U64 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101503

Myc-DDK-tagged ORF clone of viral ORF for tegument protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050244

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101503 represents NCBI reference of NP_050244 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACACACTTGTATTATGACACCCTGTACCAGTACCAAGGTGGCGTTTATCCAGCTCACATATGCC
  TTCCAACCGATGCCTACTTGCCTATGCGGGTGGACTGTATAGAATCCTTGTATTTCCGATGTGTCTTTTT
  TAAGAATGGGATGCATTACACAGAATGGTCAAAACTGAAGTTCACCGTGATTTCTAGGGAGATCAAATTT
  AAAGATGTTCTGAAGAACGCAGACTTTGACGAACTCTTCACCGGACTGGTGGTGATGACCATCCCCATAC
  CTATCGTCGATTTCCATTTTGACATCGATAGCGTTATACTCAAGCTCGTATATCCACAGCTGGTCCACCG
  AGAGATCGTCCTGCGGCTGTATGACCTGATCTGTATCAGGCCTTCTTCCGACAGACCAAGCGAGGTCTCC
  GCGAAAAACATCGGAATCGACTTCTATCAGCTTACCAGCCAGGGAAACAAGCAGACCCCCGATGAGGAGA
  AGCGATGTCTGTTTTTCCAACAAGGTCCTTTGGAGCCGCCTAGTACCGTTCGGGGACTGAAGGCAGCCGG
  AAATGCCAAGCCAATGCAGTTCCCCGCTCATGTAAACGAAAAAATGACCGAGAGCTTTCTGTCTGATTCC
  TGGTTTGAACAGAAGGTAAGGTGTAAAAAGATTCTTGATTTCACCCAAACCTATCGCGTGGTGGTGTGCT
  GGTATGAGCTGTCCTTTTCCAGAGAGATGCAAATAGAAAACAATCTGCTGTCAGCCTCTCAGCTGAAAAG
  GGTTAACGCCGCAGACTTTTGGGATAGGACCGACCGGTATCTGAGGGATATCGGATCTCGCGTGCTTACT
  CACATTGTGAAAACACTCCAAATCCATAATAGACAGTTTAAACAGAAGTTCAACTGTAATTTCCCCGTGA
  ACTTCAGTTTCGAGCACCTGCTGAGCTTCATGCAGCTTGGGAAGGACTTTTGGATCCTGAACCTCACTCT
  GGACTCATGCATAATCAAAGCTATTATCTGTTTCCTGGGGTTCCGGAATGGACGGAAGTCCTTCCTGGCC
  CAGGATGAAGTGTGGGGAGACCTTATCGATTGTTCTAAGGGATCAGTTATATATGGCGAGAAAATCCAAT
  GGATCCTCGATAGCACTAACAACCTGTATTCCACCCGGCGCGAAAAACAGAACAAGTCTTGGGAACTGTA
  TGTAGATTGTTGCGCACTGTACGTATCCGAAAAGCTCGAGTTGGATTTTGTCTTGCCAGGCGGCTTCGCA
  ATCACCGGCAAGTTCGCCCTCACAGACGGAGATATCGACTTCTTCAATTGGCGGTTTGGCCTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101503 representing NP_050244
  Red=Cloning sites Green=Tags
  
  METHLYYDTLYQYQGGVYPAHICLPTDAYLPMRVDCIESLYFRCVFFKNGMHYTEWSKLKFTVISREIKF
  KDVLKNADFDELFTGLVVMTIPIPIVDFHFDIDSVILKLVYPQLVHREIVLRLYDLICIRPSSDRPSEVS
  AKNIGIDFYQLTSQGNKQTPDEEKRCLFFQQGPLEPPSTVRGLKAAGNAKPMQFPAHVNEKMTESFLSDS
  WFEQKVRCKKILDFTQTYRVVVCWYELSFSREMQIENNLLSASQLKRVNAADFWDRTDRYLRDIGSRVLT
  HIVKTLQIHNRQFKQKFNCNFPVNFSFEHLLSFMQLGKDFWILNLTLDSCIIKAIICFLGFRNGRKSFLA
  QDEVWGDLIDCSKGSVIYGEKIQWILDSTNNLYSTRREKQNKSWELYVDCCALYVSEKLELDFVLPGGFA
  ITGKFALTDGDIDFFNWRFGLS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050244
• RefSeq ORF: 1326
• Locus ID: 1497064
• MW: 51.6 kDa