc-DDK-tagged ORF clone of viral ORF for putative viron protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050255

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U76
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101515 represents NCBI reference of NP_050255 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATAGGGCAAGCGCCAACTCTCCACTGAACTCCGTCAGCGGGAGTATGATGTGGCGGAATCAGTCTA
  GCGGTCGAAGACCGAGTAAGAGGCTCAGTGATAATGAGGCTACATTGAGCACCATCAATAGCATCCTGGG
  GGCCGAGGACATGCTTAGCAAGAACCTTCTTAGTTACCTTCCTCCTAATAACGAGGAAATTGACATGATC
  TATCCGAGTGAACAGATTATGACCTTTATCGAGATGCTTCACGGTCACAAGAATTTTTTTAAGGGTCAGA
  CCATCCATAACGCATTGAGAGATTCTGCGGTTCTGAAAAAACAGATCGCATATGGAGTCGCCCAGGCACT
  GCTGAATTCAGTCTCCATCCAGCAGATCCATGATGAGTGGAAGAGACATGTGCGGTCCTTTCCATTCCAC
  AATAAGAAGTTGAGTTTTCAAGATTATTTTTCAGTCTGGGCTCACGCCATCAAACAAGTGATCCTTGGCG
  ACATCAGTAACATCATCAACTTTATACTGCAGTCCATCGACAACAGCCATTACAATCGATACGTAGACTG
  GATCTGCACAGTGGGGATCGTACCATTCATGCGAACCACCCCCACCGCTCCAAATCTGTACAATCTGCTG
  CAGCAGGTGAGTAGTAAACTGATTCACGACATAGTTCGACACAAGCAGAATATCGTGACTCCAATTCTCC
  TGGGTCTGAGCAGTGTGATCATTCCTGACTTCCATAATATAAAAATCTTCCGGGACCGGAACAGCGAACA
  GATCTCCTGCTTCAAAAATAAAAAGGCAATCGCCTTTTTTACCTACTCTACCCCCTACGTGATCCGCAAT
  CGACTCATGCTGACTACCCCCCTGGCTCATCTGTCTCCAGAACTGAAGAAACATAACTCACTGCGAAGGC
  ACCAGAAAATGTGCCAGCTGCTGAACACCTTCCCAATTAAGGTGCTGACTGCCGCCAAGACCGACGTCAC
  CAACAAAAAGATCATGGACATGATCGAGAAGGAGGAAAAGAACAGTGACGCCAAGAAGTCTCTGATCAAA
  TTCCTGCTGAACCTTTCCGACTCCAAATCCAAAATCGGTATCCGCGACTCTGTCGAGGGCTTCATTCAGG
  AGATTACTCCCAGCATTATCGACCAGAATAAGCTTATGCTTAATAGGGGGCAATTCCGAAAAAGATCCGC
  GATCGACACGGGGGAGCGGGACGTGAGGGACCTGTTCAAGAAACAGATAATCAAATGCATGGAAGAACAG
  ATCCAAACTCAGATGGATGAGATCGAGACACTCAAGACCACCAACCAGATGTTTGAGAGGAAGATTAAAG
  ACCTGCACAGCCTGTTGGAGACTAATAATGATTGTGATAGATATAATCCGGATCTGGATCATGACCTGGA
  AAACTTGAGTCTCTCTCGCGCTCTTAATATCGTACAGAGACTGCCCTTCACGTCAGTGTCTATCGATGAC
  ACCCGGTCCGTGGCAAATAGCTTTTTCAGTCAGTATATTCCCGACACCCAATATGCTGACAAGCGGATCG
  ATCAGCTCTGGGAAATGGAGTATATGAGGACATTTAGATTGCGCAAGAATGTGAATAATCAAGGGCAGGA
  GGAATCTATAACATACTCTAATTATAGTATCGAGCTGCTGATCGTGCCCTTTTTGAGGCGCCTTTTGAAT
  ATATATAACCTGGAAAGCATACCCGAAGAGTTTCTCTTCCTTAGTCTTGGAGAGATCTTGTTGGCAATCT
  ACGAGAGTTCAAAGATTAAGCACTACCTTCGCTTGGTGTATGTGAGAGAGCTCAATCAAATCTCCGAGGT
  CTATAACCTGACTCAGACCCACCCCGAGAATAACGAGCCAATCTTCGACAGTAACATTTTTAGTCCCAAC
  CCCGAAAACGAAATTCTCGAGAAAATCAAGAGGATTCGGAACCTGAGGAGGATCCAGCACCTGACAAGGC
  CGAATTATCCCAAGGGCGATCAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101515 representing NP_050255
  Red=Cloning sites Green=Tags
  
  MHRASANSPLNSVSGSMMWRNQSSGRRPSKRLSDNEATLSTINSILGAEDMLSKNLLSYLPPNNEEIDMI
  YPSEQIMTFIEMLHGHKNFFKGQTIHNALRDSAVLKKQIAYGVAQALLNSVSIQQIHDEWKRHVRSFPFH
  NKKLSFQDYFSVWAHAIKQVILGDISNIINFILQSIDNSHYNRYVDWICTVGIVPFMRTTPTAPNLYNLL
  QQVSSKLIHDIVRHKQNIVTPILLGLSSVIIPDFHNIKIFRDRNSEQISCFKNKKAIAFFTYSTPYVIRN
  RLMLTTPLAHLSPELKKHNSLRRHQKMCQLLNTFPIKVLTAAKTDVTNKKIMDMIEKEEKNSDAKKSLIK
  FLLNLSDSKSKIGIRDSVEGFIQEITPSIIDQNKLMLNRGQFRKRSAIDTGERDVRDLFKKQIIKCMEEQ
  IQTQMDEIETLKTTNQMFERKIKDLHSLLETNNDCDRYNPDLDHDLENLSLSRALNIVQRLPFTSVSIDD
  TRSVANSFFSQYIPDTQYADKRIDQLWEMEYMRTFRLRKNVNNQGQEESITYSNYSIELLIVPFLRRLLN
  IYNLESIPEEFLFLSLGEILLAIYESSKIKHYLRLVYVRELNQISEVYNLTQTHPENNEPIFDSNIFSPN
  PENEILEKIKRIRNLRRIQHLTRPNYPKGDQD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1986 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050255
• RefSeq ORF: 1986
• Locus ID: 1497076
• MW: 77.2 kDa