U23 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101539

Myc-DDK-tagged ORF clone of viral ORF for glycoprotein [Human herpesvirus 6], codon optimized for human cell expression, NP_050203

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101539 represents NCBI reference of NP_050203 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAAGTCTGAGTTCAACGCCAAATCTTGTTTCCTGATGATCGGCATCTGCGTTTTCAACCTGAATT
  CATCAAGTTGCATCATCAAGACAAACGCGGAGCACTGCAGCAAGCGGTTGTTCAGCTTCTGCTATCTGGG
  AGTCGGAGTGATAAAGAAGCCACTCCATCTGGGTAATAGAAAGAACATGCTGTTCTTGTCCTTCCTGTTG
  GTGTGTCTGTGCGAAGAAGTCCAAATGCTGAATCTCACAACAACTGAGGTGTCTGCAACTGAATTCGCTA
  GCATTGCTTCCAAGAATATGGAAACTAATGTCTCTACCAGCAGCGACTATTTGACCGGAAAGAGTGAGAC
  GACTTTTAGTGCGAATCCAGAGACTTGGGGAAAGAACGTTACTGAAATTTCCATAGCAAGCGTGGCATAC
  CTGAATCAGTCATCAATGGTAACCAGTACCCTGGCTGTTGGCACGACAAACAGATCAAGCGGAAATAACG
  TGAATGTGACTACTTCCTCTTTCCCTACCGTGAAGGGAGATGAAGCCCAGGATATTGAGACGTTTTTCAC
  GGTTATCCTCGCGAGCACTCTCAGTGATGTGAGCGAAAAAACCCCACAAGGTCTGCCAACCAAGTCTACC
  CCCAAGAAGACTGTTCAGGCTCTGTGGGAAACCGACACAGTGCAGGTACCTGAGCTGACTGATACTAATG
  AAGGCGACGAGGAATACTTTAAAGATTTTCTGAGCTCTCTCGTGATCTGGATCTGCGGAATCTCCTTTGT
  TGGCGCCTTCATCATAGTTATTGTAATTCTCTATAACTGGTATAAGAAGGACAAACAGCGCTCTCTGCTC
  TGGGATGAAGAAAACAAACCCGATGTCCAGATTAGGCGCGATGCTAAGACCTGTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101539 representing NP_050203
  Red=Cloning sites Green=Tags
  
  MRKSEFNAKSCFLMIGICVFNLNSSSCIIKTNAEHCSKRLFSFCYLGVGVIKKPLHLGNRKNMLFLSFLL
  VCLCEEVQMLNLTTTEVSATEFASIASKNMETNVSTSSDYLTGKSETTFSANPETWGKNVTEISIASVAY
  LNQSSMVTSTLAVGTTNRSSGNNVNVTTSSFPTVKGDEAQDIETFFTVILASTLSDVSEKTPQGLPTKST
  PKKTVQALWETDTVQVPELTDTNEGDEEYFKDFLSSLVIWICGISFVGAFIIVIVILYNWYKKDKQRSLL
  WDEENKPDVQIRRDAKTCR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050203
• RefSeq ORF: 897
• Locus ID: 1497100
• MW: 33.2 kDa