U53 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101494

Myc-DDK-tagged ORF clone of viral ORF for proteinase [Human herpesvirus 6], codon optimized for human cell expression, NP_050234

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101494 represents NCBI reference of NP_050234 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGGTGTGGGTCGGTGGCTTCCTGTGCGTATACGGGGAGGAGCCTAGCGAGGAGTGTCTGGCCC
  TGCCACGCGACACTGTACAGAAAGAGCTCAGATCCGGAAATATTCCACTGCCCTTGAACATCAACCATAA
  CGAGAAAGCAACAATCGGGATGGTACGGGGCCTTTTCGACCTTGAGCACGGTTTGTTCTGCGTGGCCCAG
  ATCCAGTCCCAGACCTTTATGGATATAATCAGAAACATCGCCTCAAAAAGCAAGCTTATTGCGGCGGGGA
  GTGTAATAGAACCTTTGCCACCCGATCCCGAGATTGAGTGTCTGTCATCATCCTTTCCTGGCCTCTCTCT
  GAGTAGTAAGGTTTTGCAGGACGAGAATCTGGATGGCAAGCCATTCTTCCACCACGTTTCCGTGTGCGGG
  GTCGGCAGGAGGCCCGGTACTATCGCAATTTTTGGGCGCGAGATAAGCTGGATCCTGGACAGATTCTCTT
  GCATTTCCGAGTCCGAGAAAAGACAGGTGCTTGAAGGCGTTAACGTGTACAGTCAGGGGTTCGATGAAAA
  TCTCTTTAGTGCAGATCTTTATGACCTCTTGGCTGACAGCTTGGACACCTCCTACATCCGGAAGAGGTTC
  CCCAAACTGCAGCTTGATAAGCAGCTCTGTGGACTTTCTAAGTGCACGTATATTAAGGCATCCGAACCTC
  CAGTGGAGATAATCGTCGCAACCGGGAAGGTAGCTGGGGATCAGGTCCAGCTGACAACTGAGCCTGGATC
  CGAGCTTGCAGTCGAGACCTGCGATGTGTCTGTTGTGCACGGAAACTATGACGCAGTTGAGTCAGCCACA
  GCGACCACTGCTATGTCCAACCAAAATCTTCCTAATACCACACCACTGCTCTCTTCCCCTCCATTTTCAG
  ATTGTGTCTTCCTGCCAAAAGACGCCTTTTTCTCCCTGCTGAACGTAACAACAGGCCAGCAGCCTAAGGT
  GGTCCCCCCTGTGAGCGTGCACCCACCAGTTACCGAGCAGTACCAGATGCTCCCTTACTCCGAAAGTGCA
  GCAAAAATCGCGGAGCAGGAGTCCAACAGATACCATAGTCCATGTCAGACTATGTATCCCTATTGGCAGT
  ATTCTCCTGTCCCCCAATATCCCGCCGTACTGCATGGTTATAGACAACCGAAAACCTTCAAGAAGAGGCA
  CTTCCAATCCGATAGCGAGGACGAGCTCAGCTTTCCAGGTGATCCTGAGTACACGAAGAAGAGACGGCGG
  CACAAGGTGGACAATGACGATGACAAAGAAATGGCTCGAGAAAAAAATGACCTGAGAGAGCTCGTGGACA
  TGATTGGGATGCTCAGACAAGAGATAAATGCCCTGAAGCACGTGCGAGCACAGTCACCCCAGCGACACGT
  GGTGCCCATGGAGACGCTGCCTACAATAGAGGAGAAAGGTGCGGCAAGCCCAAAGCCATCAATTCTTAAC
  GCCAGTTTGACACCGGAAACCGTGAACAGAAGCCTCGCAGGGCAGAATGAATCCATGGATCTGCTCAAAC
  TGAACAAAAAGCTTTTCGTAGATGCGCTCAATAAAATGGATTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101494 representing NP_050234
  Red=Cloning sites Green=Tags
  
  MSKVWVGGFLCVYGEEPSEECLALPRDTVQKELRSGNIPLPLNINHNEKATIGMVRGLFDLEHGLFCVAQ
  IQSQTFMDIIRNIASKSKLIAAGSVIEPLPPDPEIECLSSSFPGLSLSSKVLQDENLDGKPFFHHVSVCG
  VGRRPGTIAIFGREISWILDRFSCISESEKRQVLEGVNVYSQGFDENLFSADLYDLLADSLDTSYIRKRF
  PKLQLDKQLCGLSKCTYIKASEPPVEIIVATGKVAGDQVQLTTEPGSELAVETCDVSVVHGNYDAVESAT
  ATTAMSNQNLPNTTPLLSSPPFSDCVFLPKDAFFSLLNVTTGQQPKVVPPVSVHPPVTEQYQMLPYSESA
  AKIAEQESNRYHSPCQTMYPYWQYSPVPQYPAVLHGYRQPKTFKKRHFQSDSEDELSFPGDPEYTKKRRR
  HKVDNDDDKEMAREKNDLRELVDMIGMLRQEINALKHVRAQSPQRHVVPMETLPTIEEKGAASPKPSILN
  ASLTPETVNRSLAGQNESMDLLKLNKKLFVDALNKMDS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050234
• RefSeq ORF: 1584
• Locus ID: 1497055
• MW: 58.8 kDa