U70 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101509

Myc-DDK-tagged ORF clone of viral ORF for Alkaline exonuclease [Human herpesvirus 6], codon optimized for human cell expression, NP_050249

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101509 represents NCBI reference of NP_050249 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTTAACCAGATCTCTGAAACACTCTCCGCAGTGGCTGAAGAGGAGCCTCTGACTATGTTTCTGC
  TGGACAAGTTGTACGCTATCAGGGAAAAGATTAAGCAGGTGCCATTCTCTATTGTGCGCCTGTGCCACGT
  ATACTGCATGCTCATTAAGTATAATGCAAGCAACAATAACTGCATCTTGGGCCGAAAGCTTATCGAGGAA
  ATGCAACAGTTTCTGTGCGGAGCCAGAGTGGATGGATCTGAGGACGTGAGCATGGACATGAGTGAATTGT
  GCAAACTGTACGACTACTGCCCCCTGCTCTGCTCAGCCTTGTGCCGGGCTCCCTGTGTCTTTGTCAATAA
  ACTGTTCAAAATCGTTGAACGGGAGACGCGAGGGCAGAGTGAGAATCCCCTTTGGCATGCCCTGAGACGC
  TATACAGTCACTGCCACAAAACTTTATGACATCTACACTACAAGGAACTTCCTCGAGCACAAGGGCCAAC
  AGTTCTTTGGGGAAGCAGTTATATACGGGGCGAAGCATGAGAGGGTGATACGCCATCTTGTGGCCATCTT
  CTACGTGAAAAGGGAGGTTAAAGAAACCCTGGGCTTGTTGCTGGATCCCTCTAGCGGAGTTTTCGGGGCG
  AGTCTGGACGCCTGTTTTGGAATATCATTTAACGAGGATGGATTCCTTATGGTCAAGGAAAAAGCCCTGA
  TTTTCGAAATTAAGTTCCGCTACAAGTACCTGCGGGACAAAGAGGATCACTTCGTTAGTGAGCTGCTGAA
  GAATCCTACGGAGAAGTCTTTTTCAGACTTTATTCTGAGCCATCCAGTGCCTGCGATTGAATTTCGAGAG
  CGGGGAAAGATTCCCTCTAGCCGCGAGTATTTGATGACCTACGATTTTCAATATCGGCCTCAGCGGAAGC
  TGAGAACCTGTCCGACTCCAGCAATTCTGACACCCCACATCAAACAGCTGCTGTGTCTGAACGAGACCCA
  AACTAGTACGGTGATAGTCTTTGACTGTAAATCCCACCTGTCCGAACAGAAACTTAGCGTGTTCCAGAAA
  GCTGTATTTACAGTTAACGTGTTCGTGAATCCCAAGCATCGCTATTTTTTCCAGTCACTGCTTCAGCAGT
  ACGTCATGACCCAGTTCTACATCAACGATCACTCCAACCCAGAGTATATAGAATCAACAGAGGTACCATC
  CGTTCACATTGTCACCGCACTTTTCCGACGGAGGACCGAAGAGGAGAGATCTCTGCATCTGGTGATCGAT
  GAAACGGAATACATTGAGGAGGAGATACCGCTTGCTCTGATCGTTACACCCGTTGCTCCTAATCCAGAAT
  TCACATGTCGAGTGATAACTGACATTTGTAATTTGTGGGAGAACAACATTTGTAAACAGACTTCCCTCCA
  GGTGTGGGCTCAGAGTGCCGTAAATCAGTACCTGGCAGCATGCGTTCGGAAGCCAAAGACGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101509 representing NP_050249
  Red=Cloning sites Green=Tags
  
  MDLNQISETLSAVAEEEPLTMFLLDKLYAIREKIKQVPFSIVRLCHVYCMLIKYNASNNNCILGRKLIEE
  MQQFLCGARVDGSEDVSMDMSELCKLYDYCPLLCSALCRAPCVFVNKLFKIVERETRGQSENPLWHALRR
  YTVTATKLYDIYTTRNFLEHKGQQFFGEAVIYGAKHERVIRHLVAIFYVKREVKETLGLLLDPSSGVFGA
  SLDACFGISFNEDGFLMVKEKALIFEIKFRYKYLRDKEDHFVSELLKNPTEKSFSDFILSHPVPAIEFRE
  RGKIPSSREYLMTYDFQYRPQRKLRTCPTPAILTPHIKQLLCLNETQTSTVIVFDCKSHLSEQKLSVFQK
  AVFTVNVFVNPKHRYFFQSLLQQYVMTQFYINDHSNPEYIESTEVPSVHIVTALFRRRTEEERSLHLVID
  ETEYIEEEIPLALIVTPVAPNPEFTCRVITDICNLWENNICKQTSLQVWAQSAVNQYLAACVRKPKTP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050249
• RefSeq ORF: 1464
• Locus ID: 1497070
• MW: 56.7 kDa