U21 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101460

Myc-DDK-tagged ORF clone of viral ORF for putative membrane glycoprotein [Human herpesvirus 6], codon optimized for human cell expression, NP_050201

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101460 represents NCBI reference of NP_050201 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCAGCTTCACAACGGCGTCCAGGTTTACTTTCCTGCTCCTGCCCTTCTTTGTGTTGGCCGGCC
  TTCTGTGGATTCTGTGCCTGATCTGCGGCAGAAACCCATCCACCCTGTGGTACGACGGCGATCTGATTCT
  TAAGATCGCATCTTTGGAACTGGTGGGAGCTTCAGATAGGAAGCGAGAAGGAAAGAGAAGCATGATGATT
  TGTTTCGTGTTCCTGTGCGTCCTCACGTTCGTTCGCGGAGAGATTTATCCTTCCACATGCCCAGCCCTGG
  GAGCCGGCAACGGAGAGGCGGTACGAAGCGGGGAAATGCTCTTGGAAATCTCCGCCTACAGAAACTGGAG
  ATCCGGTAAGATGGAACTCTGGGGCTCTGCAGCCGTAAACAACCAAGTATTCTATGGCGGCATGGAAGAC
  TCTCAGATTGAGTATGACTTCGGAAAATTTCTGGTCTTCCGATGCTTTCAGGTATTTCACAACGTGCACA
  AGCTGCTGTTTAATACAGTGTCCTCTGCTACGATGCATCTGGCAAGGAAGCGGGTGCAGAAATGTGGACA
  TGGCAAAATGACGTTTATCTCCATTCAAGTGCAGTGCAGCGTGAACAAAAAATCCATCCGATTGAGCAGA
  ATGAACGAAACAAACCTTAAGAAGCAAGTCCTTAGAGTGGCCTTTTTTTTGGACGGTTCCAACAACAGCT
  GGATCGCCGACAAGAATTTCCAGGGAGAGGATAGGACGATGCTGCGCCTGTGGACTGAGCTGAGCACGTA
  CAGGCAGTACCTCATTAGCTCTTGTAACAATGACGTGAAGGTTTTGAGCGAGCTGTATGGGGAGTTCCGG
  AGGATGGCCCTGTCATACGACGAGGAACTTAAGCTGAACTTCATGCCAGTTATTCGCAGCTCATCAGAGC
  GCCTCTTCAGGGCTGACGATTTGAAGTGTTCCTTCTCCCGCTGGCTCGGCGCCGAGGGCGAATTCGCCGT
  TTGCGAATACTCCGGTTGGGGTGTGTCCAAGCTCGGCAAGATTGAGATTTTCGCCGAGGAGCCGCTGACA
  TTTGACATGGTCTGGAAGACCGTGAAGATGAGGAGCTCTGGTGCTTACACCAGCCTGTTTCGCGATGACG
  TCACCTGGGGATTGATTAGTTTGGATAAATGGGTTGGCGACAAATACTTCTGCATGTGCACAAACAAAGA
  AAGCGGTGATAACGTGATAGTAACATTGCCCGAGAAAAATGTGGAAAAGTCCATCCAGATTTATAATGAA
  GGATCTACGATGCTGGCCTTTGCTGAAATAACTTCAATTATGGTGAACTTGATGTTTATGGGGGCGGTGG
  CAGTTTGTGTTGGGATCCTGGGAATCAGCTGCTTCGTAGGACTCAAGGAGATAATTTATTTCATCTTCGT
  GTCTGTCAATTCAATGTGGCCCTTCTGTAACAAGCTGCTGACAACAGCCGTTAACTGTTTTTTTAAAGGG
  AGGACGTTCCTCCGGAGGGAGCTTAAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101460 representing NP_050201
  Red=Cloning sites Green=Tags
  
  MSASFTTASRFTFLLLPFFVLAGLLWILCLICGRNPSTLWYDGDLILKIASLELVGASDRKREGKRSMMI
  CFVFLCVLTFVRGEIYPSTCPALGAGNGEAVRSGEMLLEISAYRNWRSGKMELWGSAAVNNQVFYGGMED
  SQIEYDFGKFLVFRCFQVFHNVHKLLFNTVSSATMHLARKRVQKCGHGKMTFISIQVQCSVNKKSIRLSR
  MNETNLKKQVLRVAFFLDGSNNSWIADKNFQGEDRTMLRLWTELSTYRQYLISSCNNDVKVLSELYGEFR
  RMALSYDEELKLNFMPVIRSSSERLFRADDLKCSFSRWLGAEGEFAVCEYSGWGVSKLGKIEIFAEEPLT
  FDMVWKTVKMRSSGAYTSLFRDDVTWGLISLDKWVGDKYFCMCTNKESGDNVIVTLPEKNVEKSIQIYNE
  GSTMLAFAEITSIMVNLMFMGAVAVCVGILGISCFVGLKEIIYFIFVSVNSMWPFCNKLLTTAVNCFFKG
  RTFLRRELKI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050201
• RefSeq ORF: 1500
• Locus ID: 1497017
• MW: 57.0 kDa