U51 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101492

Myc-DDK-tagged ORF clone of viral ORF for G-protein coupled receptor [Human herpesvirus 6], codon optimized for human cell expression, NP_050232

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101492 represents NCBI reference of NP_050232 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGGAAACCAAGTCTCTGGCGTGGCCAGCCACCGCGGAATTTTATGGCTGGGTGTTTATCTTCT
  CCTCTATACAGCTGTGCACTGTGGTGTTCTTGACCGTACGATTCAATGGCTTCAAAGTGGGGCGGGAGTA
  TGCCGTTTTTACATTCGCTGGCATGTCCTTTAATTGCTTTCTGCTCCCGATCAAGATGGGCCTGCTGTCC
  GGCCACTGGACACTTCCAAGGGACTTCTGCGCTATACTCTTGTACATCGATGACTTCAGCGCCTATTTCT
  CAAGCTGGTCATTGGTCTTCATGGCTATTGAGAGAATCAATTATTTCTGCTATAGTACCCCTCTGCTCAA
  TGAAAATTCTAAGGCACTGGCCAAGGTCTGTTTTCCTATAGTGTGGGTAGTGAGCGGTGTCCAGGCCTTG
  CAGATGTTGAATAATTACAAGGCTACAGCCCTTCAGAATGAGACCGGGCAGTGTTTCCTCGCCTTCCTTC
  GGTCTGGACATGATATGTGGCTTATGCTGGTGTATAGCGTGGTGATACCAGTCATGCTGGTCTTTTTTTA
  CCTGTACAGCAAAAACTTCATGCTCCTGAAGGATGAACTTAGTAGTGTGACCACTTACCTTTGTATTTAT
  CTCCTTCTCGGCACCATTGCACACTTGCCGAAGGCAGCTCTGAGCGAGATCGAAAGCGACAAGATCTTTT
  ATGGCCTGCGCGACATATTCATGGCTCTCCCTGTGCTCAAGGTCTACTACATCTCCGCCATGGCATATTG
  CATGGCCTGCGACGACCACACCGTACCCGTGAGGCTTTGCTCCATCTGGCTTGTGAACCTGTGTAAAAAG
  TGTTTCAGCTGTACGCGGAGGGAGAAAGGGAGTGACCTCGAGGTCGGAATAAAGATGCTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101492 representing NP_050232
  Red=Cloning sites Green=Tags
  
  MEKETKSLAWPATAEFYGWVFIFSSIQLCTVVFLTVRFNGFKVGREYAVFTFAGMSFNCFLLPIKMGLLS
  GHWTLPRDFCAILLYIDDFSAYFSSWSLVFMAIERINYFCYSTPLLNENSKALAKVCFPIVWVVSGVQAL
  QMLNNYKATALQNETGQCFLAFLRSGHDMWLMLVYSVVIPVMLVFFYLYSKNFMLLKDELSSVTTYLCIY
  LLLGTIAHLPKAALSEIESDKIFYGLRDIFMALPVLKVYYISAMAYCMACDDHTVPVRLCSIWLVNLCKK
  CFSCTRREKGSDLEVGIKMLK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 903 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050232
• RefSeq ORF: 903
• Locus ID: 1497053
• MW: 34.5 kDa