U34 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101475

Myc-DDK-tagged ORF clone of viral ORF for putative virion protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050215

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101475 represents NCBI reference of NP_050215 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAATGTCTTGAAGGAGAAAATGTACGACGAGCTTTTGAGCGCAACCTGCAGGATTCTGAAGCTCG
  GAAGCCATGATTACAGGATGACCGAGAGAAACCTGCTCAGTAAAAATCCCAAGTTCCCTCTGTGCGACAT
  CATCTTGAAACTGGACTACGCTTATAATCTCGAGTACCTGCTCAGCCTTTGGGAGCACGTCACGAAGCAG
  GAGCCTCGCTTCGTATTTAAGAATACTGGTGGGGCCGTTTCCATGTCATGCTACCTGCACGCACCCGTTA
  AAGCCGAGGGTCACCATGCAGTAAGAGAATGCAATATTTTGAGAGTCAATGAGTGCCTTACTGTGCGGAT
  GTCTGACATCGTTGCCATGAAACCCTCAACTTTCGCTGTATTCACGAAGTGTATCATTAGACGCAACAGA
  GACGAGACTTACGTTGTGGAATTCGTCGCGTTTGGCCCCGAAAATGAATCCGAATACATTTCATTGTTGA
  AAGCTATTTTTCTCAAAAAATGCTCCATGGGAAAGCAACATCTGGAGTCAAACCGCTTCTGCCAGGGCCT
  CAGGAGACGGAGTAGTCATGTGCTCGAAAAGGGACAACTCGGATCCAGTGGAGAGATAGCAAACAAGGCT
  TCTGCAGTCGTGACCAGCCAAGAGAGCATTAATCAGTTTTACGAAAAGGAGAAAAGCTTCCTGTCTGGTG
  TCAAATTCTCAAGGCTGAGTGAACGCCATTGCAGAGTGGCAATCGTCTCCATTTGCTTTCTGTTGGCTCT
  CTATTTTTGCTACGTGCTTCTGAAAAAGACACCCACCCCAGCGTCAGGCCCAGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101475 representing NP_050215
  Red=Cloning sites Green=Tags
  
  MANVLKEKMYDELLSATCRILKLGSHDYRMTERNLLSKNPKFPLCDIILKLDYAYNLEYLLSLWEHVTKQ
  EPRFVFKNTGGAVSMSCYLHAPVKAEGHHAVRECNILRVNECLTVRMSDIVAMKPSTFAVFTKCIIRRNR
  DETYVVEFVAFGPENESEYISLLKAIFLKKCSMGKQHLESNRFCQGLRRRSSHVLEKGQLGSSGEIANKA
  SAVVTSQESINQFYEKEKSFLSGVKFSRLSERHCRVAIVSICFLLALYFCYVLLKKTPTPASGPVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050215
• RefSeq ORF: 828
• Locus ID: 1497036
• MW: 31.4 kDa