U38 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101479

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human herpesvirus 6], codon optimized for human cell expression, NP_050219

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101479 represents NCBI reference of NP_050219 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCAGTCTCATTTTTTAACCCGTATCTCGAGGCTAATCGCCTGAAGAAGAAATCCCGGAGTAGTT
  ATATCAGAATCCTGCCCCGAGGGATCATGCACGACGGAGCTGCCGGGCTCATCAAGGACGTGTGTGATAG
  CGAACCAAGGATGTTCTATAGAGACCGACAATATCTGCTTAGCAAGGAAATGACTTGGCCTAGTCTGGAC
  AGAGTGAGAAGTAAGGACTACGACCACACTCGCATGAAGTTCCACATTTATGATGCCGTGGAAACCCTGA
  TGTTCACAGACAGCATTGAAAACCTGCCCTTTCAATACCGCCACTTCGTGATCCCATCTGGCACTGTGAT
  AAGAATGTTCGGACGAAGCGAGGACGGCGAGAAGATTTGTGTGAACGTGTTCGGGCAAGAGCAATATTTC
  TATTGCGAGTGCGTTGACGGCAAATCACTGAAAGCAACCATCAACAACCTGATGCTGACAGGCGAGGTTA
  AAATGTCATGCAGCTTCGTGATTGAACCAGCCGATAAGTTGTCTCTGTATGGATACAACGCCAACACCGT
  GGTTAACCTGTTTAAAGTGAGCTTTGGGAATTTCTACGTTTCCCAGAGAATCGGCAAGATTCTTCAGAAT
  GAGGGATTCGTGGTTTATGAAATTGACGTAGACGTTCTGACCAGATTTTTTGTGGATAATGGGTTTCTGT
  CATTTGGTTGGTATAATGTTAAGAAGTACATTCCCCAGGACATGGGCAAGGGGAGCAATTTGGAGGTGGA
  AATCAATTGTCACGTTTCCGACCTTGTTAGCCTGGAGAACGTGAATTGGCCTCTGTACGGCTGCTGGTCT
  TTTGACATTGAGTGTCTGGGACAGAACGGCAATTTTCCCGACGCCGAGAATCTCGGGGACATTGTGATAC
  AAATTAGTGTGGTGAGTTTCGATACCGAGGGCGACCGGGATGAGCGACATCTGTTTACTTTGGGGACATG
  TGAGCAAATCGACGGCGTGCATATTTATGAGTTCGCCAGTGAGTTTGAGCTCCTTCTGGGGTTTTTTATT
  TTTCTGCGGATCGAGTCACCTGAGTTTATTACAGGCTACAATATCAACAATTTTGACCTTAAATATTTGT
  GCATTCGGATGGACAAGATCTACCACTATGAGATCGGCTGCTTCTCAAAACTCAAAAATGGGAAGATAGG
  CATTAGCGTGCCACACGAACAGTACAAGAAGGGGTTTCTGCAGGCTCAGACAAAAGTCTTCACCTCTGGA
  GTCTTGTATCTCGATATGTACCCAGTCTATAGCTCTAAGATCACCGCCCAGAATTATAAACTCGATACAA
  TCGCAAAAATCTGCCTCCAGCAAGAAAAGGAACAGCTGAGCTATAAGGAAATTCCCAAGAAGTTCATTAG
  CGGCCCTTCTGGGCGGGCCGTAGTTGGCAAGTATTGTCTGCAGGATTCCGTGCTCGTGGTGAGGCTGTTC
  AAGCAGATCAATTATCATTACGAAGTTGCAGAGGTTGCTAGACTGGCACACGTTACAGCACGGTGCGTCG
  TGTTCGAGGGGCAACAGAAGAAAATTTTCCCTTGTATCCTCACCGAAGCGAAAAGACGGAATATGATCCT
  CCCATCCATGGTCTCCTCACACAACAGACAGGGGATCGGGTATAAGGGCGCGACCGTGCTGGAGCCCAAG
  ACCGGTTACTATGCCGTTCCTACCGTTGTGTTTGACTTCCAGAGCCTCTATCCCTCTATAATGATGGCAC
  ACAACCTTTGTTATAGTACTTTGGTGCTGGATGAGAGACAAATCGCTGGACTGAGCGAGAGCGACATCCT
  GACGGTCAAGCTTGGGGATGAAACTCACCGATTTGTGAAGCCATGCGTCAGAGAAAGCGTCCTTGGGTCC
  CTGTTGAAGGATTGGCTGGCCAAGAGAAGAGAAGTGAAAGCAGAAATGCAGAATTGTAGCGATCCGATGA
  TGAAGCTTCTCTTGGACAAAAAACAACTTGCGCTCAAAACTACATGCAACTCCGTGTACGGTGTGACAGG
  AGCTGCACATGGGTTGTTGCCGTGTGTGGCAATTGCCGCCTCAGTGACTTGTCTTGGAAGGGAGATGCTC
  TGTAGTACTGTGGATTACGTGAACAGTAAAATGCAGTCCGAACAGTTCTTCTGCGAGGAACTGGGCCTGA
  CCGCTTCCGATTTCACAGGCGATTTGAAAGTGGAGGTGATATACGGGGATACTGATTCTATCTTTATGTC
  TGTCCGCAATATGGCAAACGAATCACTTCGAAGAATTGCTCCTATGATTGCAAAGCACATAACCGATCGA
  CTCTTCAAAAGCCCAATTAAGCTGGAATTCGAGAAAATCCTGTGTCCGCTGATCCTGATTTGCAAAAAGC
  GGTACATCGGGCGCCAAGATGATAGCCTGCTCATCTTCAAAGGGGTGGACCTCGTGCGCAAGACTAGCTG
  TGACTTTGTCAAAGGAGTTGTCAAAGACATTGTGGACTTGCTGTTCTTTGACGAGGAGGTTCAGACAGCC
  GCTGTCGAATTTTCTCACATGACCCAGACGCAACTCAGGGAGCAGGGTGTTCCTGTTGGGATACACAAAA
  TCCTGCGGCGGCTGTGCAAGGCTAGGGAGGAGCTGTTCCAAAACAGGGCGGACGTGCGCCACCTCATGCT
  GTCCTCTGTCCTGTCCAAGGAAGTAGCCGCGTACAAACAGCCTAACCTCGCTCATCTCTCTGTAATCCGC
  CGCCTGGCTCAGAGGAAGGAGGAGATTCCAAATGTAGGAGATAGAATTATGTATGTGCTGATAGCACCTT
  CTACCGGTAACAAGCAAACCCACAATTACGAGCTGGCAGAGGACCCTAACTACGTCCTGGAACATAAAAT
  CCCTATCCATGCAGAGAAATATTTTGACCAGATCATTAAGGCGGTGACGAATGCGATTTCCCCTATCTTC
  CCTAAGACGGACATAAAGAAGGAAAAGCTGCTGCTGTATCTGTTGCCCATGAAAGTGTACTTGGACGAAA
  CCTTCTCTGCTATAGCCGAAGTCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101479 representing NP_050219
  Red=Cloning sites Green=Tags
  
  MDSVSFFNPYLEANRLKKKSRSSYIRILPRGIMHDGAAGLIKDVCDSEPRMFYRDRQYLLSKEMTWPSLD
  RVRSKDYDHTRMKFHIYDAVETLMFTDSIENLPFQYRHFVIPSGTVIRMFGRSEDGEKICVNVFGQEQYF
  YCECVDGKSLKATINNLMLTGEVKMSCSFVIEPADKLSLYGYNANTVVNLFKVSFGNFYVSQRIGKILQN
  EGFVVYEIDVDVLTRFFVDNGFLSFGWYNVKKYIPQDMGKGSNLEVEINCHVSDLVSLENVNWPLYGCWS
  FDIECLGQNGNFPDAENLGDIVIQISVVSFDTEGDRDERHLFTLGTCEQIDGVHIYEFASEFELLLGFFI
  FLRIESPEFITGYNINNFDLKYLCIRMDKIYHYEIGCFSKLKNGKIGISVPHEQYKKGFLQAQTKVFTSG
  VLYLDMYPVYSSKITAQNYKLDTIAKICLQQEKEQLSYKEIPKKFISGPSGRAVVGKYCLQDSVLVVRLF
  KQINYHYEVAEVARLAHVTARCVVFEGQQKKIFPCILTEAKRRNMILPSMVSSHNRQGIGYKGATVLEPK
  TGYYAVPTVVFDFQSLYPSIMMAHNLCYSTLVLDERQIAGLSESDILTVKLGDETHRFVKPCVRESVLGS
  LLKDWLAKRREVKAEMQNCSDPMMKLLLDKKQLALKTTCNSVYGVTGAAHGLLPCVAIAASVTCLGREML
  CSTVDYVNSKMQSEQFFCEELGLTASDFTGDLKVEVIYGDTDSIFMSVRNMANESLRRIAPMIAKHITDR
  LFKSPIKLEFEKILCPLILICKKRYIGRQDDSLLIFKGVDLVRKTSCDFVKGVVKDIVDLLFFDEEVQTA
  AVEFSHMTQTQLREQGVPVGIHKILRRLCKAREELFQNRADVRHLMLSSVLSKEVAAYKQPNLAHLSVIR
  RLAQRKEEIPNVGDRIMYVLIAPSTGNKQTHNYELAEDPNYVLEHKIPIHAEKYFDQIIKAVTNAISPIF
  PKTDIKKEKLLLYLLPMKVYLDETFSAIAEVM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050219
• RefSeq ORF: 3036
• Locus ID: 1497040
• MW: 115.7 kDa